秦岭太白山不同海拔锐齿栎林土壤微生物群落的变化特征

doi:10.11707/j.1001-7488.20211203

Abstract

Abstract:

Objective: This paper aims to study the dynamic changes and driving factors of soil microbial diversity and community composition in Quercus aliena var. acuteserrata forests at different altitudes in Qinling Mountains. Method: This experiment was conducted in Mount Taibai of Qinling Mountains with four elevations of 1 361.6, 1 524.2, 1 630.5 and 1 769.3 m, at which three standard plots (20 m ×20 m) were set in Q. aliena var. acuteserrata forests with good drainage, gentle slope and moderate growth. In August 2016, mixed sampling was carried out in 0-10 cm soil layers by the "S" type method, and then the diversity and composition of soil microbial community were determined by high-throughput sequencing technique. Result: 1) In Q. aliena var. acuteserrata forest, diversity (α diversity and β diversity) of soil bacteria had significant changes at different elevational gradients, but there was no significant change in diversity of soil fungi. 2) In the composition of bacterial community, the relative abundance of Actinobacteria decreased significantly with the increase of elevation, while that of Proteobacteria and Verrucomicrobia increased significantly. The relative abundance of Chloroflexi, Gemmatimonadetes and Nitrospirae showed a trend of increasing first and then decreasing; and that of other bacterial taxa such as Acidobacteria and Planctomycetes showed a decreasing trend along the increase of elevation, but there was no significant difference between different elevations. In the composition of fungal community, the relative abundance of Basidiomycota and Ascomycota, two dominant phyla, did not change significantly with the increase of elevation. 3) Bacterial diversity was significantly positively correlated with soil organic carbon (SOC), total nitrogen (TN), C∶P and N∶P, and the response of bacterial community composition to elevational gradient was mainly determined by soil bulk density, soil temperature, SOC, C∶P and TN. Fungal diversity was only significantly negatively correlated with soil moisture, and its community composition was significantly correlated with pH, SOC, TN and N∶P. Conclusion: This study indicates that the changes in soil bacterial and fungal diversity along the altitudinal gradient are influenced by different soil environmental factors, and provides a comprehensive insight into the changing characteristics and influencing factors of soil microbial community structure and composition in the warm temperate forest ecosystems of the Qinling Mountains, thus providing a scientific basis for an in-depth understanding of the microbial mechanisms of soil organic carbon decomposition in forest ecosystems.

Key words: forest ecosystem, elevational gradients, Quercus aliena var. acuteserrata forest, Illumina sequencing, soil microbial community, soil nutrients

CLC Number:

Q931
S714.3

Yi Li,Xiuxiu Feng,Fazhu Zhao,Yaoxin Guo,Jun Wang,Chengjie Ren. Structure Characteristics of Soil Microbial Community in Quercus aliena var. acuteserrata Forests at Different Altitudes in Qinling Mountains[J]. Scientia Silvae Sinicae, 2021, 57(12): 22-31.

