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林业科学 ›› 2016, Vol. 52 ›› Issue (2): 57-66.doi: 10.11707/j.1001-7488.20160207

• 论文与研究报告 • 上一篇    下一篇

低磷胁迫下杉木幼苗生长特性与内源激素的关系

陈智裕1, 吴鹏飞1, 邹显花1, 汪攀1, 马静1,2, 马祥庆1   

  1. 1. 福建农林大学林学院 国家林业局杉木工程技术研究中心 福州 350002;
    2. 宁夏中卫市海原县水务局 海原 755200
  • 收稿日期:2015-10-19 修回日期:2015-11-10 出版日期:2016-02-25 发布日期:2016-03-25
  • 通讯作者: 吴鹏飞
  • 基金资助:
    国家自然科学基金项目(31370531,U1405211);福建省自然科学基金杰出青年科学基金项目(2014J06009);福建农林大学林学院青年教师科研基金项目(6112C035A)。

Relationship between Growth and Endogenous Hormones of Chinese Fir Seedlings under Low Phosphorus Stress

Chen Zhiyu1, Wu Pengfei1, Zou Xianhua1, Wang Pan1, Ma Jing1,2, Ma Xiangqing1   

  1. 1. Chinese Fir Engineering Technology Research Center of State Forestry Administration College of Forestry, Fujian Agriculture and Forestry University Fuzhou 350002;
    2. Haiyuan County Water Affairs Bureau in Zhongwei, Ningxia Haiyuan 755200
  • Received:2015-10-19 Revised:2015-11-10 Online:2016-02-25 Published:2016-03-25

摘要: [目的] 研究低磷环境中,不同磷利用效率杉木家系幼苗生长特性与其叶片和根尖内源激素含量变化的相关性,为进一步揭示磷高效利用杉木基因型对低磷胁迫响应策略的内在调控机制提供参考依据。[方法] 选择在缺磷环境下磷素利用效率较低的No.4与磷素利用效率较高的No.41杉木半同胞家系为研究对象,通过设计3个供磷水平(P0:不供磷,0 mmol·L-1 KH2PO4; P1:低度供磷,0.5 mmol·L-1 KH2PO4; P2:正常供磷,1.0 mmol·L-1 KH2PO4)的室内沙培模拟试验,分别测定2个家系幼苗在不同供磷水平下的生长特性以及叶片与根系内细胞分裂素(ZT)、赤霉素(GA3)、生长激素(IAA)和脱落酸(ABA)含量的变化。[结果] 1)不供磷条件下,41号家系杉木幼苗根长、根表面积和根平均直径的增量以及根冠比均明显高于4号家系(P<0.05)。2)低度供磷条件下,4号家系杉木幼苗叶片内ZT和IAA含量显著高于41号家系,而这2个家系杉木幼苗在不供磷条件下根系内ZT含量与正常供磷处理相比显著降低(P<0.05),IAA和ABA含量则显著增加(P<0.05),且41号家系杉木幼苗根系ABA含量显著高于4号家系的任意供磷水平(P<0.05)。3)正常供磷条件下,2个家系叶片、根系内源激素含量与幼苗生长特性指标均无显著相关(P> 0.05);低度供磷条件下,4号家系仅叶片IAA、GA3含量与根长增量呈显著负相关(P<0.05),而41号家系叶片ZT含量与根长、根表面积增量呈显著正相关(P<0.05),苗高增量与IAA呈显著正相关(P<0.05),与ABA呈显著负相关(P<0.05);不供磷条件下4号家系叶片IAA含量与苗高增量呈极显著正相关(P<0.01),根系ABA含量与根长、根表面积和根平均直径呈显著负相关(P<0.05),而41号家系仅有根平均直径与ZT和GA3呈一定的相关性(P<0.05);从根冠比来看,这2个家系在低度供磷和不供磷条件下,叶片内源激素含量与根冠比未达到显著相关(P> 0.05),但4号家系根冠比与根系ZT含量却成极显著正相关,与GA3含量呈极显著负相关(P<0.01),41号家系根冠比与根系ZT含量呈极显著负相关,与IAA含量呈极显著正相关(P<0.01)。[结论] 低水平供磷条件下,磷素利用效率较低的杉木家系(No.4)幼苗根系生长量明显低于磷素利用效率较高的杉木家系(No.41);不供磷条件下,不同磷素利用效率杉木家系(No.4和No.41)叶片和根系内ABA含量均显著增加,而根系仅有ZT含量显著降低,并分别与磷素利用效率较低家系(No.4)和磷素利用效率较高家系(No.41)的根冠比呈显著正、负相关性,这可能与不同磷利用效率杉木家系幼苗在低磷环境下根系皮层组织溶解并释放磷营养的能力有关,需进一步研究。

