欢迎访问林业科学,今天是

林业科学 ›› 2005, Vol. 41 ›› Issue (2): 174-177.doi: 10.11707/j.1001-7488.20050230

• 研究简报 • 上一篇    下一篇

栓皮栎体胚诱导关键影响因素研究

张存旭 姚增玉 赵忠   

  1. 西北农林科技大学,杨凌712100
  • 收稿日期:2004-07-15 修回日期:1900-01-01 出版日期:2005-03-25 发布日期:2005-03-25

Factors Influencing the Induction of Somatic Embryogenesis in Quercus variabilis

Zhang Cunxu,Yao Zengyu,Zhao Zhong   

  1. Northwest Sci-Tech University of Agriculture and Forestry Yangling 712100
  • Received:2004-07-15 Revised:1900-01-01 Online:2005-03-25 Published:2005-03-25

关键词: 栓皮栎, 未成熟合子胚, 体胚发生

Abstract:

Immature zygotic embryos of Quercus variabilis were as explants to induce somatic embryogenesis. Several factors influencing somatic embryogenesis have been assayed. Somatic embryos can be induced in MS and WPM basal medium, but there was more quantity, big size and high induction rate in MS medium. Induction rate was not significant cultured in light and dark condition. Zygotic embryos, collected in middle of July, gave higher rate of somatic embryogenesis than those collected on the earlier or later date. By adding 6-BA in medium individually, somatic embryogenesis appeared directly on the zygotic embryos without detectable callus. Secondary embryogenesis appeared in medium with 2,4-D individual or combined with 6-BA or TDZ. High induction frequency of 90% was achieved in MS medium supplemented with 0.5 mg·L-1 6-BA and 2,4-D, whereas the rate in hormone-free medium was only 16.7%. The genotypes of mother trees had an great impact on the inducing rate. Zygotic embryo surgery treatments were not favorable to embryogenesis. It was best to inoculate with entire zygotic embryos. The hypocotyl was a crucial part on somatic embryogenesis for Q. variabilis.

Key words: Quercus variabilis, immature zygotic embryo, embryogenesis