Scientia Silvae Sinicae ›› 2021, Vol. 57 ›› Issue (4): 163-172.doi: 10.11707/j.1001-7488.20210417
• Scientific notes • Previous Articles Next Articles
Yun Xie,Fangyun Guo,Lihua Chen,Bing Cao*
Received:
2020-01-06
Online:
2021-04-01
Published:
2021-05-21
Contact:
Bing Cao
CLC Number:
Yun Xie,Fangyun Guo,Lihua Chen,Bing Cao. Effects of Elevated CO2 Concentration on Soil Microbial Functional Diversity and Carbon Source Utilization Characteristics in the Root Zone of Lycium barbarum[J]. Scientia Silvae Sinicae, 2021, 57(4): 163-172.
Table 1
Soil physical and chemical properties of samples"
处理Treatment | pH | 全碳Total C /(g·kg-1) | 全磷Total P/(g·kg-1) | 全氮Total N/(g·kg-1) | 速效磷Available P/(mg·kg-1) | 速效钾Available K/(mg·kg-1) | 碱解氮Alkali-hydrolyzable N/(mg·kg-1) | 有机质Organic matter/(g·kg-1) |
CK | 8.647±0.064a | 3.050±0.095a | 0.840±0.040a | 0.263±0.050a | 57.610±0.915a | 87.837±0.333a | 26.133±0.817a | 15.973±1.216a |
TR1 | 8.643±0.035a | 3.000±0.040a | 0.830±0.029a | 0.278±0.023a | 56.687±0.704a | 86.053±1.315a | 25.433±0.650a | 15.577±0.803a |
TR2 | 8.737±0.029a | 3.007±0.158a | 0.877±0.023a | 0.327±0.009a | 57.723±0.943a | 87.477±1.177a | 24.850±0.202a | 17.350± 0.689a |
Table 2
Functional diversity index of soil microbes under elevated CO2"
处理时间 Treatment time/d | 处理 Treatment | 平均颜色变化率 AWCD | 物种丰富度指数 Shannon index(H′) | 优势度指数 Simpson index(D) | 均匀度指数 McIntosh index(U) | 均一度指数 (E) |
30 | CK | 1.014±0.081c | 2.573±0.218b | 0.807±0.076b | 6.472±0.489b | 0.813±0.070b |
TR1 | 1.473±0.034b | 3.169±0.015a | 0.954±0.005ab | 11.112±0.717a | 0.982±0.002ab | |
TR2 | 1.673±0.034a | 3.469±0.108a | 0.970±0.013a | 11.447±1.212a | 0.921±0.032b | |
60 | CK | 1.159±0.124b | 2.864±0.089c | 0.926±0.034a | 7.615±0.475b | 0.796±0.053b |
TR1 | 1.408±0.043ab | 3.220±0.008b | 0.956±0.007a | 13.160±0.778a | 0.988±0.002a | |
TR2 | 1.649±0.040a | 3.558±0.044a | 0.962±0.002a | 11.516±1.132a | 0.930±0.025a | |
90 | CK | 1.020±0.187b | 2.844±0.086b | 0.949±0.067a | 7.945±0.571b | 0.870±0.018b |
TR1 | 1.284±0.116b | 3.083±0.098b | 0.949±0.007a | 9.016±0.942b | 0.871±0.015b | |
TR2 | 1.821±0.065a | 3.424±0.072a | 0.967±0.008a | 12.782±1.095a | 0.955±0.032a | |
120 | CK | 1.085±0.107b | 2.697±0.184b | 0.720±0.032b | 6.922±1.128b | 0.748±0.086b |
TR1 | 1.360±0.014ab | 3.172±0.010a | 0.954±0.006a | 9.138±0.197ab | 0.882±0.002ab | |
TR2 | 1.590±0.098a | 3.218±0.025a | 0.957±0.001a | 11.256±1.157a | 0.939±0.025a |
Table 3
Carbon sources loading factors of PC1 and PC2 of PCA in the root zone of wolfberry under elevated CO2"
