日本松干蚧3个地理种群的遗传分化

doi:10.11707/j.1001-7488.20131213

林业科学 ›› 2013, Vol. 49 ›› Issue (12): 88-96.doi: 10.11707/j.1001-7488.20131213

日本松干蚧3个地理种群的遗传分化

杨钤¹, 谢映平¹, 樊金华¹, 邵生富², 吴俊², 王彦士³, 赵常胜⁴, 张英伟⁵

1. 山西大学生命科学学院太原 030006;
2. 浙江省金华市林业局金华 321000;
3. 山东省青岛市林业局青岛 266061;
4. 吉林省伊通满族自治县林业局伊通 130700;
5. 辽宁省抚顺市林业局抚顺 113006

收稿日期:2013-01-03 修回日期:2013-03-22 出版日期:2013-12-25 发布日期:2014-01-01
通讯作者: 谢映平
基金资助:
国家自然科学基金项目（31070584）；高等学校博士学科点专项科研基金项目（20101401110008）。

Genetic Differentiation of Matsucoccus matsumurae from Three Geographic Populations in China

Yang Qian¹, Xie Yingping¹, Fan Jinhua¹, Shao Shengfu², Wu Jun², Wang Yanshi³, Zhao Changsheng⁴, Zhang Yingwei⁵

1. School of Life Science, Shanxi University Taiyuan 030006;
2. Jinhua Forestry Bureau, Zhejiang Province Jinhua 321000;
3. Qingdao Forestry Bureau, Shandong Province Qingdao 266061;
4. Yitong Forestry Bureau, Jilin Province Yitong 130700;
5. Fushun Forestry Bureau, Liaoning Province Fushun 113006

Received:2013-01-03 Revised:2013-03-22 Online:2013-12-25 Published:2014-01-01

摘要/Abstract

摘要：

选择我国松干蚧分布的代表性地区——浙江金华（ZJJH）、山东青岛（SDQD）、辽宁抚顺（LNFS）采集松干蚧雌成虫，采用优化的RAPD技术和筛选的4条长度为10 bp的随机引物，测定分析3个地理种群松干蚧的遗传多样性及遗传分化。结果表明：从松干蚧单头雌成虫抽提总DNA可以获得足量用于RAPD-PCR反应的模板DNA，雌成虫总DNA分子质量为9 416 bp；在物种水平上，3次重复的种群间分化系数（G_st）分别为0.207 8，0.191 9和0.207 5，表明总的遗传变异中分别有20.78%，19.19%和20.75%的变异存在于种群之间，79.22%，80.81%和79.25%的变异存在于种群内；3次重复中ZJJH与SDQD的Nei's遗传距离均为最小，分别是0.040 7，0.035 5和0.044 9，SDQD与LNFS的遗传距离均为最大，分别是0.065 0，0.056 2和0.067 3，表明3个地理种群有着很近的亲缘关系，属于同一虫种；基于Nei’s遗传距离，利用UPGMA法进行聚类分析的结果表明3个地理种群都属于同一物种，并且在3次重复中ZJJH种群均与SDQD种群先聚为一簇，说明这2个种群间关系较近。

关键词: 松干蚧, 日本松干蚧, 地理种群, 遗传分化, RAPD分析

Abstract:

The Japanese pine bast scale, Matsucoccus matsumurae(Hemiptera: Coccoidea: Matsucoccidae)is an important invasive pest in pine forests of China. However, the classification and the population genetic differentiation of this species over various distribution regions have been a problem in the pest management decision-making. In order to provide molecular evidence for the species identification and scientific management of the pest, we collected samples from Fushun in Liaoning Province(LNFS), Qingdao in Shandong Province(SDQD) and Jinhua in Zhejiang Province(ZJJH), respectively, and applied RAPD-PCR technigue with 4 random primers to investigate the genetic variation of the three geographic populations. The results showed that the DNA extracted from individual female adult was enough for RAPD-PCR. The genome size of the species was 9 416 bp. At species level, coefficient of genetic differentiation(G_st)of the population with three replicates was 0.207 8, 0.191 9 and 0.207 5, respectively, indicating that there were 20.78%, 19.19% and 20.75% of genetic diversity resided among populations, and 79.22%, 80.81% and 79.25% of genetic diversity within populations. The minimum genetic distance derived from the three replicates was 0.040 7, 0.035 5 and 0.044 9 between ZJJH and SDQD, respectively, and the maximum ones was 0.065 0, 0.056 2 and 0.067 3 between SDQD and LNFS, which suggested that the three geographic populations would be very close in genetic relationship and that belong to the same species. According to the UPGMA dendrograms, the 3 populations could be grouped into two clades and one contained ZJJH and SDQD and the other contained LNFS only, which indicated a closer genetic relationship between the ZJJH and SDQD populations. This research provides a scientific basis for the pest management and has an important significance in using sex pheromones and the natural enemies of the pine bast scale for biological control in the future.

