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林业科学 ›› 2007, Vol. 43 ›› Issue (02): 48-51.doi: 10.11707/j.1001-7488.20070209

• 论文及研究报告 • 上一篇    下一篇

落羽杉属树种及其杂交后代亲缘关系的RAPD分析

李涵1,2 殷云龙1 徐朗莱2 於朝广1 陈永辉1   

  1. 1.江苏省·中国科学院植物研究所(南京中山植物园),南京210014;2.南京农业大学生命科学学院南京210095
  • 收稿日期:2005-12-07 修回日期:1900-01-01 出版日期:2007-02-25 发布日期:2007-02-25

Genetic Relationships of Taxodium Species and Their Hybrids Based on RAPD Analysis

Li Han1,2 Yin Yunlong1 Xu Langlai2 Yu Chaoguang1 Chen Yonghui1   

  1. 1.Institute of Botany,Jiangsu Province and Chinese Academy of Sciences Nanjing 210014;2.College of Life Sciences,Nanjing Agriculture University Nanjing 210095
  • Received:2005-12-07 Revised:1900-01-01 Online:2007-02-25 Published:2007-02-25

摘要:

利用随机引物扩增多态性DNA(RAPD)技术,对落羽杉属原种及其杂交后代共13个样本进行亲缘关系鉴定。从100个10bp的随机引物中筛选出13个扩增效果较好的引物,共扩增出92条带,其中72条为多态性带。根据扩增结果进行聚类分析,结果表明杂交F1代中山杉302(落羽杉×墨西哥落羽杉)与母本落羽杉更为接近。在回交后代(中山杉302×墨西哥落羽杉)中,BCF1118与BCF1140相似系数最大;BCF11与母本F1302亲缘关系最近;BCF1118与父本墨西哥落羽杉亲缘关系最近。

关键词: 落羽杉属, 杂交后代, RAPD, 亲缘关系

Abstract:

The genetic relationship of thirteen species and hybrids of Taxodium was analyzed by random amplified polymorphic DNA(RAPD). Thirteen better primers were selected from one hundred arbitrary 10 bp primers. Total 92 bands were produced, with which 72 were polymorphic. According to the cluster analysis, the results showed that the genetic relationship of first crossed generation (F1) Taxodium ‘Zhongshansha 302'(T.distichum×T.mucronatum)was closer to the female parent T. distichum. Among the hybrids of first backcrossed generations(BCF1), the genetic similarity coefficients of RAPD between BCF1118 and BCF1140 was the highest, and BCF11 was closer to the female parent Taxodium ‘Zhongshansha 302’, while BCF1118 was closer to the male parent T. mucronatum.

Key words: Taxodium, hybrids, RAPD, genetic relationship