关键词: 杉木, 低磷环境, 根系形态, 植物激素, 调控作用

Abstract: We investigated the relationship between the growth characteristics and the change of endogenous hormone contents in needles and root tips of Chinese fir (Cunninghamia lanceolata) with different phosphorus (P) use efficiency under lower P condition, to provide a basis for further understanding of the mechanism of responding to the P stress.[Method] Two half-sib families of Chinese fir (No.4 and No.41, respectively with low and high P use efficiencies under no P supply condition) were treated with three different P levels (P1:no P supply, 0 mmol·L-1 KH2PO4; P2:low P supply, 0.5 mmol·L-1 KH2PO4; P3:normal P supply, 1.0 mmol·L-1 KH2PO4) and cultured in sand medium indoor to investigate the growth of seedlings under different P levels, and the contents of cytokinins (ZT), gibberellins (GA3), growth hormone (IAA) and abscisic acid (ABA) in needles and roots.[Result] 1) Under no P supply condition, the increments of the average length, surface area and diameter of roots, and the root to shoot ratio of family No.41 were significantly higher than those of family No.4 (P<0.05). 2) Under low P supply condition, the contents of ZT and IAA in the needles of family No.4 were significantly higher than those of family No.41; the content of ZT in roots of the two families were lower than those of normal P level (P<0.05), but both of IAA and ABA contents were significantly increased; the content of ABA in family No.41 seedlings roots was higher than that in family No.4 (P<0.05). 3) Under normal P supply condition, there was no significant correlation between the contents of endogenous hormones and the growth characteristics of the seedlings. Under low P supply condition, the contents of IAA and GA3 in family No.4 both were negatively correlated with the root length increment (P<0.05); for family No.41, the content of ZT in needles was positively correlated with root length increment, like as root surface area increment (P<0.05), and the content of IAA was positively correlated with the height increment, which was negatively correlated with ABA (P<0.05). Under no P supply condition, the content of IAA in needles of family No.4 was positively correlated with the height increment (P<0.01), whereas the content of ABA in the roots was negatively correlated with the increments of root length, root surface area and root diameter respectively (P<0.05); for family No.41, only the root diameter increment was significantly correlated with the content of ZT, like as GA3. The seedlings treated with low P and normal P levels displayed no significant correlation between the endogenous hormone contents in needles and the root to shoot ratio; the contents of ZT and GA3 in roots of family No.4 was positively and negatively correlated with the root to shoot ratio respectively, while the ZT content of family No.41 was negatively and positively correlated with the root to shoot ratio respectively (P<0.01).[Conclusion] Under low P supply condition, compared with family No.41 with higher P use efficiency, the root growth of family No.4 with lower P use efficiency was significantly inhibited. Under no P supply condition, the contents of ABA in needles and roots of the two families with different P use efficiencies (No.4 and No.41) were both significantly increased, but the content of ZT in roots significantly decreased; moreover, there was positive correlation between the ZT content and the root to shoot ratio in seedlings of family No.4, or negative correlation in seedlings of family No.41 which had a high P use efficiency. This is probably related to the capacities of different families with different P use efficiencies in diluting and releasing P nutrient by the aerenchyma of the roots under low P condition.

Key words: Cunninghamia lanceolata, low phosphorus condition, root morphology, plant hormones, regulatory role

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