Key words: pine bast scale, Matsucoccus matsumurae, geographic populations, genetic diversity, RAPD

中图分类号:

S718.7

杨钤, 谢映平, 樊金华, 邵生富, 吴俊, 王彦士, 赵常胜, 张英伟. 日本松干蚧3个地理种群的遗传分化[J]. 林业科学, 2013, 49(12): 88-96.

Yang Qian, Xie Yingping, Fan Jinhua, Shao Shengfu, Wu Jun, Wang Yanshi, Zhao Changsheng, Zhang Yingwei. Genetic Differentiation of Matsucoccus matsumurae from Three Geographic Populations in China[J]. Scientia Silvae Sinicae, 2013, 49(12): 88-96.

参考文献

高宝嘉, 李梦, 王春和, 等. 2007. 皱大球蚧(Eulecanium kuwana)和瘤坚大球蚧(Eulecanium gigantean)地理种群的RAPD遗传分化. 生态学报, 27(3): 918-923.
林业部野生动物和森林植物保护司. 1990. 森林植物检疫对象和检疫技术. 长春:吉林科学技术出版社, 14-18.
刘全超. 2012. 草履蚧不同寄主和地理种群分化及遗传多样性研究. 保定:河北农业大学硕士学位论文.
鲁亮, 归鸿. 1995. RAPD技术的特点及其在昆虫分类中的应用. 昆虫学报, 38(1): 117-122.
汤祊德. 1978. 关于松干蚧的讨论及一新种描记. 昆虫学报, 21(2): 164-169.
杨平澜. 1979. 关于我国的日本松干蚧鉴定问题. 昆虫学报, 22(1): 108-114.
杨平澜, 胡金林, 仁遵义. 1976. 中国的松干蚧. 昆虫学报, 19(2): 199-204.
张迎春, 刘波, 郑哲民, 等. 2002. 不同保藏处理的昆虫标本 DNA 提取及其随机扩增多态 DNA 反应. 昆虫学报, 45(5): 693-695.
Booth J M, Gullan P J. 2006. Synonymy of three pestiferous Matsucoccus scale insects(Hemiptera: Coccoidea: Matsucoccidae)based on morphological and molecular evidence. Proceedings of the Entomological Society of Washington, 108(4): 749-760.
Branco M, Jactel H, Silva E B, et al. 2004. Effect of trap design, trap size and pheromone dose on male capture of two pine bast scales species(Hemiptera: Matsucoccidae): implications for monitoring and mass-trapping. Agricultural and Forest Entomology, 6(3): 233-239.
Branco M, Franco J C, Dunkelblum E, et al. 2006. A common mode of attraction of larvae and adults of insect predators to the sex pheromone of their prey(Hemiptera: Matsucoccidae). Bulletin of Entomological Research, 96(2): 179-185.
De Leon J H, Setamou M. 2010. Molecular evidence suggests that populations of the Asian citrus psyllid parasitoid Tamarixia radiata(Hymenoptera: Eulophidae)from Texas, Florida, and Mexico represent a single species. Annals of the Entomological Society of America, 103(1): 100-110.
Foldi I. 2004. The Matsucoccidae in the Mediterranean basin, with a world list of species(Hemiptera: Sternorrhyncha: Coccoidea). Annales de la Société Entomologique de France(N.S.), 40: 145-168.
Frey J E, Frey B. 2008. Molecular identification of six species of scale insects(Quadraspidiotus sp.)by RAPD-PCR: assessing the field-specificity of pheromone traps. Molecular Ecology, 4(6): 777-780.
Hille A, Miller M A, Erlacher S. 2005. DNA sequence variation at the mitochondrial cytochrome oxidase I subunit among pheromotypes of the sibling taxa Diachrysia chrysitis and D. tutti(Lepidoptera: Noctuidae). Zoologica Scripta, 34(1): 49-56.
Hodgson C J, Foldi I. 2006. A review of the Margarodidae sensu Morrison(Hemiptera: Coccoidea)and some related taxa based on the morphology of adult males. Zootaxa, 1263: 1-250.
Hoy M A, Jeyaprakash A, Morakote R, et al. 2000. Genomic analyses of two populations of Ageniaspis citricola(Hymenoptera: Encyrtidae)suggest that a cryptic species may exist. Biological Control, 17(1): 1-10.
Koteja J. 1987. Matsucoccidae(Homoptera, Coccinea), living fossils. Bollettino del Laboratorio di Entomologia Agraria, 43: 41-44.
Koteja J. 1998. Fossil coccids do exist. Inclusion Wrostek, 28: 21.
Koteja J, Azar D. 2008. Scale insects from lower cretaceous amber of Lebanon (Hemiptera: Sternorrhyncha: Coccinea). Alavesia, 2: 133-167.
Kuwana S I. 1907. A new Xylococcus in Japan. Bull Imp Agric Exp Sta, 1(2): 209-212.
Welsh J, Mcclelland M. 1990. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Research, 18(24): 7213-7218.
Williams J G K, Kubelik A R, Livak K J, et al. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research, 18(22): 6531-6535.

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日本松干蚧3个地理种群的遗传分化

Genetic Differentiation of Matsucoccus matsumurae from Three Geographic Populations in China

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