光合色素含量差异对花叶唐竹不同叶色表型光合特性的影响

doi:10.11707/j.1001-7488.20191203

摘要/Abstract

摘要：

目的: 研究光合色素含量差异对花叶唐竹4种叶色表型光合特性的影响，为花叶竹类的栽培和选育提供参考。方法: 以花叶唐竹2年生分株苗为研究对象，测定全绿、绿底白纹、白底绿纹、全白4种表型叶片的光合色素含量、光合生理参数及叶绿素荧光参数，分别观察绿色叶肉细胞和白色叶肉细胞的超微结构。结果: 4种表型间叶片光合色素含量差异显著，且随着叶片绿色面积比例的下降而呈现显著下降趋势；花叶唐竹绿色叶肉细胞的细胞器排列紧凑且十分完整，叶绿体发育健全，膜系统完善；在大多数白色叶肉细胞中，没有发现完整的叶绿体，而是发育不良的叶绿体和白色体；4种表型叶片的叶绿素含量与其净光合速率、气孔导度、蒸腾速率及水分利用率呈正相关，与胞间二氧化碳浓度呈负相关；全白叶片由于缺乏PSⅡ光反应中心，原初光能转化能力低下，且无法进行光抑制的一系列自我保护反应。结论: 花叶唐竹白色叶肉细胞中叶绿体发育缺陷导致其叶绿素合成受阻，进而影响光合能力，使其净光合速率全为负值，且其自我保护机制较差，强光下易被灼伤，故需要予以适当遮荫。

关键词: 花叶唐竹, 光合色素, 叶绿体结构, 光合特性, 叶绿素荧光

Abstract:

Objectve: Sinobambusa tootsik f. luteoloalbostriata is a bamboo species with creeping rhizomes, various levels of leave variegation and small- or medium-diameter stems, and has high ornamental value. This study aims to investigate the influence in photosynthetic capacity caused by variation in photosynthetic pigment content of four phenotypes of S.tootsik f. luteoloalbostriata cultivation, to lay a certain foundation for the cultivation and breeding of the bamboos with leave variegation. Method: Two-year-old seedlings of S. tootsik f. luteoloalbostriata were taken as materials. Photosynthetic pigment content, photosynthetic parameters and chlorophyll fluorescence parameters of the leaves of four phenotypes, including the whole green leaves, green leaves with white stripes, white leaves with green stripes and whole white leaves, were measured, and ultrastructure of green and white mesophyll cells were observed as well. Result: There was a significant difference in photosynthetic pigment content among four phenotypes, and it showed a significant reduction trend as the green area decreased in the leaves. The organelles of green mesophyll cells in S. tootsik f. luteoloalbostriata mesophyll were compact and intact, the chloroplast was well developed, and the membrane system was also well developed. In most white mesophyll cells, no complete chloroplast was found. Instead, degraded chloroplasts and etioplasts were found. The chlorophyll content was positively correlated with net photosynthetic rate, stomatal conductance, transpiration rate and water use efficiency, but negatively correlated with the intercellular CO₂ concentration. The original light energy capacity was low in white leaves due to the lack of PSⅡ reaction center, and a series self-protection of light inhibition could not be carried out. Conclusion: The developmental defect of chloroplasts in white mesophyll cells of S. tootsik f. luteoloalbostriata obstructed the chlorophyll synthesis, which affected the photosynthetic ability, so that the net photosynthetic rate was negative. With a poor self-protection mechanism, white leaves are easy to be burned under strong light, so appropriate shade is needed.

Key words: Sinobambusa tootsik f. luteoloalbostriata, photosynthetic pigment, chloroplast structure, photosynthetic characteristics, chlorophyll fluorescence

中图分类号:

S718.51

陈凌艳,谢德金,荣俊冬,赖金莉,林雪玲,郑郁善. 光合色素含量差异对花叶唐竹不同叶色表型光合特性的影响[J]. 林业科学, 2019, 55(12): 21-31.