碳源类型 Category of carbon source | 代称 Synonym | 底物 Substrate | 30 d- PC1 | 30 d- PC2 | 60 d- PC1 | 60 d- PC2 | 90 d- PC1 | 90 d- PC2 | 120 d- PC1 | 120 d- PC2 |
糖类Carbohydrate | A2 | β-甲基-D-葡萄糖苷β-methyl-D-glucoside | 0.149 | 0.243 | — | 0.160 | 0.218 | 0.053 | 0.236 | 0.037 |
B2 | D-木糖D-xylose | 0.139 | 0.260 | 0.222 | — | — | — | — | — | |
E1 | α-环状糊精α-cyclodextrin | — | 0.028 | 0.125 | 0.248 | 0.083 | — | — | — | |
F1 | 肝糖Glycogen | — | — | 0.218 | — | 0.191 | — | 0.229 | — | |
G1 | D-纤维二糖D-cellobiose | 0.210 | — | — | 0.049 | 0.202 | 0.126 | — | 0.107 | |
H1 | α-D-乳糖α-D-lactose | — | 0.087 | — | 0.085 | 0.221 | 0.012 | 0.160 | 0.203 | |
G2 | 葡萄糖-1-磷酸盐Glucose-1-phosphate | 0.193 | 0.137 | 0.006 | — | 0.213 | 0.081 | 0.190 | 0.165 | |
氨基酸类Amino acid | A4 | L-精氨酸L-arginine | 0.197 | — | 0.013 | 0.298 | 0.130 | 0.250 | 0.097 | — |
B4 | L-天冬酰胺酸L-asparaginic acid | 0.210 | — | — | — | 0.186 | — | 0.202 | 0.144 | |
C4 | L-苯基丙氨酸L-phenylalanine | 0.205 | 0.073 | 0.083 | — | 0.207 | — | 0.237 | 0.029 | |
D4 | L-丝氨酸L-serine | 0.201 | — | — | — | 0.186 | 0.166 | 0.155 | 0.207 | |
E4 | L-苏氨酸L-threonine | 0.208 | — | — | — | 0.205 | — | 0.213 | 0.122 | |
F4 | 甘胺酰-L-谷氨酸Glycyl-L-glutamic acid | 0.208 | — | — | 0.063 | 0.206 | — | 0.227 | 0.081 | |
酯类Ester | B1 | 丙酮酸甲酯Methyl pyruvate | 0.070 | 0.326 | 0.224 | — | 0.218 | — | 0.134 | — |
C1 | 吐温-40 Tween-40 | 0.098 | 0.305 | 0.100 | 0.268 | 0.146 | 0.231 | 0.041 | — | |
D1 | 吐温-80 Tween-80 | — | 0.321 | 0.212 | 0.101 | 0.170 | 0.197 | 0.166 | — | |
A3 | D-半乳糖酸γ内酯D-galactoside γ lactone | 0.209 | 0.041 | 0.211 | 0.102 | 0.209 | — | 0.066 | — | |
醇类Alcohol | C2 | I-赤藻糖醇I-erythritol | 0.076 | 0.322 | — | 0.130 | 0.217 | 0.059 | 0.234 | — |
D2 | D-甘露醇D-mannitol | 0.195 | — | — | — | — | 0.008 | 0.068 | 0.263 | |
H2 | D, L-a-甘油D, L-a-glycerin | 0.103 | 0.301 | 0.060 | 0.288 | 0.214 | 0.077 | 0.110 | 0.243 | |
胺类Amine | G4 | 苯乙基胺Phenethylamine | 0.066 | — | 0.176 | — | — | 0.304 | 0.100 | 0.249 |
H4 | 腐胺Putrescine | 0.152 | — | — | 0.265 | 0.118 | 0.260 | — | — | |
E2 | N-乙酰基-D-葡萄胺N-acetyl-D-glucosamine | 0.206 | 0.068 | — | — | 0.117 | — | — | 0.219 | |
酸类Acid | B3 | D-半乳糖醛酸D-galacturonic acid | 0.210 | — | — | — | 0.052 | 0.299 | 0.124 | 0.234 |
C3 | 2-羟基苯甲酸2-hydroxybenzoic acid | 0.171 | — | 0.183 | 0.173 | — | 0.274 | — | — | |
D3 | 4-羟基苯甲酸4-hydroxybenzoic acid | 0.209 | 0.040 | 0.163 | — | 0.202 | — | 0.219 | — | |
E3 | γ-羟基丁酸γ-hydroxybutyric acid | 0.207 | — | 0.225 | 0.001 | 0.127 | 0.252 | 0.057 | — | |