Figures/Tables 7

Table 1

Fig.1

Fig.2

Fig.3

Fig.4

Table 2

Fig.5

References 0

	贺婧, 闫冰, 李俊生, 等. 秦岭中段北坡不同海拔土壤中细菌群落的分布特征及区域差异比较. 环境科学研究, 2019, 32 (8): 1374- 1383.
	He Q , Yan B , Li J S , et al. Altitude distribution patterns and regional differences of soil bacterial community in northern slopes in the middle Qinling Mountains. Research of Environmental Sciences, 2019, 32 (8): 1374- 1383.
	雷梅, 陈同斌, 冯立孝, 等. 太白山北坡成土因素及不同土壤垂直带谱的比较. 地理研究, 2001, 20 (5): 583- 592. doi: 10.3321/j.issn:1000-0585.2001.05.008
	Lei M , Chen T B , Feng L X , et al. Soil formation factors and comparison among different altitudinal zonations of the soils on northern slope of the Taibai mountain. Geographical Research, 2001, 20 (5): 583- 592. doi: 10.3321/j.issn:1000-0585.2001.05.008
	李超男, 李家宝, 李香真. 贡嘎山海拔梯度上不同植被类型土壤甲烷氧化菌群落结构及多样性. 应用生态学报, 2017, 28 (3): 805- 814.
	Li C N , Li J B , Li X Z . Soil methanotrophic community structure and diversity in different vegetation types at elevation gradient of Gongga Mountain, Southwest China. Chinese Journal of Applied Ecology, 2017, 28 (3): 805- 814.
	李桂香, 马克明. 土壤微生物多样性海拔格局研究进展. 生态学报, 2018, 38 (5): 1521- 1529.
	Li G X , Ma K M . Progress in the study of elevational patterns of soil microbial diversity. Acta Ecologica Sinica, 2018, 38 (5): 1521- 1529.
	马新萍, 白红英, 郭帅, 等. 秦岭太白山气温垂直递减率研究. 干旱区资源与环境, 2017, 31 (7): 139- 144.
	Ma X P , Bai H Y , Guo S , et al. Verification of temperature vertical lapse rate and mountain climate characteristics of Taibai Mountains in Qinling Mountains. Journal of Arid Land Resources and Environment, 2017, 31 (7): 139- 144.
	牛晓栋, 刘晓静, 刘世荣, 等. 亚热带-暖温带过渡区天然栎林的能量平衡特征. 生态学报, 2018, 38 (18): 6701- 6711.
	Niu X D , Liu X J , Liu S R , et al. Energy balance characteristics of a natural oak forest(Quercus aliena) at a transitional area from a subtropical to warmtemperate climate, China. Acta Ecologica Sinica, 2018, 38 (18): 6701- 6711.
	秦进, 白红英, 刘荣娟, 等. 近144年来秦岭太白山林线区3—6月平均气温的重建. 生态学报, 2017, 37 (22): 7585- 7594.
	Qin J , Bai H Y , Liu R J , et al. Reconstruction of March-June mean air temperature along the timberline of Mount Taibai, Qinling mountains, northwest China, over the last 144 years. Acta Ecologica Sinica, 2017, 37 (22): 7585- 7594.
	王淼, 曲来叶, 马克明, 等. 罕山土壤微生物群落组成对植被类型的响应. 生态学报, 2014, 34 (22): 6640- 6654.
	Wang M , Qu L Y , Ma K M , et al. Response of soil microbial community composition to vegetation types. Acta Ecologica Sinica, 2014, 34 (22): 6640- 6654.
	张善红, 白红英, 高翔, 等. 太白山植被指数时空变化及其对区域温度的响应. 自然资源学报, 2011, 26 (8): 1377- 1386.
	Zhang S H , Bai H Y , Gao X , et al. Spatial-temporal changes of vegetation index and its responses to regional temperature in Taibai Mountain. Journal of Natural Resources, 2011, 26 (8): 1377- 1386.
	赵路红, 李昌珍, 康迪, 等. 黄土丘陵区植被恢复对土壤可溶性氮组分的影响. 生态学报, 2017, 37 (10): 3533- 3542.
	Zhao L H , Li C Z , Kang D , et al. Effects of vegetation restoration on soil soluble nitrogen in the Loess Hilly Region. Acta Ecologica Sinica, 2017, 37 (10): 3533- 3542.
	赵盼盼, 周嘉聪, 林开淼, 等. 海拔梯度变化对中亚热带黄山松土壤微生物生物量和群落结构的影响. 生态学报, 2019, 39 (6): 2215- 2225.
	Zhao P P , Zhou J C , Lin K M , et al. Effect of different altitudes on soil microbial biomass and community structure of Pinus taiwanensis forest in mid-subtropical zone. Acta Ecologica Sinica, 2019, 39 (6): 2215- 2225.
	周世兴, 邹秤, 肖永翔, 等. 模拟氮沉降对华西雨屏区天然常绿阔叶林土壤微生物生物量碳和氮的影响. 应用生态学报, 2017, 28 (1): 12- 18.
	Zhou S X , Zou C , Xiao Y X , et al. Effects of simulated nitrogen deposition on soil microbial biomass carbon and nitrogen in natural evergreen broad-leaved forest in the Rainy Area of West China. Chinese Journal of Applied Ecology, 2017, 28 (1): 12- 18.
	Bach L H , Grytnes J A , Halvorsen R , et al. Tree influence on soil microbial community structure. Soil Biology and Biochemistry, 2010, 42 (11): 1934- 1943. doi: 10.1016/j.soilbio.2010.07.002
	Bahram M , Polme S , Koljalg U , et al. Regional and local patterns of ectomycorrhizal fungal diversity and community structure along an altitudinal gradient in the Hyrcanian forests of northern Iran. New Phytologist, 2012, 193 (2): 465- 473. doi: 10.1111/j.1469-8137.2011.03927.x
	Caporaso J G , Lauber C L , Walters W A , et al. Ultra-high-throughput microbial community analysis on the Illumina Hiseq and MiSeq platforms. Isme Journal, 2012, 6 (8): 1621- 1624. doi: 10.1038/ismej.2012.8
	Kielak A , Pijl A S , van Veen J A , et al. Phylogenetic diversity of Acidobacteria in a former agricultural soil. Isme Journal, 2009, 3 (3): 378- 382. doi: 10.1038/ismej.2008.113
