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林业科学 ›› 2009, Vol. 12 ›› Issue (9): 36-40.doi: 10.11707/j.1001-7488.20090907

• 论文 • 上一篇    下一篇

结实量对兴安落叶松和长白落叶松针叶内游离氨基酸含量的影响*

佟丽丽 严善春 金虎 石蕾 张健   

  1. 东北林业大学林木遗传育种与生物技术教育部重点实验室 哈尔滨 150040
  • 收稿日期:2008-06-16 修回日期:1900-01-01 出版日期:2009-09-25 发布日期:2009-09-25
  • 通讯作者: 严善春
  • 基金资助:
     

Effects of Cone Yield on Free Amino Acid Contents in the Needles of Larix gmelinii and L. olgensis

Tong Lili,Yan Shanchun,Jin Hu,Shi Lei,Zhang Jian   

  1. Key Laboratory of Forestry Tree Genetic Improvement and Biotechnology of Ministry of Education, Northeast Forestry University Harbin 150040
  • Received:2008-06-16 Revised:1900-01-01 Online:2009-09-25 Published:2009-09-25
  • Supported by:
     

摘要:

研究不同种类落叶松结实量与其抗性的关系,采用柱前衍生高效液相色谱法检测分析兴安落叶松和长白落叶松在不同结实等级情况下针叶内游离氨基酸含量变化。结果表明: 兴安和长白落叶松针叶内含有17种游离氨基酸,虽然苯丙氨酸在2种落叶松内均为优势氨基酸,但2种落叶松针叶内的多种与抗性相关的游离氨基酸含量存在较大差异; 兴安落叶松针叶内丙氨酸、精氨酸、谷氨酸、异亮氨酸、赖氨酸、苯丙氨酸和缬氨酸含量在不同结实等级间存在较大差异,长白落叶松针叶内天门冬氨酸和苯丙氨酸含量在不同结实等级间存在较大差异; 兴安落叶松针叶内天门冬氨酸含量显著小于其在长白落叶松针叶内含量; 当结实较少时,2种落叶松针叶内游离氨基酸总量,显著低于其他3个结实等级的游离氨基酸总量,结实较多时,针叶内游离氨基酸总量最高。说明结实量大小直接影响落叶松针叶内的资源分配,进而改变其对植食昆虫的适口性及抗性,种类间的差异对落叶松具有同样的影响。

 

关键词: 结实量, 兴安落叶松, 长白落叶松, 游离氨基酸, 高效液相色谱

Abstract:

Plants have evolved defenses against herbivores to improve their survival and reproduction. In order to study the relationship between resistance and cone yield in two larch species, contents of free amino acid in needles of Larix gmelinii and L. olgensis with different cone yield were measured by using high performance liquid chromatography(HPLC) with pre-column derivatization. The results showed that 17 kinds of free amino acids were detected out, and among them phenylalanine was the highest in both species. It was interesting to find there were significant differences in the several free amino acids related to resistance between the two species. Moreover, the contents of Alanine, Arginine, Glutamic acid, Isoleucine, Lysine, Phenylalanine and Valine in L. gmelinii were significantly different, and the contents of Asparagine and Phenylalanine in L. olgensis were significantly different, with different cone yield. The content of Asparagine in L. gmelinii was significantly less than in L. olgensis. The total content of free amino acids in leaves with the lowest cone yield was significantly lower than that in other grades of cone yield, and the total content of free amino acids increased with the increasing cone yield. It was concluded that the cone yield directly affected resource allocation in the needles of larch and further changed palatability and resistance of the herbivores. Furthermore, the difference of species was also important for resistance to their herbivores.

Key words: cone yield, Larix gmelinii, Larix olgensis, free amino acid, HPLC(high performance liquid chromatography)

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