F3 | 衣康酸Itaconic acid | 0.210 | — | — | 0.074 | 0.215 | 0.071 | — | 0.207 | |
G3 | α-丁酮酸α-butanone acid | — | 0.088 | — | 0.030 | 0.158 | — | 0.232 | — | |
H3 | D-苹果酸D-malic acid | — | 0.099 | 0.216 | — | 0.216 | — | — | 0.174 | |
F2 | D-氨基葡萄糖酸D-glucosaminic acid | 0.210 | 0.019 | 0.224 | 0.019 | 0.218 | — | 0.238 | — |
曹宏杰, 王立民, 徐明怡, 等. 五大连池新期火山熔岩台地不同植被类型土壤微生物群落功能多样性. 生态学报, 2019, 39 (21): 1- 11. | |
Cao H J , Wang L M , Xu M Y , et al. Effect of vegetation type on the diversity of soil microbial communities at the new stage Volcanic Lava Platform, Wudalianchi area, Northeast China. Acta Ecologica Sinica, 2019, 39 (21): 1- 11. | |
党雯, 郜春花, 张强, 等. Biolog法测定土壤微生物群落功能多样性预处理方法的筛选. 中国农学通报, 2015, 16 (10): 2247- 2251, 2255. | |
Dang W , Gao C H , Zhang Q , et al. Screening of preprocessing methods of biolog for soil microbial community functional diversity. Chinese Agricultural Science Bulletin, 2015, 16 (10): 2247- 2251, 2255. | |
邓娇娇, 朱文旭, 周永斌, 等. 不同土地利用模式对辽东山区土壤微生物群落多样性的影响. 应用生态学报, 2018, 29 (7): 2269- 2276. | |
Deng J J , Zhu W X , Zhou Y B , et al. Effects of different land use patterns on soil microbial community diversity in montane region of eastern Liaoning Province, China. Chinese Journal of Applied Ecology, 2018, 29 (7): 2269- 2276. | |
房蕊, 鲁彩艳, 史奕. CO2和O3浓度升高对土壤碳水化合物累积分布特征的影响. 农业环境科学学报, 2010, 29 (S1): 285- 288. | |
Fang R , Lu C Y , Shi Y . Effects of elevated CO2 and O3 on accumulation and distribution characteristics of soil carbohydrate. Journal of Agro-Environment Science, 2010, 29 (S1): 285- 288. | |
郭芳芸, 哈蓉, 马亚平, 等. CO2浓度升高对宁夏枸杞苗木光合特性及生物量分配影响. 西北植物学报, 2019, 39 (2): 302- 309. | |
Guo F Y , Ha R , Ma Y P , et al. Effects of elevated CO2 concentration on photosynthetic characteristics and biomass allocation of Lycium barbarum seedlings. Acta Botanica Boreali-Occidentalia Sinica, 2019, 39 (2): 302- 309. | |
哈蓉, 马亚平, 曹兵, 等. 模拟CO2浓度升高对宁夏枸杞营养生长与果实品质的影响. 林业科学, 2019, 55 (6): 28- 36. | |
Ha R , Ma Y P , Cao B , et al. Effects of simulated elevated CO2 concentration on vegetative growth and fruit quality in Lycium barbarum. Scientia Silvae Sinicae, 2019, 55 (6): 28- 36. | |
贾夏, 董岁明, 周春娟. 微生物生态研究中Biolog Eco微平板培养时间对分析结果的影响. 应用基础与工程科学学报, 2013, 21 (1): 10- 19.
doi: 10.3969/j.issn.1005-0930.2013.01.002 |
|
Jia X , Dong S M , Zhou C J . Effects of Biolog Eco-plates incubation time on analysis results in microbial ecology researches. Journal of Basic Science and Engineering, 2013, 21 (1): 10- 19. | |
雷霆, 周桔. 土壤微生物多样性影响因素及研究方法的现状与展望. 生物多样性, 2007, 15 (3): 306- 311.