	Kulhánková A , Béguiristain T , Moukoumi J , et al. Spatial and temporal diversity of wood decomposer communities in different forest stands, determined by its rdna targeted tgge. Annals of Forest Science, 2006, 63 (5): 547- 556. doi: 10.1051/forest:2006037
	Lucas Y , Luizao F J , Chauvel A . The relation between biological activity of the rain forest and mineral composition of soils. Science, 1993, 260 (5170): 521- 523.
	Meng H , Li K , Nie M , et al. Responses of bacterial and fungal communities to an elevation gradient in a subtropical montane forest of China. Applied Microbiology and Biotechnology, 2013, 97 (5): 2219- 2230. doi: 10.1007/s00253-012-4063-7
	Michelsen A , Graglia E , Schmidt I K , et al. Differential responses of grass and a dwarf shrub to long-term changes in soil microbial biomass C, N and P following factorial addition of N P K fertilizer, fungicide and labile carbon to a heath. New Phytologist, 1999, 143 (3): 523- 538. doi: 10.1046/j.1469-8137.1999.00479.x
	Moreira Neto S L , Matheus D R , Gomes Machado K M . Influence of pH on the growth, laccase activity and rbbr decolorization by tropical Basidiomycetes. Brazilian Archives of Biology and Technology, 2009, 52 (5): 1075- 1082. doi: 10.1590/S1516-89132009000500003
	Naether A , Foesel B U , Naegele V , et al. Environmental factors affect Acidobacterial communities below the subgroup level in grassland and forest soils. Applied and Environmental Microbiology, 2012, 78 (20): 7398- 7406. doi: 10.1128/AEM.01325-12
	Ren C , Zhang W , Zhong Z , et al. Differential responses of soil microbial biomass, diversity, and compositions to altitudinal gradients depend on plant and soil characteristics. Science of the Total Environment, 2018, 610, 750- 758.
	Ruiz-Gonzalez C , Salazar G , Logares R , et al. Weak coherence in abundance patterns between bacterial classes and their constituent OTUs along a regulated river. Frontiers in Microbiology, 2015, 6, 1293.
	Shen C , Liang W , Shi Y , et al. Contrasting elevational diversity patterns between eukaryotic soil microbes and plants. Ecology, 2014, 95 (11): 3190- 3202. doi: 10.1890/14-0310.1
	Shen C , Ni Y , Liang W , et al. Distinct soil bacterial communities along a small-scale elevational gradient in alpine tundra. Frontiers in Microbiology, 2015, 6, 1293.
	Shen C , Xiong J , Zhang H , et al. Soil pH drives the spatial distribution of bacterial communities along elevation on Changbai Mountain. Soil Biology & Biochemistry, 2013, 57, 204- 211.
	Siles J A , Margesin R . Abundance and diversity of bacterial, archaeal, and fungal communities along an altitudinal gradient in Alpine forest soils: What are the driving factors?. Microbial Ecology, 2016, 72 (1): 207- 220. doi: 10.1007/s00248-016-0748-2
	Stroobants A , Degrune F , Olivier C , et al. Diversity of bacterial communities in a profile of a winter wheat field: known and unknown members. Microbial Ecology, 2014, 68 (4): 822- 833. doi: 10.1007/s00248-014-0458-6
	van der Heijden M G A , Bardgett R D , van Straalen N M . The unseen majority: soil microbes as drivers of plant diversity and productivity in terrestrial ecosystems. Ecology Letters, 2008, 11 (3): 296- 310. doi: 10.1111/j.1461-0248.2007.01139.x
	Walters W , Hyde E R , Berg-Lyons D , et al. Improved bacterial 16S rRNA gene(V4 and V4-5) and fungal internal transcribed spacer marker gene primers for microbial community surveys. Msystems, 2016, 1 (1): 1- 10.
	Wang J T , Zheng Y M , Hu H W , et al. Soil pH determines the alpha diversity but not beta diversity of soil fungal community along altitude in a typical Tibetan forest ecosystem. Journal of Soils and Sediments, 2015, 15 (5): 1224- 1232. doi: 10.1007/s11368-015-1070-1
	Wang N F , Zhang T , Yang X , et al. Diversity and composition of bacterial community in soils and lake sediments from an Arctic lake area. Frontiers in Microbiology, 2016, 7, 2140.
	Xu Z , Yu G , Zhang X , et al. The variations in soil microbial communities, enzyme activities and their relationships with soil organic matter decomposition along the northern slope of Changbai Mountain. Applied Soil Ecology, 2015, 86, 19- 29. doi: 10.1016/j.apsoil.2014.09.015
	Zhang Y , Cong J , Lu H , et al. Community structure and elevational diversity patterns of soil Acidobacteria. Journal of Environmental Sciences, 2014, 26 (8): 1717- 1724. doi: 10.1016/j.jes.2014.06.012

