褐马鸡圈养种群ISSR标记的遗传多样性

doi:10.11707/j.1001-7488.20131114

Abstract

Abstract:

Brown-eared Pheasant (Crossoptilon mantchuricum) is a critically endangered endemic species, which is listed in the national first-class protected birds in China. In this study, ISSR markers were used to investigate the genetic diversity of two populations of Brown-eared Pheasant in the Pangquangou Nature Reserve and Taiyuan Zoo. Ten primers out of 20 ISSR primers were selected for amplifying 35 samples of the two populations, and 65 clear amplification sites (PPB=76.9%) were obtained.The percentage of polymorphic bands of the two populations was around 54.10%.Shannon's Information Index of the two populations was 0.302 5 and 0.205 6, respectively. The generic heterogeneity (G_st), genetic flow (N_m), genetic similarity index indicated that there was no significant genetic differentiation between the two populations. Statistical analysis and cluster tree diagram consistently illustrated that the genetic diversity between the two populations was at a low level. Individuals in each population had close genetic relationship. The result in this study were consistent to another study we did with the mitochondrial genome genetic diversity.

Key words: Brown-eared Pheasant, endangered species, populations, ISSR, genetic diversity

CLC Number:

S718.63

Wu Yuzhen, Feng Ruizhi. ISSR Analysis of Genetic Diversity of Two Brown-Eared Pheasant (Crossoptilon mantchuricum) Populations[J]. Scientia Silvae Sinicae, 2013, 49(11): 103-108.

References

Arcade A, Anselin F, Faivre Rampant P, et al. 2000. Application of AFLP, RAPD and ISSR markers to genetic mapping of European and Japanese larch. Theoretical and Applied Genetics, 100(2): 299-307.
Aytekin I, Ozdil F, Zulkadir U, et al. 2011. Evaluation of ISSR markers for genetic diversity analysis in Anatolian Water Buffaloes. Journal of the Science of Food and Agriculture, 91(11): 1957-1962.
Bai X J. 2004.ISSR fingerprint for Manchurian Tigers in captivity. Acta Theriologica Sinica, 24(1): 90-92(in Chinese).
Bai X J, Li H. 2001.Study on ISSR fingerprinting of broiler chicken divergently selected for very low density lipoprotein (VLDL).Journal of Jinlin Agricultural University, 23(1): 80-87(in Chinese).
Casasoli M,Mattioni C,Cherubini M,et al.2001.A genetic linkage map of European chestnut (Castanea sativa Mill.) based on RAPD,ISSR and isozyme markers.Theoretical and Applied Genetics, 102(8):1190-1199.
Curole J P,Kocher T D.1999.Mitogenomics:digging deeper with complete mitochondrial genomes. Trends in Ecology and Evolution, 14(10):394-398.
Fang D Q, Roose M L.1997.Identification of closely related citrus cultivars with inter-simple sequence repeats markers. Theoretical and Applied Genetics, 95(3): 408-417.
Gilbert J E, Lewis R V,Wilkinson M J, et al. 1999. Developing an appropriate strategy to assess genetic variability in plant germplasm collections.Theoretical and Applied Genetics, 98(6/7): 1125-1131.
Hizer S E,Dhar A K,Klimpel K R,et al.2002.RAPD markers as predictors of infectious hypodermal and hematopoietic necrosis virus (IHHNV) resistance in shrimp (Litopenaeus stylirostris).Genome, 45(1):1-7.
Huang J C, Sun M. 2000. Genetic diversity and relationship of sweetpotato and its wild relatives in Ipomoea series Batatas (Convolvulaceae) as revealed by inter-simple sequence repeat (ISSR) and restriction analysis of chloroplast DNA. Theoretical and Applied Genetics, 100(7):1050-1060.
Jin Z X,Li J M, Li Z H.2007.ISSR analysis of the genetic diversity of Torreya jackii Chun natural populations in Xianju County, Zhejiang Province. Journal of Beijing Forestry University, 29(1):53-58(in Chinese).
Kostia S,Ruohonen-Lehto M,Vainola R.2000.Phylogenetic information in inter-SINE and inter-SSR fingerprints of the Artiodactyla and evolution of the Bov-tA SINE. Heredity, 84(1):37-45.
Li J, Li M, Nie L, et al. 2007. Analysis of parents and their hybrids of Antheraea pernyi with ISSR technique. Science of Sericulture, 33 (1): 113-116(in Chinese).
Moritz C.1991.The origin and evolution of parthenogenesis in Heteronotia binoei (Gekkonidae):evidence for recent and localized origins of widespread clones. Genetics, 129(1):211-219.
Noroozi M,Hishamuddin O,Soon G T,et al.2011.Studies on the genetic variation of the green unicellular Alga Haematococcus pluvialis (Chlorophyceae) obtained from different geographical locations using ISSR and RAPD molecular marker. Molecules, 16(3): 2599-2608.
Ostberg C O,Rodriguez R J.2002.Novel molecular markers differentiate Oncorhynchus mykiss (rainbow trout and steelhead) and the O. clarki (cutthroat trout) subspecies. Molecular Ecology Notes, 2(3):197-202.
Parsaeian M, Mirlohi A, Saeidi G. 2011. Study of genetic variation in sesame (Sesamum indicum L.) using agro-morphological traits and ISSR markers. Russian Journal of Genetics, 47(3):314-321.
Pharmawati M, Yan G, Finnegan P M.2005.Molecular variation and fingerprinting of Leucadendron cultivars (Proteaceae) by ISSR markers. Annals of Botany, 95(7):1163-1170.
Sanker A A, Moore G A.2001.Evalution of inter-simple sequence repeat analysis for mapping in Citrus and extension of the genetic linkage map. Theoretical and Applied Genetics, 102(2):206-214.
Sica M,Gamba G,Montieri S,et al. 2005.ISSR markers show differentiation among Italian populations of Asparagus acutifolius L. BMC Genetics, 6(1):17.
Willis P M, Crespi B J, Dill L M,et al. 2004.Natural hybridization between Dall’s porpoises(Phocoenoides dalli)and harbour porpoise(Phocoena phocoena).Canadian Journal of Zoology, 82(5):828-834.
Wright S.1931. Evolution in Mendelian populations.Genetics, 16(2):97-159.
Wu Y Z, Wang M B, Zang F. 2010. D-loop sequence variation of Mitochondrial DNA in captive Brown-eared Pheasant (Crossoptilon mantchuricum).Acta Ecologica Sinica, 30(11): 2958-2964(in Chinese).
Yeh F C,Yang R C,Boyle T B J,et al. 1997. POPGENE Version 1.31,the user-friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Centre,University of Alberta. Edmonton, Canada.
Zhao Q, Du H, Zhuang D H, et al. 2008. Genetic relationships analysis of 14 cultivars of Phalaenopsis hybrid based on ISSR molecular markers. Bulletin of Botanical Research, 28(2): 227-231 (in Chinese).
Zheng F, Lu X L, Sun H Y, et al. 2007. Application of ISSR markers in germplasm analyzing of mitten crab. Journal of Fishery Sciences of China, 14(1):46-51(in Chinese).
Zietkiwicz E, Rafalski A, Labuda D. 1994. Genome fingerprinting by simple sequence repeat(SSR)-anchored polymerase chain reaction amplification. Genomics, 20(2): 176-183.

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ISSR Analysis of Genetic Diversity of Two Brown-Eared Pheasant (Crossoptilon mantchuricum) Populations

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