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林业科学 ›› 2008, Vol. 44 ›› Issue (5): 155-159.doi: 10.11707/j.1001-7488.20080528

• 论文 • 上一篇    下一篇

基于RAPD标记的白桦遗传连锁群分析*

李绍臣 高福玲 姜廷波   

  1. (东北林业大学 林木遗传育种与生物技术教育部重点实验室 哈尔滨150040)
  • 收稿日期:2007-04-25 修回日期:1900-01-01 出版日期:2008-05-25 发布日期:2015-04-22
  • 通讯作者: 姜廷波

Analysis of Genetic Linkage Groups on Birch Using RAPD Markers

Li Shaochen,Gao Fuling,Jiang Tingbo   

  1. (Key Laboratory of Forest Tree Improvement and Biotechnology of Ministry of Education Northeast Forestry University Harbin 150040)
  • Received:2007-04-25 Revised:1900-01-01 Online:2008-05-25 Published:2015-04-22

关键词: 白桦, 欧洲白桦, RAPD标记, 分子遗传图谱, 拟测交

Abstract:

Based on the genetic inheritance and segregation of random amplified polymorphism DNA (RAPDs) markers,the analysis of linkage groups for birch was performed by using a pseudo testcross strategy. A segregating population comprising of 79 progenies from the cross between Betula pendula and B. platyphyllawas obtained. A set of 1 000 random oligonucleotide primers were screened,and 178 primers were selected to generate RAPD markers within a sample of 79 F\-1 progenies. A total of 296 segregating sites was identified. Among them,273 sites belonged to 1∶1 segregating site,and 36 sites were found distorted from the 1∶1 ratio. The 273 sites segregating 1∶1 (testcross configuration) were used to construct parentspecific linkage group,among which 137 sites were found for B. pendula and 136 sites for B. platyphylla. The resulting linkage maps consisted of 117 marker sites in 12 groups (four or more sites per group),2 triples and 3 pairs for B. pendula,which covered the map distance about 1 533.3 cM (Kosambi units),and the average map distance between adjacent markers was 16.4 cM. In the B. platyphylla,162 linked marker sites were integrated into 12 groups (four or more sites per group),2 triples and 4 pairs,which covered the map distance about 1 847.8 cM,and the average map distance between adjacent markers was 19.2 cM. The calculated genome coverage rate of B. pendula and B. platyphylla were respectively 68.5% and 68.9%. Further study is required to integrate the two maps to one density map and to locate important genes on the maps.

Key words: Betula platyphylla, Betula pendula, RAPD markers, genetic linkage group, pseudo testcross