用RAPD分析麻核桃起源与分类地位

摘要/Abstract

摘要：

利用随机扩增多态性DNA(RAPD)方法对麻核桃、核桃楸、核桃、奇异核桃和核桃×核桃楸的人工杂种进行了基因组多态性分析。选用28个10 bp随机引物扩增出332个DNA片断,片断大小在259 bp ~ 3054 bp之间,其中243个DNA片断呈现多态性,占总扩增片断的73.2%。依据扩增结果进行相似性系数和遗传距离分析,据此构建聚类树状图。研究结果表明:核桃楸×核桃的天然杂交是麻核桃形成的主要机制;在麻核桃形成过程中,核桃楸的遗传贡献率大于核桃;在胡桃属分类中,麻核桃应归为核桃楸组,这与传统分类学的结果一致。

关键词: 麻核桃, RAPD, 起源, 分类地位

Abstract:

The genomic DNA variations of J. hopeiensis、J. mandshurica、J. regia、J. hidsii ×J. regia and J. regia ×J. mandshurica were analyzed using random amplified polymorphism DNA(RAPD) in the present study. Twenty-eight decanucleotide arbitrary primers generated 332 RAPD bands with the size ranging from 259 bp to 3054 bp, 243 of these bands were polymorphic (73.2 %). The similarities and genetic distances among them were analyzed with Jaccard's and Nei's methods respectively. The dendrogram based on the genetic distances supports the hypothesis that J. hopeiensis originated from the nature hybrid of J. regia and J. mandshurica. In addition, the J. mandshurica gave more genetic contribution than J. regia to the hybrid. Therefore the J. mandshurica is suggested in the Sect. Cardiocaryon, which agrees with the conventional taxonomic position.

Key words: J. hopeiensis, RAPD, Origin, Taxonomic position

吴燕民,裴东,奚声珂,李嘉瑞. 用RAPD分析麻核桃起源与分类地位[J]. 林业科学, 1999, 35(4): 25-30.

Yanmin Wu,Dong Pei,Shengke Xi,Jiarui Li. ANALYSIS OF THE ORIGIN AND THE TAXONOMIC POSITION OF JUGLANS HOPEIENSIS USING RAPD MARKERS[J]. Scientia Silvae Sinicae, 1999, 35(4): 25-30.

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