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林业科学 ›› 2007, Vol. 43 ›› Issue (12): 50-55.doi: 10.11707/j.1001-7488.20071209

• 论文及研究报告 • 上一篇    下一篇

杉木第2代育种群体优良基因型的RAPD遗传变异(英文)

李梅1,2 施季森2 李发根1 甘四明1   

  1. 1.中国林业科学研究院热带林业研究所,广州510520;2.南京林业大学国家林业局林木遗传与基因工程重点实验室,南京210037
  • 收稿日期:2007-05-11 修回日期:1900-01-01 出版日期:2007-12-25 发布日期:2007-12-25

Molecular Characterization of Elite Genotypes within a Second-Generation Chinese Fir (Cunninghamia lanceolata) Breeding Population Using RAPD Markers

Li Mei1,2,Shi Jisen2,Li Fagen1,Gan Siming1   

  1. 1.Research Institute of Tropical Forestry,CAF Guangzhou 510520; 2.Key Laboratory of Forest Tree Genetics and Gene Engineering of State Forestry Administration Nanjing Forestry University Nanjing 210037
  • Received:2007-05-11 Revised:1900-01-01 Online:2007-12-25 Published:2007-12-25

摘要:

利用RAPD标记研究选自杉木第2代育种试验林、拟纳入高世代育种的182个优良基因型的遗传变异。29条随机引物扩增出311条谱带,其中多态性谱带245条(78.8%)。聚类分析表明,182个优良基因型可聚为11类。AMOVA分析表明,96.8%的遗传变异存在于第2代育种试验林内,而试验林间的遗传变异虽然显著(Φst=0.032,P<0.001),但比例较低(3.2%)。研究结果表明,杉木第2代育种群体存在高水平的遗传多样性,显示了进一步育种的良好潜力。

关键词: 杉木, 优良基因型, 遗传变异, RAPD

Abstract:

RAPD markers were used to characterize the genetic diversity and relationships among 182 elite genotypes selected from second-generation trials as breeds for advanced generation breeding in Chinese Fir (Cunninghamia lanceolata). Twenty-nine 10-mer primers generated a total of 311 fragments,245 (78.8%) of which were polymorphic. Cluster analyses allowed for all 182 genotypes to be grouped into 11 clusters. AMOVA analyses showed that a high proportion (96.8%) of total genetic diversity resided within the field trials from which the elite genotypes were selected,whereas genetic diversity among field trials was low but significant (Φst =0.032,P<0.001). These results demonstrate that high levels of genetic diversity exist within the second-generation breeding population of Chinese Fir and hence suggest that there is good potential for genetic gains from further breeding.

Key words: Chinese Fir (Cunninghamia lanceolata), elite genotype, genetic diversity, RAPD