Lingyan Chen,Dejin Xie,Jundong Rong,Jinli Lai,Xueling Lin,Yushan Zheng. Effects of Photosynthetic Pigment Content on Photosynthetic Characteristics of Different Leaf Color Phenotypes of Sinobambusa tootsik f. luteoloalbostriata[J]. Scientia Silvae Sinicae, 2019, 55(12): 21-31.

图/表 7

表1

图1

图2

表2

图3

表3

表4

参考文献 0

	陈凌艳, 何丽婷, 赖金莉, 等. 银丝竹不同叶色叶绿素合成及叶结构差异. 森林与环境学报, 2017. 37 (4): 385- 391.
	Chen L Y , He L T , Lai J L , et al. The variation of chlorophyll biosynthesis and the structure in different color leaves of Bambusa multiplex 'Silverstripe'. Journal of Forest and Environment, 2017. 37 (4): 385- 391.
	陈松河, 王振忠. 唐竹属一新变种——花叶唐竹. 竹子研究汇刊, 2005. 24 (4): 12. doi: 10.3969/j.issn.1000-6567.2005.04.003
	Chen S H , Wang Z Z . Sinobambusa tootsik Makino ex Nakai var. luteolo-albo-striata S. H. Chen ex Z. Z. Wang, a new variety of Sinobambusa from Xiamen, China. Journal of Bamboo Research, 2005. 24 (4): 12. doi: 10.3969/j.issn.1000-6567.2005.04.003
	刘梦洋, 卢银, 赵建军, 等. 大白菜叶色突变体的HRM鉴定及叶绿素荧光参数分析. 园艺学报, 2014. 41 (11): 2215- 2224.
	Liu M Y , Lu Y , Zhao J J , et al. HRM identification and chlorophyll fluorescence characteristics on leaf color mutants in Chinese cabbage. Acta Horticulturae Sinica, 2014. 41 (11): 2215- 2224.
	吕明, 刘海衡, 毛虎德, 等. 芥菜型油菜黄化突变体叶片叶绿素合成代谢变化. 西北植物学报, 2010. 30 (11): 2177- 2183.
	Lü M , Liu H H , Mao H D , et al. Changes of chlorophyll biosynthesis metabolism in chlorophyll-deficient mutant in Brassica juncea.. Acta Boreali-Occidentalia Sinica, 2010. 30 (11): 2177- 2183.
	宋旭丽, 胡春梅, 孟静静, 等. NaCl胁迫加重强光胁迫下超大甜椒叶片的光系统Ⅱ和光系统Ⅰ的光抑制. 植物生态学报, 2011. 35 (6): 681- 686.
	Song X L , Hu C M , Meng J J , et al. NaCl stress aggravates photoinhibition of photosystem Ⅱ and photosystem I in Capsicum annuum leaves under high irradiance stress. Chinese Journal of Plant Ecology, 2011. 35 (6): 681- 686.
	王啸晨, 岳祥华, 吴杰, 等. 2种观赏彩叶竹形态结构的观察与分析. 中国农学通报, 2012. 28 (16): 233- 238.
	Wang X C , Yue X H , Wu J , et al. Appearance and structure analysis of chimeric leaves from two ornamental bamboos. Chinese Agricultural Science Bulletin, 2012. 28 (16): 233- 238.
	吴志庄, 高贵宾, 欧建德, 等. 生物炭肥对毛竹林下三叶青叶绿素含量、光合与荧光特性的影响. 西北林学院学报, 2017. 32 (5): 59- 63, 103. doi: 10.3969/j.issn.1001-7461.2017.05.11
	Wu Z Z , Gao G B , Ou J D , et al. Effects of the application of biochar-based fertilizer on chlorophyll contents and photosynthesis & fluorescence characteristics of Tetrastigma hemsleyanum under Moso bamboo forest. Journal of Northwest Forestry University, 2017. 32 (5): 59- 63, 103. doi: 10.3969/j.issn.1001-7461.2017.05.11
	徐坤, 邹琦, 郑国生. 强光下姜叶片的光呼吸及叶黄素循环. 园艺学报, 2002. 29 (1): 47- 51. doi: 10.3321/j.issn:0513-353X.2002.01.011
	Xu K , Zou Q , Zheng G S . Photorespiration and xanthophyll cycle in adaptation to strong light in ginger leaves. Acta Horticulturae Sinica, 2002. 29 (1): 47- 51. doi: 10.3321/j.issn:0513-353X.2002.01.011
	杨富军, 赵长星, 闫萌萌, 等. 栽培方式对夏直播花生叶片光合特性及产量的影响. 应用生态学报, 2013. 24 (3): 747- 752.
