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林业科学 ›› 2009, Vol. 12 ›› Issue (8): 44-49.doi: 10.11707/j.1001-7488.20090808

• 论文 • 上一篇    下一篇

枣高密度遗传图谱的构建与树干直径的QTL分析

齐靖1 董祯2 毛永民1 申连英1 张玉星1 刘杰1 王晓玲1   

  1. 1.河北农业大学园艺学院保定 071001; 2.河北女子职业技术学院石家庄 050091
  • 收稿日期:2008-09-09 修回日期:1900-01-01 出版日期:2009-08-25 发布日期:2009-08-25
  • 通讯作者: 申连英
  • 基金资助:
     

Construction of a Dense Genetic Linkage Map and QTL Analysis of Trunk Diameter in Chinese Jujube

Qi Jing1,Dong Zhen2,Mao Yongmin1,Shen Lianying1,Zhang Yuxing1,Liu Jie1,Wang Xiaoling1   

  1. 1.College of Horticulture,Agricultural University of HebeiBaoding 071001; 2.Hebei Womens Vocational CollegeShijiazhuang 050091
  • Received:2008-09-09 Revised:1900-01-01 Online:2009-08-25 Published:2009-08-25
  • Supported by:
     

摘要:

以冬枣×临猗梨枣杂交F1代的150株个体为作图群体,利用AFLP标记和RAPD标记,对已有的枣遗传连锁图谱进行加密,构建1张包含388个AFLP标记和35个RAPD标记、由15个连锁群组成的高密度枣遗传连锁图谱,覆盖基因组总长度1 309.4 Cm,标记间平均距离3.1 Cm。与原图谱相比,总图距增加72 Cm,标记间平均距离缩短0.8 Cm,大于10 Cm的标记间隔减少7个,图谱饱和度显著提高。在新构建图谱的基础上,利用JionmapQTL4.0软件,首次对控制枣树干直径性状的QTL进行分析。共检测到控制枣树干直径的QTL 6个,分布在5个连锁群上,分别可解释38.1%,10.0%,14.4%,10.1%,13.4%和31.0%的表型变异值。

关键词: 枣, AFLP标记, RAPD标记, 遗传图谱, 树干直径, QTL分析

Abstract:

In this study,AFLP and RAPD markers were used to increase density of the genetic linkage map of Chinese jujube based on a F1 population of 150 individuals derived from the cross between Dongzao (Zizyphus jujuba ‘Dongzao’) and Linyilizao (Zizyphus jujuba ‘Linyilizao’). As a result,a dense genetic linkage map of Chinese jujube was constructed,and it consisted of 15 linkage groups and covered 1 309.4 Cm,including 388 AFLP markers and 35 RAPD markers. The average interval distance was 3.1 Cm. Compared with the previous linkage map,the total length of map increased by 72 Cm,the average interval distance reduced by 0.8 Cm,and the number of intervals with the distance >10 Cm between adjacent markers declined 33%. Based on the newly constructed genetic linkage map,the QTLs for trunk diameter in jujube was also analyzed in this study,and a total of 6 QTLs were identified,and they could explain 38.1%,10.0%,14.4%,10.1%,13.4% and 31.0% phenotypic variation respectively.

Key words: Chinese jujube (Zizyphus jujuba), AFLP marker, RAPD marker, genetic linkage map, trunk diameter, QTL analysis

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