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林业科学 ›› 2010, Vol. 46 ›› Issue (3): 56-61.doi: 10.11707/j.1001-7488.20100309

• 论文 • 上一篇    下一篇

核桃早实基因的SCAR标记

李伟波1,2 马明3 孙翠1,2 侯立群4 杨克强1,2   

  1. 1.作物生物学国家重点实验室泰安 271018; 2.山东农业大学农业生态与环境重点实验室泰安 271018; 3.山东省济宁技术学院济宁 272013; 4.山东省林业科学研究院济南 250014
  • 收稿日期:2008-12-18 修回日期:1900-01-01 出版日期:2010-03-25 发布日期:2010-03-25
  • 通讯作者: 杨克强

Development of a SCAR Marker Linked to Precocious Trait in Walnut (Juglans regia)

Li Weibo1,2,Ma Ming3,Sun Cui1,2,Hou Liqun4,Yang Keqiang1,2   

  1. 1.State Key Laboratory of Crop BiologyTai’an 271018; 2.Key Laboratory of Agricultural Ecology and Environment, Shandong Agricultural University Tai’an 271018; 3.Jining Technology College, Shandong ProvinceJining 272013;4.Shandong Academy of ForestryJinan 250014
  • Received:2008-12-18 Revised:1900-01-01 Online:2010-03-25 Published:2010-03-25

摘要:

根据与核桃早实性状连锁的RAPD标记OPB-08958序列,设计SEA-F/SEA-R和SEB-F/SEB-R 2对SCAR引物。在亲本、杂交后代和栽培品种(系)中的检测结果表明:SEA-F/SEA-R引物对在早实亲本‘绿园’中能稳定扩增出SCAR-SEA958特异标记; 在‘绿园’ב绿丰’杂交F1后代群体中,SCAR-SEA958标记与核桃早实性状共分离,与核桃早实性状间的遗传距离为1.99 cM; 用晚实优系‘T-12’和栽培品种‘元丰’及‘T-12’ב元丰’杂交F1后代,检测SCAR-SEA958标记的分离情况,SCAR-SEA958与核桃早实性状共分离; 在9个核桃栽培品种(系)中,SCAR-SEA958在8个供试品种的7个早实品种上出现,在1个早实品种‘鲁光’和1个晚实优系‘T-12’上没有扩增出条带。SCAR-SEA958标记在不同遗传背景下有较高的稳定性。

关键词: 核桃, 早实性状, RAPD, SCAR 柳杉, 细根衰老, 养分内循环, 养分迁移

Abstract:

SCAR(sequencecharacterized amplified region)marker primers SEA-F/SEA-R and SEB-F/SEB-R were designed on the basis of the DNA sequence of RAPD marker OPB-08958 to obtain the SCAR marker linked to the precocious gene in walnut(Juglans regia). The reliability of SCAR-SEA958 marker was detected by screening DNA from parents,hybrids and varieties(clone) with SEA-F/SEA-R primers. The results showed that the specific marker SCAR-SEA958 was amplified stably using DNA extracted from precocious parent ‘Lüyuan’ as the template with SEA-F/SEA-R primers. In the F1 hybrid population from ‘Lüyuan’בLüfeng’,the SCAR-SEA958 marker was co-segregating with precocious gene of walnut and the genetic distance between SCAR-SEA958 and precocious gene was 1.99 cM. To detect the reliability of SCAR-SEA958 marker further, the template DNA from later mature maternal parent ‘T-12’, precocious paternal parent ‘Yanfeng’ and their 123 F1 hybrids plants was screened with SEA-F/SEA-R primers. The SCAR-SEA958 marker was co-segregating with precocious gene in the F1 hybrid population from ‘T-12’בYanfeng’ either. In 9 walnut varieties(clone),the SCAR-SEA958 marker was presented in 7 out of 8 precocious varieties, but absent in precocious variety ‘Luguang’ and later mature clone ‘T-12’.It was indicated that SCAR-SEA958 would be an identifier of precocious walnut varieties(clone). Stability of the effect of SCAR-SEA958 was high in different genetic backgrounds.

Key words: walnut (Juglans regia), precocious trait, RAPD, SCAR