	Yang F J , Zhao C X , Yan M M , et al. Effects of different cultivation modes on the leaf photosynthetic characteristics and yield of summer-sowing peanut. Chinese Journal of Applied Ecology, 2013. 24 (3): 747- 752.
	杨海芸. 2015.花叶矢竹叶色变异机理研究.北京:北京林业大学博士学位论文.
	Yang H Y. 2015. Study on mechanism of leaf color variation of Pseudosasa japonica f. akebonosuji. Beijing: PhD thesis of Beijing Forestry University.[in Chinese]
	叶子飘, 张海利, 黄宗安, 等. 叶片光能利用效率和水分利用效率对光响应的模型构建. 植物生理学报, 2017. 53 (6): 1116- 1122.
	Ye Z P , Zhang H L , Huang Z A , et al. Model construction of light use efficiency and water use efficiency based on a photosynthetic mechanistic model of light response. Plant Physiology Communication, 2017. 53 (6): 1116- 1122.
	叶子飘, 杨小龙, 康华靖, 等. C3和C4植物光能利用效率和水分利用效率的比较研究. 浙江农业学报, 2016. 28 (11): 1867- 1873. doi: 10.3969/j.issn.1004-1524.2016.11.10
	Ye Z P , Yang X L , Kang H J , et al. Comparison of light-use and water-use efficiency for C3 and C4 species. Acta Agriculturae Zhejiangensis, 2016. 28 (11): 1867- 1873. doi: 10.3969/j.issn.1004-1524.2016.11.10
	张帆, 万雪琴, 翟晶. 镉处理下增施氮对杨树叶绿素合成和叶绿体超微结构的影响. 核农学报, 2014. 28 (3): 485- 491.
	Zhang F , Wan X Q , Zhai J . Effects of nitrogen supplement on chlorophyll synthesis and chloroplast ultrastructure of poplar plants under cadmium stress. Journal of Nuclear Agricultural Sciences, 2014. 28 (3): 485- 491.
	郑国生, 何秀丽. 夏季遮荫改善大田牡丹叶片光合功能的研究. 林业科学, 2006. 42 (4): 27- 32.
	Zheng G S , He X L . Studies on the photosynthetic improvement in the leaves of field tree peony through shading treatment in summer. Scientia Silvae sinicae, 2006. 42 (4): 27- 32.
	Allen J F . A redox switch hypothesis for the origin of two light reactions in photosynthesis. FFBS Letters, 2005. 579 (5): 963- 968. doi: 10.1016/j.febslet.2005.01.015
	Apel K , Gollmer I , Batschauer A . The light-depend control of chloroplast development in barley (Hordeum vulgare L.). Journal of Cellular Biochemistry, 1983. 23 (1-4): 181- 189. doi: 10.1002/jcb.240230115
	Bertamini M , Nedunchezhian N . Photoinhibition of photobiosynthesis in mature and young leaves of grapevine (Vitis vinifera L.). Plant Science, 2003. 164 (4): 635- 644. doi: 10.1016/S0168-9452(03)00018-9
	Björkman O , Demmig B . Photon yield of O₂ evolution and chlorophyll fluorescence characteristics at 77K among vascular plants of diverse origins. Planta, 1987. 170 (4): 489- 504. doi: 10.1007/BF00402983
	Chen L Y , Lai J L , He T Y , et al. Differences in photosynthesis of variegated temple bamboo leaves with various levels of variegation are related to chlorophyll biosynthesis and chloroplast development. Journal of American Society for Horticulture Science, 2018. 143 (2): 144- 153. doi: 10.21273/JASHS04359-18
	ChenY S , Chesson P , Wu H W , et al. Leaf structure affects a plant's appearance:combined multiple-mechanisms intensify remarkable foliar variegation. Journal of Plant Research, 2017. 130 (2): 311- 325. doi: 10.1007/s10265-016-0890-4
