枣实蝇特异引物PCR鉴定技术

doi:10.11707/j.1001-7488.20131113

Scientia Silvae Sinicae ›› 2013, Vol. 49 ›› Issue (11): 98-102.doi: 10.11707/j.1001-7488.20131113

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Species-specific PCR Primers for Identification of Carpomyia vesuviana

Cheng Xiaotian¹, Adili Shataer¹, Zhang Wei³, Wen Junbao², Li Xinquan¹, Chen Meng⁴

1. Key Lab.of Forestry Ecology and Industry Technology in Arid Region, Education Department of Xinjiang College of Forestry and Horticulture, Xinjiang Agricultural University Urumqi 830052;
2. Key Laboratory for Silviculture and Forest Conservation of Ministry of Education, Beijing Forestry University Beijing 100083;
3. Xinjiang Entry-Exit Inspection and Quarantine Bureau Urumqi 830083;
4. Master Station of Prevention and Quarantine of Forestry Plant Diseases and Insect Pests in Xinjiang Urumqi 830000

Received:2013-06-01 Revised:2013-09-20 Online:2013-11-25 Published:2013-11-26

Abstract

Abstract:

In this study SS-PCR and agarose gel electrophoresis technique were used to select a pair of specific primers from the 15 pairs of primers of designed for Carpomyia vesuviana. Five fruit fly species, Bactrocera dorsalis, B.cucurbitae, B.tau, B. correcta and B.zonata, were used to determine the primer specificity. A series of genomic DNA dilution of C.vesuviana, 40, 20, 10, 1, 0.1, 0.01, and 0.001 ng·μL^-1, were used to detect the sensitivity of SS-PCR. The results showed that the detection limit of SS-PCR was less than 0.01 ng·μL^-1, and the optimum template DNA concentration was between 1 ng and 20 ng·μL^-1. The designed primers could be used for amplifying the DNA of C.vesuviana at different stages. The developed SS-PCR method can be used for identification, inspection and monitoring of C.vesuviana, which would have an important significance in preventing the insect from spreading any further.

Key words: SS-PCR, Carpomyia vesuviana, agarose gel electrophoresis, identification

CLC Number:

Q965

Cheng Xiaotian, Adili Shataer, Zhang Wei, Wen Junbao, Li Xinquan, Chen Meng. Species-specific PCR Primers for Identification of Carpomyia vesuviana[J]. Scientia Silvae Sinicae, 2013, 49(11): 98-102.

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Species-specific PCR Primers for Identification of Carpomyia vesuviana

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