指标 Index	海拔Elevation/m				F_{(3, 8)}	P
指标 Index	1 361.6	1 524.2	1 630.5	1 769.3	F_{(3, 8)}	P
pH	6.06±0.04A	6.03±0.02AB	6.06±0.01A	5.98±0.05B	4.08	0.05^*
BD/(g·cm^-3)	1.45±0.04A	1.32±0.03B	1.18±0.03C	1.02±0.02D	99.44	＜0.01^**
ST/℃	16.07±0.32A	16.02±0.38A	15.87±0.28A	15.01±0.48B	5.29	0.03^*
SM(%)	25.97±1.46A	27.59±2.67A	26.69±1.11A	27.49±1.27A	0.58	0.65
SOC/(g·kg^-1)	19.72±1.88C	31.32±3.19A	23.87±0.72B	25.80±1.95B	15.41	＜0.01^**
TN/(g·kg^-1)	1.09±0.33B	2.24±0.45A	1.51±0.38AB	1.66±0.29AB	5.01	0.03^*
TP/(g·kg^-1)	0.35±0.02A	0.40±0.02A	0.39±0.04A	0.39±0.03A	1.75	0.23
C∶N	18.92±3.99A	14.42±3.51A	16.54±4.41A	15.92±3.54A	0.70	0.58
C∶P	56.24±3.54B	78.80±10.66A	61.55±6.29B	65.55±4.47B	6.00	0.02^*
N∶P	3.08±0.78B	5.65±1.24A	3.83±0.64B	4.20±0.59AB	4.79	0.03^*
NH₄⁺/(mg·kg^-1)	68.63±5.64B	68.17±2.27B	80.60±3.12A	65.67±5.31B	7.15	0.01^**
NO₃^-/(mg·kg^-1)	4.54±0.80B	5.34±1.04B	8.14±2.13A	6.23±2.34AB	2.45	0.14
MBC/(mg·kg^-1)	452.86±10.14A	478.12±32.36A	464.99±40.04A	506.76±38.18A	1.53	0.28
MBN/(mg·kg^-1)	111.48±7.10A	135.80±9.14A	119.48±14.71A	115.01±31.05A	1.06	0.42