	Chen W , Yang X , He Z , et al. Differential changes in photosynthetic capacity, 77K chlorophyll fluorescence and chloroplast ultrastructure between Zn-efficient and Zn-inefficient rice genotypes (Oryza sativa) under low zinc stress. Physiologia Plantarum, 2008. 132 (1): 89- 101.
	Demmig B , Björkman O . Comparison of the effect of excessive light on chlorophyll fluorescence(77k) photon yield of O₂ evolution in leaves of higher plants. Planta, 1987. 171 (2): 171- 184. doi: 10.1007/BF00391092
	Genty B , Harbinson J , Briantais J M , et al. The relationship between non-photochemical quenching of chlorophyll fluorescence and the rate of photosystem 2 photochemistry in leaves. Photosynthesis Research, 1990. 25 (3): 249- 257. doi: 10.1007/BF00033166
	Jackowski G , Kacprzak K Jansson S . Identification of Lhcb1/Lhcb2/Lhcb3 heterotrimers of the main light-harvesting chlorophyll a/b-protein complex of photosystem Ⅱ(LHCⅡ). Biochimica et-Biophysica-Acta, 2001. 1504 (2/3): 340- 345.
	Jiang K Y , Zhou M B , Yang H Y , et al. Cloning and functional characterization of PjCAO gene involved in chlorophyll b biosynthesis in Pseudosasa japonica cv.Akebonosuji.. Trees, 2016. 30 (4): 1303- 1314. doi: 10.1007/s00468-016-1367-8
	Lichtenthaler H K , Wellburn A R . Determination of total caroteinods and chlorophylls a and b of leaf in different solvents. Biochemical Society Transactions, 1983. 11 (5): 591- 592. doi: 10.1042/bst0110591
	Lu C , Lu Q , Zhang J , et al. Characterization of photosynthetic pigment composition, photosystem Ⅱ photochemistry and thermal energy dissipation during leaf senescence of wheat plants grown in the field. Journal of Experimental Botany, 2001. 52 (362): 1805- 1810. doi: 10.1093/jexbot/52.362.1805
	Lu C , Zhang J . Modifications in photosystem Ⅱ photochemistry in senescent leaves of maize plants. Journal of Experimental Botany, 1998. 49 (327): 1671- 1679. doi: 10.1093/jxb/49.327.1671
	Manohara M S , Tripathy B C . Regulation of protoporphyrin Ⅸ biosynthesis by intraplastidic compartmentalization and adenosine triphosphate. Planta, 2000. 212 (1): 52- 59. doi: 10.1007/s004250000363
	Müller P , Li X P , Niyogi K K . Non-photochemical quenching. A response to excess light energy. Plant Physiology, 2001. 125 (4): 1558- 1566.
	Nakanishi H , Nozue H , Suzuki K , et al. Characterization of the Arabidopsis thaliana Mutant pcb2 which accumulates divinyl chlorophylls. Plant & Cell Physiology, 2005. 46 (3): 467- 473.
	Nathan N , Adam B S H . The complex architecture of oxygenic photosynthesis. Nature Review Molecular Cell Biology, 2004. 5 (12): 971- 982. doi: 10.1038/nrm1525
	Ögren E . Prediction of photoinhibition of photosynthesis from measurements of fluorescence quenching components. Planta, 1991. 184 (4): 538- 544.
	Perveen S , Shahbaz M , Ashraf M . Regulation in gas exchangeand quantum yield of photosystem Ⅱ (PSⅡ) in salt-stressed and non-stressed wheat plants raised from seed treated with triacontanol. Pakistan Journal of Botany, 2010. 42 (5): 3073- 3081.