doi: 10.3321/j.issn:1005-0094.2007.03.012 |
|
Lei T , Zhou J . Review and prospects on methodology and affecting factors of soil microbial diversity. Biodiversity Science, 2007, 15 (3): 306- 311.
doi: 10.1360/biodiv.070069 |
|
刘赛, 杨孟可, 李叶林, 等. 不同产地枸杞叶片多糖、总黄酮和总酚含量差异比较分析. 中国中药杂志, 2019, 44 (9): 1774- 1780. | |
Liu S , Yang M K , Li Y L , et al. Variance analysis on polysaccharide, total flavonoids and total phenols of Lycium barbarum leaves from different production areas. Chinese Journal of Chinese Materia Medica, 2019, 44 (9): 1774- 1780. | |
马海军, 张晓荣, 陈虹羽. 不同产区枸杞品质比较研究. 西北农业学报, 2015, 24 (8): 153- 156, 90. | |
Ma H J , Zhang X R , Chen H Y . Quality of Lycium barbarum L. in different regions. Acta Agricultural Boreali-Occidentalis Sinica, 2015, 24 (8): 153- 156. | |
马红亮, 朱建国, 谢祖彬, 等. 自由自然环境大气CO2浓度升高对稻田CH4排放的影响研究. 农业环境科学学报, 2010, 29 (6): 1217- 1224. | |
Ma H L , Zhu J G , Xie Z B , et al. The effects of elevated atmospheric [CO2] on emission of CH4 from rice paddy field. Journal of Agro-Environment Science, 2010, 29 (6): 1217- 1224. | |
马亚平, 王乃功, 贾昊, 等. 改进式开顶气室模拟CO2浓度控制系统性能分析. 地球环境学报, 2019, 10 (3): 307- 315. | |
Ma Y P , Wang N G , Jia H , et al. Evaluation of a modified open-top chamber simulation system on the study of elevated CO2 concentration effects. Journal of Earth Environment, 2019, 10 (3): 307- 315. | |
牛艳. 宁夏枸杞有效成分及其与生态因子关系的研究. 银川: 宁夏大学硕士学位论文, 2005. | |
Niu Y . Studies on main contents of Lycium barbarum L. and relation on environment factors. Yinchuan: MS thesis of Ningxia University, 2005. | |
钱明媚. 碳稳定性同位素示踪免耕土壤微生物多样性研究. 南京: 南京农业大学硕士学位论文, 2015. | |
Qian M M . Diversities of microbes in no-tillace soils revealed by stable isotope probing. Nanjing: MS thesis of Nanjing Agricultural University, 2015. | |
王超群, 焦如珍, 董玉红, 等. 不同林龄杉木人工林土壤微生物群落代谢功能差异. 林业科学, 2019, 55 (5): 36- 45. | |
Wang C Q , Jiao R Z , Dong Y H , et al. Differences in metabolic functions of soil microbial communities of Chinese fir plantations of different ages. Scientia Silvae Sinicae, 2019, 55 (5): 36- 45. | |
王立. 松嫩草地优势禾草生理生态的适应特性及其对模拟气候变化的响应. 长春: 东北师范大学硕士学位论文, 2006. | |
Wang L . Ecophysiology adaptation of dominant grasses and their response to simulated climatic changes in the Songnen grassland of China. Changchun: MS thesis of Northeast Normal University, 2006. | |
王苑. 气候变化背景下土壤微生物群落对干旱和自然环境大气CO2升高的响应. 上海: 东华大学硕士学位论文, 2014. | |
Wang Y . Response of soil microbial communities to drought and elevated CO2 under the background of climate change. Shanghai: MS thesis of Donghua University, 2014. | |
徐常青, 刘赛, 徐荣, 等. 我国枸杞主产区生产现状调研及建议. 中国中药杂志, 2014, 39 (11): 1979- 1984. | |
Xu C Q , Liu S , Xu R , et al. Investigation of production status in major wolfberry producing areas of China and some suggestions. China Journal of Chinese Materia Medica, 2014, 39 (11): 1979- 1984. | |
徐国强, 李杨, 史奕, 等. 开放式空气CO2浓度增高(FACE)对稻田土壤微生物的影响. 应用生态学报, 2002, 13 (10): 1358- 1359.
