外生菌根真菌在酸雨胁迫下对马尾松土壤微生物代谢功能的影响

doi:10.11707/j.1001-7488.20140714

摘要/Abstract

摘要：

利用BIOLOG方法研究酸雨胁迫下接种外生菌根真菌对马尾松幼苗土壤微生物的缓解作用。采用盆栽方法，设置3种不同pH 的模拟酸雨强度，分别设置彩色豆马勃接种和不接种的处理。结果表明：1）强酸胁迫可降低土壤微生物的活性、丰富度和多样性，降低土壤微生物对碳源的利用；2）弱酸的对照处理（pH5.6）下接种外生菌根真菌后可中和弱酸降水对土壤微生物的影响；3）强酸处理下接种外生菌根真菌能提高土壤微生物活性，提高丰富度和多样性，提高微生物对碳源的利用，且改变碳源利用结构。

关键词: 外生菌根真菌, 酸雨, 土壤微生物, 马尾松

Abstract:

This study aims to explore the effects of ectomycorrhizal colonization on soil microbial community and function of Pinus massoniana seedlings grown in acidified soils by using sole-carbon-source-utilization (BIOLOG). we set three levels of acid treatments, pH3.5, pH4.5 and pH5.6 (control), and applied two ectomycorrhizal treatments (inoculated Pisolithus tinctorius, non-inoculated) in a pot experiments. Masson pine seedlings were inoculated with P.tinctorius (ECM) for 210 days in acidified forest soil originating from Chongqing, Southwest China. The results showed that: 1) Compared with the control treatment (pH 5.6), high acid stress reduced the average vitality, soil microbial richness and diversity indices; 2) With the control treatment, ectomycorrhizal fungi counteracted the effects of mild acid on soil microbial community; 3) With high acid stress treatments, ectomycorrhizal fungi increased soil microbial activity, richness and diversity, and changed the utilization pattern of carbon source.

Key words: ectomycorrhizal fungi, acid rain, soil microbial community, masson pine

中图分类号:

S718.43

王琳, 陈展, 尚鹤. 外生菌根真菌在酸雨胁迫下对马尾松土壤微生物代谢功能的影响[J]. 林业科学, 2014, 50(7): 99-104.

Wang Lin, Chen Zhan, Shang He. Effects of Ectomycorrhizal Fungi(Pisolithus tinctorius) of Masson Pine (Pinus massoniana)on Soil Microbial Metabolic Function under Simulated Acid Rain[J]. Scientia Silvae Sinicae, 2014, 50(7): 99-104.

