关键词: 关键词：RAPD, SSR, 濒危灌木, 长叶红砂, 遗传多样性

Abstract: Abstract: RAPD and ISSR markers were applied to detect the genetic diversity of 5 endangered shrub Reaumuria trigyna populations. Eighteen RAPD and fourteen ISSR primers generated 118 and 114 loci, of which 88.98% and 89.47% were polymorphic respectively. At species level, the results of RAPD indicated that Shannon's index diversity(I) was 0.465 6, and Nei's index (H) was 0.330 3. The results of ISSR markers showed that I and H was 0.468 8 and 0.308 4 respectively. High level genetic diversity of the endangered shrub was detected by the two markers. Nei’s gene diversity showed that there were major genetic variations within populations. Genetic variations within populations were 86.22% by RAPD and 89.29% by ISSR. There was strong gene flow among populations (Nm was 3.009 7 by RAPD and 4.178 7 by ISSR), therefore low genetic variations were found among populations. Comparison between the two markers showed that ISSR was better than RAPD in terms of reproducibility and ability of detecting genetic polymorphism. The level of higher genetic diversity was related to species traits and adaptation to stress habitat. The results showed endangered plants was not necessary to display lower level of genetic variation.

Key words: Key words: RAPD, ISSR, endangered shrub, Reaumuria trigyna, genetic diversity