土壤性质 Soil property	细菌多样性 Bacterial diversity		真菌多样性 Fungal diversity
土壤性质 Soil property	香农指数 Shannon index	Bray-Curtis distance	香农指数 Shannon index	Bray-Curtis distance
SOC	0.699^*	0.778^**	0.084	0.53
TN	0.420	0.755^**	-0.077	0.43
TP	0.221	0.576^*	0.091	0.029
C∶N	0.000	-0.175	0.077	0.282
C∶P	0.797^**	0.648^*	0.091	0.617
N∶P	0.343	0.717^**	-0.175	0.474
NH₄⁺	0.091	0.049	-0.462	-0.11
NO₃^-	0.203	0.305	0.559	-0.086
pH	0.137	-0.318	-0.274	-0.171
BD	-0.189	-0.669^*	-0.224	-0.445
ST	0.007	-0.251	0.070	-0.151
SM	0.161	0.32	-0.594^*	0.376

[1]	Yanlin Zhang,Caifeng Huang,Mingzhuo Bao,Chuifan Zhou,Zongming He. Effects of Biochar and Its Aging Biochar on Soils Nutrients and Microbial Community Composition in Cunninghamia lanceolata Plantations: a Laboratory Simulation Experiment [J]. Scientia Silvae Sinicae, 2021, 57(6): 169-179.
[2]	Yun Xie,Fangyun Guo,Lihua Chen,Bing Cao. Effects of Elevated CO₂ Concentration on Soil Microbial Functional Diversity and Carbon Source Utilization Characteristics in the Root Zone of Lycium barbarum [J]. Scientia Silvae Sinicae, 2021, 57(4): 163-172.
[3]	Haiqing Hu,Bizhen Luo,Sisheng Luo,Shujing Wei,Zhenshi Wang,Xiaochuan Li,Fei Liu. Research Progress on Effects of Forest Fire Disturbance on Carbon Pool of Forest Ecosystem [J]. Scientia Silvae Sinicae, 2020, 56(4): 160-169.
[4]	Chen Wei, Yang Fei, Wang Juanle, Cheng Shulan. Integrated Quantitative Evaluation of Resilience of Subtropical Forest Ecosystem Disturbed by Freezing Ice and Snow Frozen Disaster: Take Daoxian County for Example [J]. Scientia Silvae Sinicae, 2018, 54(6): 1-8.
[5]	Yang Ning, Zou Dongsheng, Yang Manyuan, Fu Meiyun, Lin Zhonggui, Zhao Linfeng. Variations of Soil Microbial Community Diversity in Purple Soils at Different Re-Vegetation Stages on Sloping-Land in Hengyang, Hunan Province [J]. Scientia Silvae Sinicae, 2016, 52(8): 146-156.
[6]	Zhang Yuguang, Su Xiujiang, Cong Jing, Chen Zhan, Lu Hui, Liu Minchao, Li Diqiang. Variation of Soil Microbial Community along Elevation in the Shennongjia Mountain [J]. Scientia Silvae Sinicae, 2014, 50(9): 161-166.
[7]	Wang Lin, Chen Zhan, Shang He. Effects of Ectomycorrhizal Fungi(Pisolithus tinctorius) of Masson Pine (Pinus massoniana)on Soil Microbial Metabolic Function under Simulated Acid Rain [J]. Scientia Silvae Sinicae, 2014, 50(7): 99-104.
[8]	Zhang Xiaowen, Xing Shiyan, Wu Qikui, Liu Xiaojing. Effects of Nitrogen Addition on Chemical Composition of Soil Organic Carbon and Soil Microbial Community in a Young Ginkgo biloba Plantation [J]. Scientia Silvae Sinicae, 2014, 50(6): 115-124.
[9]	Dong Lili, Chen Yi, Li Xiaohua. Effects of the Returning Farmland to Forests on Content of Water Stable Soil Aggregates and the Nutrients in Wuqi County [J]. Scientia Silvae Sinicae, 2014, 50(5): 140-146.
[10]	Xiang Zeyu, Zhang Li, Zhang Quanfa, Liu Wei, Wang Genxu, Wang Changting, Hu Lei. Soil Nutrients and Microbial Functional Diversity of Different Stand Types in Qinghai Province [J]. Scientia Silvae Sinicae, 2014, 50(4): 22-31.
[11]	Yang Ning, Zou Dongsheng, Yang Manyuan, Fu Meiyun, Lei Yulan, Lin Zhonggui, Zhao Linfeng. Dynamic Changes of Soil Microbial Biomass and Soil Nutrients along Re-Vegetation on Sloping-Land with Purple Soils in Hengyang of Hunan Province, South-Central China [J]. Scientia Silvae Sinicae, 2014, 50(12): 144-150.
[12]	Deng Minjie, Dong Yanpeng, Zhao Zhenli, Zhang Xiaoshen, Fan Guoqiang. Illumina-Based de novo Sequencing and Characterization of the Transcriptome of Paulownia Plant [J]. Scientia Silvae Sinicae, 2013, 49(6): 30-36.
[13]	Tao Yuzhu, Di Xueying. Fire Interference on Forest Soil Microbial Communities and the Mechanism：A Review [J]. Scientia Silvae Sinicae, 2013, 49(11): 146-157.
[14]	Guo Jianfen;Yang Yusheng;Zhong Xianfang;He Xudong. Storage, Carbon Pool of Coarse Woody Debris in Forest Ecosystems and the Influence Factors [J]. Scientia Silvae Sinicae, 2011, 47(2): 125-133.
[15]	Wang Bing;Ren Xiaoxu;Hu Wen. Assessment of Forest Ecosystem Services Value in China [J]. Scientia Silvae Sinicae, 2011, 47(2): 145-153.

Structure Characteristics of Soil Microbial Community in Quercus aliena var. acuteserrata Forests at Different Altitudes in Qinling Mountains

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 7

References 0

Related Articles 15

Recommended Articles

Metrics

Comments