doi: 10.3321/j.issn:1001-9332.2002.10.034 |
|
Xu G Q , Li Y , Shi Y , et al. Effects of free-air CO2 enrichment on soil microbes in paddy field. Chinese Journal of Applied Ecology, 2002, 13 (10): 1358- 1359. | |
杨美玲, 张霞, 王绍明, 等. 基于高通量测序的裕民红花根际土壤细菌群落特征分析. 微生物学通报, 2018, 45 (11): 2429- 2438. | |
Yang M L , Zhang X , Wang S M , et al. High throughput sequencing analysis of bacterial communities in Yumin safflower. Microbiology China, 2018, 45 (11): 2429- 2438. | |
赵宗慈, 罗勇, 黄建斌. 回顾IPCC 30年(1988—2018年). 气候变化研究进展, 2018, 4 (5): 540- 546. | |
Zhao Z C , Luo Y , Huang J B . Review of IPCC for 30 years(1988—2018). Progress in Climate Change Research, 2018, 4 (5): 540- 546. | |
Carrillo Y , Dijkstra F , LeCain D , et al. Elevated CO2 and warming cause interactive effects on soil carbon and shifts in carbon use by bacteria. Ecology Letters, 2018, 21 (11): 1639- 1648.
doi: 10.1111/ele.13140 |
|
Delgado-Baquerizo M , Eldridge D J , Ochoa V , et al. Soil microbial communities drive the resistance of ecosystem multifunctionality to global change in drylands across the globe. Ecology Letters, 2017, 20 (10): 1295.
doi: 10.1111/ele.12826 |
|
Dijkstra F A , Morgan J A , Fischer J C , et al. Elevated CO2 and warming effects on CH4 uptake in a semiarid grassland below optimum soil moisture. Journal of Geophysical Research-Biogeosciences, 2011, 116 (G1): 79- 89. | |
Liu Y R , Delgado-Baquerizo M , Trivedi P , et al. Identity of biocrust species and microbial communities drive the response of soil multifunctionality to simulated global change. Soil Biology & Biochemistry, 2017, 107, 208- 217. | |
Ma Y P , Reddy V R , Devi M J , et al. De novo characterization of the Goji berry(Lycium barbarium L. ) fruit transcriptome and analysis of candidate genes involved in sugar metabolism under different CO2 concentrations. Tree Physiology, 2019, 39 (6): 1032- 1045. | |
McFarland J W , Waldrop M P , Haw M . Extreme CO2 disturbance and the resilience of soil microbial communities. Soil Biology & Biochemistry, 2013, 65 (5): 274- 286. | |
Mueller K E , LeCainD R , McCormack M L , et al. Root responses to elevated CO2, warming and irrigation in a semi-arid grassland: Integrating biomass, length and life span in a 5-year field experiment. Journal of Ecology, 2018, 106 (6): 2176- 2189.
doi: 10.1111/1365-2745.12993 |
|
Rier S T , Tuchman N C , Wetzel R G . Chemical changes to leaf litter from trees grown under elevated CO2 and the implications for microbial utilization in a stream ecosystem. Canadian Journal of Fisheries and Aquatic Sciences, 2005, 62 (1): 185- 194.
doi: 10.1139/f04-148 |
|
Rillig M C , Allen M F , Klironomos J N , et al. Plant species-specific changes in root-inhabiting fungi in a California annual grassland: responses to elevated CO2 and nutrients. Oecologia, 1998, 113 (2): 252- 259.
doi: 10.1007/s004420050376 |
|
Ronn R , Gavito M , Larsen J , et al. Response of free-living soil protozoa and microorganisms to elevated atmospheric CO2 and presence of mycorrhiza. Soil Biology & Biochemistry, 2002, 34 (7): 923- 932. | |
Terrer C , Vicca S , Stocker B D , et al. Ecosystem responses to elevated CO2 governed by plant-soil interactions and the cost of nitrogen acquisition. New Phytologist, 2018, 217 (2): 507- 522.
