转BpGLK裂叶桦生长变异、根际土壤酶活性及微生物群落组成

doi:10.11707/j.1001-7488.20221203

Abstract

Abstract:

Objective: This study aims to provide theoretical support for the evaluation of the subsequent environmental security as well as the industrialization of transgenic BpGLK Betula pendula 'Dplecprlicp', by studying the soil physicochemical properties, the enzyme activity and the community composition of the rhizosphere soil microorganism of transgenic BpGLK B. pendula 'Dplecprlicp'. Method: Two-year-old BpGLK transformed B.pendula 'Dplecprlicp' (RE1-RE5) were used to study the stability of exogenous genes. The growth characteristics, physicochemical properties and enzyme activity of rhizosphere soil were determined. High-throughput sequencing technology was used to analyze the community characteristics of soil bacteria and fungi. Result: The Bar gene and BpGLK interference fragment were still integrated in the genome of transgenic B. pendula 'Dplecprlicp' strains. As for the growth traits, RE strains were relatively higher or significantly higher than WT strain (RE3 excepted). The soil pH, contents of organic carbon, total nitrogen and available nitrogen of RE strains were lower or significantly lower than those of WT strain. The contents of available phosphorus, total potassium and available potassium in RE strains were significantly higher than those in WT strain (except RE1) (P < 0.05). RE strains had lower or significantly lower soil urease and sucrase activities than WT strain, while RE strains had significantly higher neutral protease activity than WT strain. The analysis of the community richness and diversity indicated that there was no significant difference in the bacteria community between RE strains and WT strain. The fungi richness of RE strains was higher or significantly higher than that of WT strain. However, there was no significant difference in fungi diversity between RE strains and WT strain. As for Saccharimonadales, Acidothermus and other 8 genera of bacteria, RE strains were significantly higher than WT strain, while Candidatus_ Soliactor and other 4 genera were significantly lower than WT strain. In the fungal community, Tomentella was the dominant group in the rhizosphere soil of RE strains. Conclusion: The growth of two-year-old transgenic BpGLK B.pendula 'Delecprlicp' outperforms that of wild-type strain. No adverse effects of transgenic activities on physicochemical properties, enzyme activity and microbial community composition of rhizosphere soil have been found in this experiment.

Key words: birch, transgenic, rhizosphere, soil enzyme activity, soil microbial community

CLC Number:

S792.15

Li Cao,Yang Wang,Yunli Yang,Yu Zheng,Wei Wang,Guifeng Liu,Jing Jiang. Growth Variation, Rhizosphere Soil Enzyme Activity and Microbial Community Composition of Transgenic BpGLK Betula pendula 'Dplecprlicp'[J]. Scientia Silvae Sinicae, 2022, 58(12): 21-31.

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引物名称 Primer name	引物序列(5′-3′) Primer sequence	扩增片段长度 Amplified fragment length/bp
BpGLK_RNAi_Cis_F	CATGCCATGGGCACAGAAGGTTTGTGCAAG	414
BpGLK_RNAi_Cis_R	TTGGCGCGCCCCATACATCTGCCTTCTCTGG	414
BpGLK_RNAi_Anti_F	GCTCTAGAGCACAGAAGGTTTGTGCAAG	429
BpGLK_RNAi_Anti_R	CGCGGATCCCCATACATCTGCCTTCTCTGG	429
Bar-F	TTAGATCTCGGTGACGGGCA	500
Bar-R	CGGTCTGCACCATCGTCAAC	500

株系 Strain	pH值 pH value	有机碳 Organic carbon/(g·kg^-1)	全氮 Total nitrogen/(g·kg^-1)	全磷 Total phosphorus/(g·kg^-1)	全钾 Total potassium/(g·kg^-1)	速效氮 Available nitrogen/(mg·kg^-1)	速效磷 Available phosphorus/(mg·kg^-1)	速效钾 Available potassium/(mg·kg^-1)
WT	5.06±0.05a	76.80±1.15a	4.05±0.06a	0.73±0.08a	22.10±0.06c	364.70±3.70a	26.30±4.91c	99.67±0.88b
RE1	4.93±0.09ab	67.83±0.69c	3.70±0.14b	0.66±0.03a	24.40±0.55b	338.63±10.34b	58.50±3.89b	79.33±8.41c
RE2	4.70±0.12b	66.90±0.15c	3.69±0.07b	0.72±0.08a	26.23±0.67a	318.00±0.23c	82.20±8.66a	122.67±3.76a
RE3	4.65±0.08b	70.77±0.97b	3.73±0.04b	0.72±0.01a	24.37±0.20b	361.35±1.36a	83.50±4.04a	111.67±6.89ab

株系 Strain	脲酶活性 Urease activity/(μg·d^-1g^-1)	蔗糖酶活性 Invertase activity/(mg·d^-1g^-1)	中性蛋白酶活性 Neutral protease activity/(mg·d^-1g^-1)
WT	658.73±17.66a	32.38±1.09a	5.29±0.04c
RE1	455.97±12.24b	11.63±0.74c	7.65±0.48a
RE2	459.46±18.35b	29.92±0.42a	6.38±0.19b
RE3	433.83±14.02b	18.75±1.70b	7.07±0.40ab

微生物 Microbial	样品 Samples	检测到的物种数 Observed_species	Chao1指数 Chao1 index	香农指数 Shannon index	Simpson指数 Simpson index	测序深度指数 Coverage
细菌 Bacteria	WT	6 217.03±192.10a	7 221.13±307.51a	11.20±0.07a	0.999±0.000a	0.944±0.004a
	RE1	5 705.98±324.68a	6 862.75±589.74a	10.93±0.09a	0.998±0.000a	0.945±0.007a
	RE2	6 091.20±461.23a	7 081.53±634.46a	11.09±0.19a	0.998±0.000a	0.945±0.007a
	RE3	5 503.93±665.56a	6 459.25±931.47a	10.86±0.26a	0.998±0.000a	0.950±0.010a
真菌 Fungi	WT	206.95±19.59b	208.45±20.15b	3.22±0.28ab	0.790±0.044a	0.999±0.000a
	RE1	339.10±46.95a	341.53±46.70a	4.00±0.29a	0.841±0.028a	0.998±0.000a
	RE2	250.50±17.37ab	252.75±17.42ab	3.17±0.25b	0.748±0.036a	0.998±0.000a
	RE3	209.43±22.64b	211.02±23.05b	3.39±0.12ab	0.827±0.026a	0.998±0.000a

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Growth Variation, Rhizosphere Soil Enzyme Activity and Microbial Community Composition of Transgenic BpGLK Betula pendula 'Dplecprlicp'

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