	Pinheiro H A , Silva J V , Endres L . Leaf gas exchange, chloroplastic pigments and dry matter accumulation in castor bean (Ricinus communis L.) seedlings subjected to salt stress conditions. Industrial Crops and Products, 2008. 27 (3): 385- 392. doi: 10.1016/j.indcrop.2007.10.003
	Sheue C , Pao S , Chien L , et al. Natural foliar variegation without costs? The case of Begonia.. Annals of Botany, 2012. 109 (6): 1065- 1074. doi: 10.1093/aob/mcs025
	Singh S K , Reddy V R , Fleisher D H , et al. Relationship between photosynthetic pigments and chlorophyll fluorescence in soybean under varying phosphorus nutrition at ambient and elevated CO₂. Photosynthetica, 2016. 55 (3): 1- 13.
	Slattery R A , VanLooke A , Bernacchi C J , et al. Photosynthesis, light use efficiency, and yield of reduced-chlorophyll soybean mutants in field conditions. Frontiers in Plant Science, 2017. 8, 549.
	Snider J L , Chastain D R , Meeks C D , et al. Predawn respiration rates during flowering are highly predictive of yield response in Gossypium hirsutum when yield variability is water-induced. Journal of Plant Physiology, 2015. 183 (2015): 114- 120.
	Soldatini G F , Nali C , Guidi L , et al. Photosynthesis of Hedera canariensis var. azorica variegated leaves as affected by ozone. Photosynthetica, 1998. 35 (2): 247- 253.
	Ulrich E , Bernhard G , Stefan H . Recent advances in chlorophyll biobiosynthesis and breakdown in higher plants. Plant Molecular Biology, 2004. 56 (1): 1- 44. doi: 10.1007/s11103-004-2331-3
	Wingler A , Marès M , Pourtau N . Spatial patterns and metabolic regulation of photosynthetic parameters during leaf senescence. New Phytologist, 2004. 161 (3): 781- 789. doi: 10.1111/j.1469-8137.2004.00996.x
	Wu Z M , Zhang X , He B , et al. A chlorophyll-deficient rice mutant with impaired chlorophyllide esterification in chlorophyll biosynthesis. Plant Physiology, 2007. 145 (1): 29- 40. doi: 10.1104/pp.107.100321
	Xia X W , Gui R Y , Yang H Y , et al. Identification of genes involved in color variation of bamboo culms by suppression subtractive hybridization. Plant Physiology Biochemistry, 2015. 97, 156- 164. doi: 10.1016/j.plaphy.2015.10.004
	Ye Z P . A new model for relationship between irriadiance and the rate of photosynthesis in Oryza sativa. Photosynthetica, 2007. 45 (4): 637- 640. doi: 10.1007/s11099-007-0110-5
	Zhang H T , Li J J , Yoo J H , et al. Rice Chlorina-1 and Chlorina-9 encode ChlD and ChlI subunits of Mg-chelatase, a key enzyme for chlorophyll biosynthesis and chloroplast development. Plant Molecular Biology, 2006. 62 (3): 325- 337. doi: 10.1007/s11103-006-9024-z
	Zhu X Y , Guo S , Wang Z W , et al. Map-based cloning and functional analysis of YGL8, which controls leaf colour in rice (Oryza sativa). BMC Plant Biology, 2016. 16 (1): 134. doi: 10.1186/s12870-016-0821-5

