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Scientia Silvae Sinicae ›› 2014, Vol. 50 ›› Issue (9): 51-58.doi: 10.11707/j.1001-7488.20140907

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A Preliminary Study on Construction of High-Quality Core Collection of Populus nigra

Zeng Xianjun1, Li Dan1, Hu Yanpeng2, Huang Qinjun1, Su Xiaohua1   

  1. 1. Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration Research Institute of Forestry, CAF Beijing 100091;
    2. Longhua State Forest Farm Administration of Hebei Province Chengde 068150
  • Received:2014-04-14 Revised:2014-07-15 Online:2014-09-25 Published:2014-09-30
  • Contact: 黄秦军

Abstract:

In this study, we analyzed 13 traits of 159 Populus nigra germplasm such as growth, breeding value, material properties, efficiency of water use efficiency and efficiency of nutrient utilization. A high-quality core collection of P. nigra was constructed by utilizing 13 kinds of sampling methods of phenotypic selection. Phenotypic value evaluation showed that the sampling rate reached to more than 21.4% can meet the requirement of building core collection. However, the proportion of the sample population over 30.2% was required for building the core collection in terms of SSR molecular genetic diversity. Ultimately, the determined high-quality core collection of P. nigra contained 48 germplasm resources, accounting for 30.2% of the original germplasm. Compared with the original germplasm, there were no differences in the aspects of the mean and the variance, and the coincidence rate of range and the changeable rate of coefficient of variation were 97.07% and 89.72%. The high-quality core collection was no significantly different from the original group in the aspects of the effective number of alleles, Nei’s genetic diversity coefficient and Shannon's information index, suggesting that the high-quality core collection should maintain the genetic diversity of the original group.

Key words: Populus nigra, high-quality core collection, sampling method, genetic diversity

CLC Number: