关键词: 木材白腐菌, 酚酸, 高效液相色谱分析

Abstract:

Because different woodrotting fungi have different wood degrading mechanisms to the same ligneous substrate, the composition and contents of phenolic acids of the same wood species degraded by different woodrotting fungi can exhibit different changes. In order to get more further study on wood biodegrading mechanism with david poplar wood degraded by wood whiterot fungi, different phenolic acids and their contents of david poplar wood degraded by 6 species of wood whiterot fungi living on broadleaf trees, i.e. Funalia gallica, Lenzites tricolor, Phellinus igniarius, Polyporellus brumalis, Pseudotrametes gibbosa, and Pycnoporus sanguineus were analyzed. HPLC method was used to measure the contents of dissociated phenolic acids of primitive wood sample and wood samples degraded by 6 species of fungi on 120 d, respectively, HPLC chromatograms of different samples were gained. A little powders shaved from the very surface of primitive wood sample and wood samples degraded by 6 species of fungi on 120 d were milled under dryness, vibrated by ultrasonic in petroleum ether and circumfluenced in methanol, then various phenolic acids in the same sample were separated from higher polarity to lower polarity in a reverse phenyl column, mg contents of various phenolic acids in each of 100 g sample could be calculated by the chromatogram area of various dissociated phenolic acids in different samples. Results showed that the kinds and contents of phenolic acids in primitive david poplar wood and david poplar wood degraded by 6 species of wood whiterot fungi on 120 d were different, the total contents of 9 kinds of dissociated phenolic acids in wood samples degraded by Pseudotrametes gibbosa,Lenzites tricolor, Pycnoporus sanguinus, and Polyporellus brumalis were higher than those degraded by Funalia gallica and Phellinus igniarius, indicating that the former 4 whiterot fungi with higher wood degrading ability gained higher contents of phenolic acids. But the total content of 9 kinds of dissociated phenolic acids in one sample was not completely same with wood and lignin degrading percent, suggesting that other dissociated phenolic acids or aromatic ring dimmer, trimer oligomer and heterocyclic compounds might be produced besides 9 kinds of dissociated phenolic acids tested, all of the aromatic compounds contributed to lignin degrading percent, 9 kinds of dissociated phenolic acids tested only was not enough to explain the lignin degrading ability, all other aromatic compounds except 9 kinds of dissociated phenolic acids should also be tested later. The kind and content of phenolic acids tested of each whiterot fungus were different from those of each other, indicating that different white rot fungi had different degrading pathway and intermediate degrading compounds to the same ligneous substrate. In addition, a little phenolic acids were in primitive wood sample which could be degradated and transformed into other kinds of compounds in the process of biodegradation, it would be further determined whether the primitive intrinsic phenolic acids could be transformed into other kinds of phenolic acids.

Key words: wood whiterot fungi, phenolic acids, HPLC analysis