doi: 10.1111/nph.14872 |
|
Wang Y H , Yu Z H , Li Y S , et al. Elevated CO2 alters the structure of the bacterial community assimilating plant-derived carbon in the rhizosphere of soya bean. European Journal of Soil Science, 2019, 70 (6): 1212- 1220. | |
Williams M A , Rice C W , Owensby C E . Carbon dynamics and microbial activity in tallgrass prairie exposed to elevated CO2 for 8 years. Plant and Soil, 2000, 227 (1/2): 127- 137.
doi: 10.1023/A:1026590001307 |
|
Zak D R , Pregitzer K S , King J S , et al. Elevated atmospheric CO2, fine roots and the response of soil microorganisms: a review and hypothesis. New Phytologist, 2010, 147 (1): 201- 222. | |
Zhang T Y , Wu Y H , Zhuang L L , et al. Screening heterotrophic microalgal strains by using the Biolog method for biofuel production from organic wastewater. Algal Research-Biomass Biofuels and Bioproducts, 2014, 6 (5): 175- 179. |
[1] | Ha Rong, Ma Yaping, Cao Bing, Guo Fangyun, Song Lihua. Effects of Simulated Elevated CO2 Concentration on Vegetative Growth and Fruit Quality in Lycium barbarum [J]. Scientia Silvae Sinicae, 2019, 55(6): 28-36. |
[2] | Yang Ning, Zou Dongsheng, Yang Manyuan, Fu Meiyun, Lin Zhonggui, Zhao Linfeng. Variations of Soil Microbial Community Diversity in Purple Soils at Different Re-Vegetation Stages on Sloping-Land in Hengyang, Hunan Province [J]. Scientia Silvae Sinicae, 2016, 52(8): 146-156. |
[3] | Zhang Yuguang, Su Xiujiang, Cong Jing, Chen Zhan, Lu Hui, Liu Minchao, Li Diqiang. Variation of Soil Microbial Community along Elevation in the Shennongjia Mountain [J]. Scientia Silvae Sinicae, 2014, 50(9): 161-166. |
[4] | Wang Lin, Chen Zhan, Shang He. Effects of Ectomycorrhizal Fungi(Pisolithus tinctorius) of Masson Pine (Pinus massoniana)on Soil Microbial Metabolic Function under Simulated Acid Rain [J]. Scientia Silvae Sinicae, 2014, 50(7): 99-104. |
[5] | Zhang Xiaowen, Xing Shiyan, Wu Qikui, Liu Xiaojing. Effects of Nitrogen Addition on Chemical Composition of Soil Organic Carbon and Soil Microbial Community in a Young Ginkgo biloba Plantation [J]. Scientia Silvae Sinicae, 2014, 50(6): 115-124. |
[6] | Zhang QiongdaoJi BaozhongXu TianLiu ShuwenHuang SuhongWu GuangxiWang YanxianChen Zhengmei. Progress and Perspectives on the Genus Aphrodisium [J]. Scientia Silvae Sinicae, 2010, 46(11): 144-151. |
[7] | Luo Youqing;Zong Shixiang;Xu Zhichun;Zhang Jintong;Lu Changkuan;Zhang Liansheng. Integrated Management of Holcocerus hippophaecolus (Lepidoptera:Cossidae) [J]. Scientia Silvae Sinicae, 2007, 43(11): 146-150. |
[8] | Xu Huangcan;Yin Guangtian;Sun Qingpeng;Wu Jinkun. RESEARCH AND DEVELOPMENT OF RATTAN IN CHINA [J]. Scientia Silvae Sinicae, 2002, 38(2): 135-143. |
[9] | Zejun Tian,Yongbi Yan,Dingjiu Xia,Zhiguo Li. STUDIES ON THE MORPHOLOGY, BIO-ECOLOGICAL BEHAVIOUR AND ABUNDANT CHARACTERISTICS OF SPRING MIGRANT APHIS OF KABURAGIA OVATIRHUSTCOLA [J]. Scientia Silvae Sinicae, 1998, 34(1): 50-57. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||