参考文献

陈承利. 2006. 重金属Cd、Pb、Hg污染对土壤微生物及其活性影响研究. 杭州:浙江大学硕士学位论文.
(Chen C L. 2006. Effects of cadmium,lead and mercury pollution on soil microorganisms and their activities. Hangzhou: MS thesis of Zhejiang University. [in Chinese] )
陈展,尚鹤. 2014. 接种外生菌根菌对模拟酸雨胁迫下马尾松营养元素的影响. 林业科学,50(1): 156-163.
(Chen Z, Shang H. 2014. Effects of ectomycorrhizal fungi on nutrient elements of Pinus massoniana seedlings under simulated acid rain. Scientia Silvae Sinicae, 50(1): 156-163. [in Chinese] )
陈展,王琳,尚鹤. 2013.接种彩色豆马勃对模拟酸沉降下马尾松幼苗生物量的影响. 生态学报, 33(20): 6526-6533.
(Chen Z, Wang L, Shang H. 2013. Effects of ectomycorrhizal fungi (tinctorius (Pers.) Coker & Couch)on the biomass of masson pine (Pinus massoniana) seedlings under simulated acid rain. Acta Ecologica Sinica, 33(20): 6526-6533. [in Chinese] )
陈展,王效科,段晓男,等. 2007.臭氧浓度升高对盆栽小麦根系和土壤微生物功能的影响. 生态学报, 27(5): 1803-1808.
(Chen Z, Wang X K, Duan X N, et al. 2007. Ozone effects on wheat root and soil microbial biomass and diversity. Acta Ecologica Sinica, 27(5): 1803-1808. [in Chinese] )
付晓萍, 田大伦.2006. 酸雨对植物的影响研究进展. 西北林学院学报, 21(4): 23-27.
(Fu X P, Tian D L. 2006. Research progress of the effect of acid rain on plant. Journal of Northwest Forestry University, 21(4): 23-27. [in Chinese] )
辜夕容,黄建国,梁国仕. 2005. 接种双色蜡蘑对马尾松幼苗生长、养分和抗铝性的影响. 林业科学,41(4): 199-203.
(Gu X R, Huang J G, Liang G S. 2005. Influences of laccaria bicolor on the growth, nutrient uptake and aluminum resistance of Pinus massoniana seedlings. Scientia Silvae Sinicae, 41(4): 199-203. [in Chinese] )
林慧萍.2005. 酸雨对陆生植物的影响机理. 福建林业科技,32(1): 60-64.
(Lin H P. 2005. Influencing mechanism of the acid rain on the terrestrial plant. Journal of Fujian Forestry Science and Technology, 32(1): 60-64. [in Chinese] )
林先贵,陈瑞蕊,胡君利. 2010. 土壤微生物资源管理、应用技术与学科展望. 生态学报,30(24): 7029-7037.
(Lin X G, Chen R R, Hu J L. 2010. The management and application of soil microbial resources and the perspectives of soil microbiology. Acta Ecologica Sinica, 30(24): 7029-7037. [in Chinese] )
林先贵,胡君利.2008. 土壤微生物多样性的科学内涵及其生态服务功能. 土壤学报,45(5): 892-900.
(Lin X G, Hu J L. 2008. Scientific connotation and ecological service function of soil microbial diversity. Acta Pedologica Sinica, 45(5): 892-900. [in Chinese] )
凌大炯,章家恩,欧阳颖. 2007. 酸雨对土壤生态系统影响的研究进展. 土壤,39(4): 514-521.
(Ling D J, Zhang J E, Ouyang Y. 2007. Advancements in research on impact of acid rain on soil ecosystem: a review. Soils, 39(4): 514-521. [in Chinese] )
谈建康,孔繁翔. 2005. 酸沉降和铝对马尾松菌根共生体碳代谢影响. 林业科学,41(6): 23-27.
(Tan J K, Kong F X. 2005. Effects of acid precipitation and Al on Carbon metabolism in mycorrhizal symbionts of Pinus massoniana. Scientia Silvae Sinicae, 41(6): 23-27. [in Chinese] )
王玲,黄世文,刘连盟,等. 2009. 三种研究农田土壤微生物多样性方法的比较. 科技通报,25(5): 588-592.
(Wang L, Huang S W, Liu L M, et al. 2009. Comparation of three methods for studying microbial diversity of farmland soil. Bulletin of Science and Technology, 25(5): 588-592. [in Chinese] )
杨宗慧. 2002. 我国酸雨状况和对策.云南环境科学, 21(1): 25-26.
(Yang Z H. 2002. Acid rain in china and its countermeasures. Yunnan Environmental Science, 21(1): 25-26. [in Chinese] )
张德明,陈章和. 1998. 白云山土壤微生物的季节变化及其对环境污染的反应. 生态科学,17(1): 40-45.
(Zhang D M, Chen Z H. 1998. Seasonal variation of soil microbes and its reaction to environment pollution. Ecologic Science, 17(1): 40-45. [in Chinese] )
张海涵. 2008. 林木菌根真菌与土壤微生物相互关系的研究. 杨凌:西北农林科技大学硕士学位论文.
(Zhang H H. 2008. The relationship between mycorrhizal fungi and soil microbial under tree species. Yangling: MS thesis of Northwest A&F University. [in Chinese] )
张慰,陈展,邓仕槐,等. 2012. 外生菌根缓解酸雨胁迫的机理研究进展. 生态学杂志, 31(1): 200-206.
(Zhang W, Chen Z, Deng S H, et al. 2012. Ectomycorrhizae-induced mitigation mechanisms against the damages of acid rain on plants:A review. Chinese Journal of Ecology, 31(1): 200-206. [in Chinese] )
Agarwal P, Sah P. 2009. Ecological importance of ectomycorrhizae in world forest ecosystems. Nature and Science, 7 (2): 107-116.
Baldrian P, Kolaîík M, Štursová M, et al. 2012. Active and total microbial communities in forest soil are largely different and highly stratified during decomposition. The ISME Journal, 6(2): 248-258.
Benizri E, Amiaud B. 2005. Relationship between plants and soil microbial communities in fertilized grasslands. Soil Biology & Biochemistry, 37(11): 2055-2064.
Bossio D D,Scow K M. 1995. Impact of carbon and flooding on the metabolic diversity of mi crobial communities in soils.Appllied Environmental Microbiology, 61: 4043-4050.
Bossio D A, Girvan M S, Verchot L, et al. 2005. Soil microbial community response to land use change in an agricultural landscape of Western Kenya. Microbial Ecology, 49 (1): 50-62.
Chen Z, Wang X K, Feng Z Z, et al. 2009. Impact of elevated O₃ on soil microbial community function under wheat crop. Water, Air and Soil Pollution, 198 (1-4): 189-198.
Chen Z, Wang X K, Yao F F, et al. 2010. Elevated ozone changed soil microbial community in a rice paddy. Soil Science and Society of American Journal, 74(3): 829-837.
Copy C C, Dianarn R N,Steven K S, et al. 2001.Increases in soft respiralion following labile carbon additions linked Biogeochemistry, 82:229-240.
Garland J L, Mills A L. 1991. Classification and characterization of heterotrophic microbial communities on the basis of patterns of community-level sole-carbon-source-utilization. App1lied Environmental Microbiology, 57 (8): 2351-2359.
Haack S K, Garchow H M, Klug J, et al. 1995.Analysis of factors affecting the accuracy, reproducibility, and interpretation of microbial community carbon source utilization patterns. Applied and Environmental Microbiology, 61(4): 1458-1468.
Pennanen T, Fritze H, Vanhala P, et al. 1998a. Structure of a microbial community in soil after prolonged addition of low levels of simulated acid rain. Applied and Environmental microbiology, 64 (6): 2173-2180.
Pennanen T, Perkimki J, Kiikkil O, et al. 1998b. Prolonged, simulated acid rain and heavy metal deposition: Separated and combined effects on forest soil microbial community structure. FEMS Microbiology Ecology, 27 (3): 291-300.
Petra M, Karen B. 2003. Changes in bacterial community structure induced by mycorrhizalcolonisation in split-root maize. Plant and Soil, 251 (2): 279-289.
Rogers B F, Tate III R L. 2001. Temporal analysis of the soil microbial community along a topo sequence in Pineland soils. Soil Biology and Biochemistry, 33 (10): 1389-1401.
Van Hees P A W, Jones D L, Jentschke G, et al. 2005.Organic acid concentrations in soil solution: Effects of young coniferous trees and ectomycorrhizal fungi. Soil Biology and Biochemistry, 37(4): 7712-7761.
Wang G H, Jin J, Chen X L, et al. 2007. Biomass and catabolic diversity of microbial communities with long-term restoration, bare fallow and cropping history in Chinese Mollisols. Plant Soil Environemnt, 53 (4): 177-185.
Xue D, Yao H Y, Ge D Y, et al. 2008. Soil microbial community structure in diverse land use systems: A comparative study using Biology, DGGE, and PLFA analyses. Pedosphere, 18 (5): 653-663.
Zabinski C A, Gannon J E. 1997. Effects of recreational impacts on soil microbial communities. Environmental Manage, 21(2): 233-238.
Zak J C,Willig M R,Moorhead D L,et al.1994. Functional diversity of microbial communities: A quantitative approach. Soil Biology & Biochemistry, 26 (9): 1101-1108.

