野生沉香的鉴别方法

doi:10.11707/j.1001-7488.20170911

摘要/Abstract

摘要： [目的]建立有效、准确、科学的野生沉香鉴别技术，为沉香的鉴定和品质评价体系建立提供参考依据。[方法]采用醇溶性浸出物含量、显色反应、薄层色谱分析（TLC）和高效液相色谱（HPLC）方法，对10批次野生沉香进行鉴别。[结果]10批次野生沉香的醇溶性浸出物含量整体较高，明显大于《中华人民共和国药典》（2015年版）规定的10.0%；野生沉香的显色反应除呈现人工沉香的樱红色或浅樱红外，还出现紫堇色、浅红色和浅紫色在内的多样性显色，可辅助鉴别野生沉香；薄层色谱操作简单，节省材料，且野生沉香样品在薄层鉴别中呈现清晰的荧光斑点和较好的分离效果，对于大部分野生沉香样品可通过薄层色谱进行基础鉴别；通过相似度评价、峰面积比较，建立10批次野生沉香的HPLC指纹图谱，并确定9个野生沉香共有特征峰，确定野生和人工沉香HPLC特征指纹图谱的差异性。[结论]基于各方法的优势及精准程度，本文提出在显微鉴别的基础上，通过醇溶性浸出物含量、显色反应、薄层色谱分析和HPLC的指纹图谱及特征峰鉴别野生沉香，可为沉香品质评价体系建立提供参考。

关键词: 野生沉香, 鉴别方法, 高效液相色谱, 指纹图谱, 共有特征峰

Abstract: [Objective] This paper focused on the establishment of effective, accurate and scientific wild agarwood identification technology, and will help to offer a reference basis for the quality evaluation of agarwood standard.[Method] Using alcohol soluble extract content, color reaction, thin layer chromatography (TLC) analysis methods, and the high performance liquid chromatography (HPLC), 10 batches of wild agarwood are identified.[Result] The alcohol soluble extract content of 10 batches of wild agarwood is significantly higher than 10% regulated by Pharmacopoeia(2015). The color reaction of cultivated agarwood shows pale cherry red or cherry red, while the wild ones also show violet,light red and purple except cherry red. The color reaction can assist in the identification of wild agarwood samples.TLC is a simple and sample-saving method. The wild agarwood in TLC show clear fluorescent spots and good separation effect. The most of the wild samples can be identified by TLC. By comparing with similarity evaluation, peak area of 10 batches of wild agarwood, HPLC fingerprints and 9 common characteristic peaks are established. Moreover, the differences of wild and cultivated agarwood HPLC fingerprints, which can identify the wild agarwood effectively and accurately,are determined.[Conclusion] Based on the advantages of each method, this paper proposes HPLC common characteristic peaks with microscopic identification, color reaction, the alcohol soluble extract content and thin layer chromatography to identify the wild agarwood. The results will provide the reference for the establishment of quality evaluation system of agarwood.

Key words: wild agarwood, identification method, high performance liquid chromatography, fingerprint, common characteristic peak

中图分类号:

陈媛, 尚丽丽, 杨锦玲, 李改云, 殷亚方, 戴好富. 野生沉香的鉴别方法[J]. 林业科学, 2017, 53(9): 90-96.

Chen Yuan, Shang Lili, Yang Jinling, Li Gaiyun, Yin Yafang, Dai Haofu. The Exploration of Identification Method of Wild Agarwood[J]. Scientia Silvae Sinicae, 2017, 53(9): 90-96.

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