林业科学 ›› 2009, Vol. 12 ›› Issue (6): 158-161.doi: 10.11707/j.1001-7488.20090628
• 论文 • 上一篇 下一篇
王亮 王彩虹 田义轲 何晓薇 王辉
收稿日期:
修回日期:
出版日期:
发布日期:
通讯作者:
Wang Liang,Wang Caihong,Tian Yike,He Xiaowei,Wang Hui
Received:
Revised:
Online:
Published:
关键词: 无花果, 叶形性状, RAPD, SCAR
Abstract:
A RAPD marker specific to leaf shape of fig (Ficus carica) was developed and converted into SCAR marker for germplasm identification. RAPD amplifications with random primers S-2082 produced a unique fragment linking to the noncordate base leaf shape. The unique fragment was extracted, cloned and sequenced. Sequencing results indicated that the size of the fragment was 1 640 bp. Specific primers were designed based on its sequence and used in SCAR-PCR, and it was showed that the RAPD marker from S2082-1640 was successfully converted into SCAR marker.
Key words: Fig (Ficus carica), leaf shape, RAPD, SCAR
王亮 王彩虹 田义轲 何晓薇 王辉. 无花果叶形性状的SCAR分子标记*[J]. 林业科学, 2009, 12(6): 158-161.
Wang Liang;Wang Caihong;Tian Yike;He Xiaowei;Wang Hui. A SCAR Molecular Marker Related to Leaf Shape of Fig (Ficus carica)[J]. Scientia Silvae Sinicae, 2009, 12(6): 158-161.
0 / / 推荐
导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks
链接本文: http://www.linyekexue.net/CN/10.11707/j.1001-7488.20090628
http://www.linyekexue.net/CN/Y2009/V12/I6/158