[1]	邓秀秀, 肖文发, 曾立雄, 雷蕾, 施征. 马尾松幼苗光合产物的运输与分配特征[J]. 林业科学, 2019, 55(7): 27-34.
[2]	金国庆, 张振, 余启新, 冯随起, 丰忠平, 赵世荣, 周志春. 马尾松2个世代种子园6年生家系生长的遗传变异与增益比较[J]. 林业科学, 2019, 55(7): 57-67.
[3]	丁令智, 满秀玲, 肖瑞晗, 蔡体久. 寒温带森林根际土壤微生物量碳氮含量生长季内动态变化[J]. 林业科学, 2019, 55(7): 178-186.
[4]	李一凡, 王玉杰, 王彬, 李通. 西南酸雨区重庆缙云山常绿阔叶林土壤氮矿化特征[J]. 林业科学, 2019, 55(6): 1-12.
[5]	王超群, 焦如珍, 董玉红, 厚凌宇, 赵京京, 赵世荣. 不同林龄杉木人工林土壤微生物群落代谢功能差异[J]. 林业科学, 2019, 55(5): 36-45.
[6]	李亚藏, 冯仲科. 气候敏感的马尾松生物量相容性方程系统研建[J]. 林业科学, 2019, 55(5): 65-73.
[7]	王好运, 吴峰, 朱小坤, 谢维斌. 叶型对马尾松幼苗生长及叶绿素荧光特征的影响[J]. 林业科学, 2019, 55(3): 183-192.
[8]	陈金磊, 方晰, 辜翔, 李雷达, 刘兆丹, 王留芳, 张仕吉. 中亚热带2种森林群落组成、结构及区系特征[J]. 林业科学, 2019, 55(2): 159-172.
[9]	陈坦, 张振, 楚秀丽, 金国庆, 周志春, 丰忠平. 马尾松二代无性系种子园的花期同步性[J]. 林业科学, 2019, 55(1): 146-156.
[10]	李伟成, 盛海燕, 蒋跃平, 温星. 基塘系统不同竹林土壤CO₂通量特征及其影响因子[J]. 林业科学, 2018, 54(8): 13-22.
[11]	罗晓蔓, 丁贵杰, 王艺. 菌根化马尾松苗木根际土壤浸提物有效成份及其对种子萌发的影响[J]. 林业科学, 2018, 54(8): 32-38.
[12]	姚虹宇, 刘亚敏, 张盛楠, 刘玉民, 周文颖, 王针针. 外源柠檬酸对铝胁迫下马尾松生理特性的影响[J]. 林业科学, 2018, 54(7): 155-164.
[13]	魏永成, 刘青华, 周志春, 徐六一, 陈雪莲, 郝焰平. 不同抗性马尾松接种松材线虫后针叶内化学信号物质的变化[J]. 林业科学, 2018, 54(2): 119-125.
[14]	辜夕容, 倪亚兰, 江亚男, 贾豪. 接种双色蜡蘑对酸性铝胁迫下马尾松幼苗生长、养分和铝吸收与分布的影响[J]. 林业科学, 2018, 54(2): 170-178.
[15]	尹焕焕, 刘青华, 周志春, 余启新, 丰忠平. 马尾松产脂性状与生长性状的无性系变异及相关性[J]. 林业科学, 2018, 54(12): 82-91.