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林业科学 ›› 2008, Vol. 44 ›› Issue (1): 85-89.doi: 10.11707/j.1001-7488.20080115

• 论文 • 上一篇    下一篇

23个油橄榄品种的RAPD分析*

邱源1,2 韩华柏3 李俊强4 侯春霞1 王永清1   

  1. (1.四川农业大学林学园艺学院 雅安625014; 2.四川省自贡市农业局 自贡643000;3.四川省林业科学研究院 成都610081; 4四川省宜宾职业技术学院 宜宾644003)
  • 收稿日期:2006-10-16 修回日期:1900-01-01 出版日期:2008-01-25 发布日期:2008-01-25
  • 通讯作者: 王永清

RAPD Analysis of 23 Olive (Olea europaea) Cultivars

Qiu Yuan1.2,Han Huabo3,Li Junqiang4,Hou Chunxia1,Wang Yongqing1

  

  1. (1.College of Forestry and Horticulture, Sichuan Agricultural University Ya'an 625014; 2.Zigong Agricultural Bureau, Sichuan Province Zigong 643000; 3.Sichuan Academy of Forestry Chengdu 610081; 4Yibin Vocational and Technical College, Sichuan Province Yibin 644003)
  • Received:2006-10-16 Revised:1900-01-01 Online:2008-01-25 Published:2008-01-25

摘要:

采用RAPD技术对23个引种的油橄榄品种进行分类和鉴定研究。从80个10 bp的随机引物中筛选出11个扩增效果较好的引物进行扩增,共产生127条带,其中78条为多态性带,占61.4%,平均每个引物扩增的DNA多态性带数为7.09条。4个品种具有特异的位点,可作种质鉴定的依据。根据扩增结果构建反映品种间亲缘关系的UPGMA聚类图,23个品种可划分为2大类。

关键词: 油橄榄, RAPD分析, 品种分类, 遗传多样性

Abstract:

Random amplified polymorphic DNA (RAPD) was used to study the classification and identification of 23 olive (Olea europaea) cultivars. Eleven 10 bp primers selected from eighty arbitrary primers were applied for amplifying the olive DNA. Total 127 bands were produced, among which 78 bands (61.4%) were polymorphic. The average numbers of polymorphic DNA bands amplified by each primer were 7.09 There were specific bands in four cultivars which can be used in olive germplasm identification. A dendrogram showing genetic relationships was constructed through an unweighted pairgroup method (UPGMA) based on the DNA polymorphism and the 23 cultivars were clustered into 2 main groups.

Key words: olive (Olea europaea), RAPD analysis, cultivar classification, genetic diversity