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林业科学 ›› 2008, Vol. 44 ›› Issue (7): 43-49.doi: 10.11707/j.1001-7488.20080708

• 论文 • 上一篇    下一篇

外生菌根真菌产生植物激素差异及其与NL-895杨生长的关系*

吴小芹 马 磊   

  1. (南京林业大学森林资源与环境学院 南京210037)
  • 收稿日期:2006-10-23 修回日期:1900-01-01 出版日期:2008-07-25 发布日期:2008-07-25

Relationship between Plant Hormone Level Excreted by Ectomycorrhizal Fungi and Growth of NL-895 Poplar

Wu Xiaoqin,Ma Lei   

  1. (College of Forest Resources and Environment,Nanjing Forestry University Nanjing 210037)
  • Received:2006-10-23 Revised:1900-01-01 Online:2008-07-25 Published:2008-07-25

摘要:

选用9种外生菌根菌对NL-895杨进行菌根化试验,并采用HPLC法,分别测定各菌根菌在离体培养条件下培养液和菌丝体中的吲哚乙酸(IAA)、玉米素(Z)、赤霉素(GA)和脱落酸(ABA)含量。结果表明:9种菌根菌对NL-895杨苗木生长的影响存在一定差异,从菌根菌对苗木的促生效果来看,无论是否形成明显的菌根关系,接菌苗的生长势均优于对照苗;9种菌根菌均可不同程度地分泌4种植物激素;其培养液中激素含量明显高于菌丝体中的含量;各激素对苗木生长的影响不尽相同。相关分析表明:菌根菌菌丝体中的玉米素(Z)含量与NL-895杨苗高和茎粗呈显著的正相关关系,而菌丝体中的脱落酸(ABA)和吲哚乙酸(IAA)含量则与NL-895杨苗高和茎粗呈显著的负相关关系。

关键词: 外生菌根真菌, 植物激素, NL-895杨, 高效液相色谱

Abstract:

In the present work,the effects of nine ectomycorrhizal fungi on the growth of Populus×euramericanacv. ‘NL-895' poplar was investigated. Using HPLC method,the contents of auxin (IAA),zeatin(Z),gibberellin (GA) and abscisic acid (ABA) of the filtrate and mycelium of nine ectomycorrhizal fungi cultured in liquid medium were also examined. The results showed that the impacts of nine ectomycorrhizal fungi on the growth of NL-895 poplar were significantly varied. The inoculated seedlings grew better than that without inoculation even if mycorrhiza didn't form. All nine ectomycorrhizal fungi to some extent excreted plant hormones,and the contents in culture filtrate were significantly higher than that of mycelium. There were significant differences among the effects of different hormones on seedling growth. Correlation analysis suggested that the height and stem diameter of seedlings were positively correlated with zeatin contents of mycelium,while the contents of ABA or IAA in the mycelium were negatively correlated with the seedling growth.

Key words: ectomycorrhizal fungi, plant hormone, NL-895 poplar, HPLC