欢迎访问林业科学,今天是

林业科学 ›› 2005, Vol. 41 ›› Issue (2): 178-181.doi: 10.11707/j.1001-7488.20050231

• 研究简报 • 上一篇    下一篇

银杏胚轴、子叶诱导胚状体发生及成苗的研究

郭长禄1,2 陈力耕1 何新华1,3 Syed Asgar Tori1 袁海英1   

  1. 1.浙江大学农业部园艺植物生长发育与生物技术重点开放实验室,杭州310029;2.哈尔滨工业大学海洋学院威海264209;3.广西大学农学院南宁530005
  • 收稿日期:2003-10-31 修回日期:1900-01-01 出版日期:2005-03-25 发布日期:2005-03-25

Study on Somatic Embryogenesis of Embryonic Axis and Cotyledon in Ginkgo biloba and Plantlet Formation

Guo Changlu1,2,Chen Ligeng1,He Xinhua1,3,Syed Asgar Tori1,Yuan Haiying1   

  1. 1.Key Laboratory of Ministry of Agriculture for Growth Development and Biotechnology of Horticultural Plant, Zhejiang University Hangzhou 310029; 2.School of the Ocean,Harbin institute of technology Weihai 264209;3.Agricultural College of Guangxi University Nanning 530005
  • Received:2003-10-31 Revised:1900-01-01 Online:2005-03-25 Published:2005-03-25

关键词: 银杏, 胚轴, 子叶, 胚状体发生, 植株再生

Abstract:

Embryonic axis and cotyledon of immature and mature embryos were induced as explants for embryogenesis in Ginkgo biloba. Results showed somatic embryos could be induced only through embryonic axis and cotyledon of immature embryos. MK + NAA 1 mg·L-1 + 6-BA 1 mg·L-1 was the best medium for somatic embryogenesis from embryonic axis, and somatic embryogenesis rate was 45.28%. MK + NAA 1.5 mg·L-1 + 6-BA 1 mg·L-1 was the best medium for somatic embryogenesis from cotyledon, and somatic embryogenesis rate was 12.9%. The growth and development of somatic embryos were promoted by adding 10% coconut milk into MK, and 34.48% somatic embryos could develop into plants.

Key words: Ginkgo biloba, embryonic axis, cotyledon, somatic embryogenesis, plant generation