关键词: 外生菌根蘑菇, 分离菌株, DNA鉴定, RAPD

Abstract:

Tissue isolation for mycelia was conducted from ectomycorrhizal mushrooms Suillus granulatus (Fr.) Kuntze and Scleroderma areolatum Ehrenb. collected in Jilin province and Russula rubra (Krombh) Bres. from Fujian province. The results showed that position of basidiocarps and kind of media were two of the factors determining the success of isolation. The DNA fingerprinting comparison was made to identify the isolates in this study. The results indicated that basidiocarps of Suillus granulatus from different sources were above the level of 0 939 DNA similarity coefficient, however, their mycelia isolates all had the same DNA fingerprinting patterns of RAPD (Random Amplified polymorphic DNA) using 10 arbitrary decamer nucleotide primers screened as their origin basidiocarps respectively, whose DNA similarity coefficients all were 1 000, and were therefore identified as true culture of the mushroom. In addition, the mycelia isolated from basidiocarps of Sclerodema areolatum were also authenticated as true isolates, but the cultures from a spawn unit of Fujian province had significant DNA heterogeneity with tested basidiocarps of Russula rubra, and were dertermined to be false isolates.

Key words: Ectomycorrhizal mushroom, Isolate, DNA identification, Fingerprinting comparsion