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林业科学 ›› 2009, Vol. 12 ›› Issue (6): 158-161.doi: 10.11707/j.1001-7488.20090628

• 论文 • 上一篇    下一篇

无花果叶形性状的SCAR分子标记*

王亮 王彩虹 田义轲 何晓薇 王辉   

  1. (青岛农业大学园艺学院 青岛266109)
  • 收稿日期:2008-05-14 修回日期:1900-01-01 出版日期:2009-06-25 发布日期:2009-06-25
  • 通讯作者: 王彩虹

A SCAR Molecular Marker Related to Leaf Shape of Fig (Ficus carica)

Wang Liang,Wang Caihong,Tian Yike,He Xiaowei,Wang Hui   

  1. (College of Horticulture, Qingdao Agricultural University Qingdao 266109)
  • Received:2008-05-14 Revised:1900-01-01 Online:2009-06-25 Published:2009-06-25

关键词: 无花果, 叶形性状, RAPD, SCAR

Abstract:

A RAPD marker specific to leaf shape of fig (Ficus carica) was developed and converted into SCAR marker for germplasm identification. RAPD amplifications with random primers S-2082 produced a unique fragment linking to the noncordate base leaf shape. The unique fragment was extracted, cloned and sequenced. Sequencing results indicated that the size of the fragment was 1 640 bp. Specific primers were designed based on its sequence and used in SCAR-PCR, and it was showed that the RAPD marker from S2082-1640 was successfully converted into SCAR marker.

Key words: Fig (Ficus carica), leaf shape, RAPD, SCAR