应用微卫星分子标记估算毛竹的异交率

doi:10.11707/j.1001-7488.20140811

Abstract

Abstract:

Mating system is the basic property of plant populations and biological foundation for germplasm preservation as well as genetic improvement. In this study we applied developed 8 microsatellite loci with high polymorphism to analyze 50 half-sib progenies from each of 3 independent flowering culms of Phyllostachys edulis. The results showed that flowering culms in three different locations were fertilized with foreign pollen, which generated progenies with different microsatellite genotypes and genetic diversity. The overall estimates of outcrossing rate t_m of single-locus and t_s of multi-locus for the three culms were 0.089, indicating that the reproductive system of P. edulis predominantly involved in self-fertilization with a certain proportion of outcrossing to produce genetic diverse progenies. This is the first report so far on estimation of outcrossing rate for monopodial bamboo species such as P. edulis by DNA molecular markers.

Key words: Phyllostachys edulis, microsatellite, genetic diversity, mating system, rate of outcrossing

CLC Number:

S718.46

Liu Zhengjie, Lin Yuan, Zhou Mingbing, Tang Dingqin. Estimation of Outcrossing Rate in Phyllostachys edulis by Microsatellite Polymorphism[J]. Scientia Silvae Sinicae, 2014, 50(8): 76-81.

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Estimation of Outcrossing Rate in Phyllostachys edulis by Microsatellite Polymorphism

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