表型Phenotypes	Chla/(mg·g^-1 FW)	Chlb/(mg·g^-1 FW)	Chl(a+b)/(mg·g^-1 FW)	Car/(mg·g^-1 FW)
全绿叶片Green leaf(GL)	2.877±0.001a(100)	1.906±0.026a(100)	4.783±0.027a(100)	0.422±0.010a(100)
绿底白纹Green striped leaf(GSL)	2.334±0.032b(81.13)	0.835±0.029b(43.81)	3.169±0.060b(66.26)	0.396±0.002b(93.84)
白底绿纹White striped leaf(WSL)	1.281±0.021c(44.52)	0.417±0.010c(21.88)	1.698±0.031c(35.50)	0.233±0.002c(55.21)
全白叶片White leaf(WL)	0.042±0.004d(1.46)	0.014±0.001d(0.73)	0.056±0.005d(1.17)	0.042±0.004d(9.95)

表型 Phenotype	表观量子效率 AQY	最大净光合速率 P_n-max/ (μmol CO₂·m^-2s^-1)	光饱和点 Light saturation point/(μmol·m^-2s^-1)	光补偿点 Light compensation point/(μmol·m^-2s^-1)	暗呼吸速率 R_d/(μmol CO₂·m^-2s^-1)	校准系数 Adjusted factor (R²)
全绿叶片(GL)	0.07±0.003b(100)	8.05±0.144b(100)	603.66±24.682b(100)	7.63±1.142a(100)	0.57±0.093a(100)	0.999±0.001a
绿底白纹(GSL)	0.08±0.011b(105.41)	5.67±0.092b(70.43)	566.59±29.591b(93.86)	9.77±3.363a(128.05)	0.74±0.318a(129.82)	0.997±0.002a
白底绿纹(WSL)	0.02±0.002a(24.32)	1.00±0.118a(12.42)	431.86±70.416a(71.54)	27.75±3.250b(363.70)	0.54±0.048a (94.74)	0.993±0.032a
全白叶片(WL)	—^①	—	—	—	—	0.327±0.045b

	Chl(a+b) Chlorophyll (a+b)	净光合速率 (P_n)	气孔导度 (G_s)	胞间CO₂浓度 (C_i)	蒸腾速率 (Tr)	水分利用率 (WUE)
Chl(a+b)	1
净光合速率(P_n)	0.827^**	1
气孔导度(G_s)	0.876^**	0.935^**	1
胞间CO₂浓度(C_i)	-0.859^**	-0.917^**	-0.828^**	1
蒸腾速率(Tr)	0.857^**	0.943^**	0.978^**	-0.824^**	1
水分利用率(WUE)	0.755^**	0.832^**	0.698^**	-0.927^**	0.677^**	1

表型 Phenotype	最小荧光值 F_o	PSⅡ原初光能转化效率 F_v/F_m	PSⅡ光化学反应量子效率 Φ_PSⅡ	非光化学猝灭值 q_N	光化学猝灭值 q_P
全绿叶片(GL)	260.67±2.848c(100)	0.82±0.006d(100)	0.70±0.017d(100)	0.25±0.060ab(100)	0.90±0.037a(100)
绿底白纹(GSL)	246.67±4.410c(94.63)	0.73±0.006c(89.02)	0.62±0.009c(88.57)	0.30±0.030ab(118)	0.96±0.014a(106.67)
白底绿纹(WSL)	211.00±4.583b(80.95)	0.61±0.007b(74.39)	0.54±0.021b(77.14)	0.37±0.085b(146.80)	0.91±0.039a(101.11)
全白叶片(WL)	156.67±5.457a(60.10)	0.04±0.004a(4.88)	0.10±0.001a(14.29)	0.10±0.005a(38.00)	0.78±0.061b(86.67)

[1]	刘春洋, 史田, 史国安, 杨林菲, 范学峰, 高双成, 张改娜. 不同移栽时期对‘凤丹’牡丹植株生长效应及其综合评价[J]. 林业科学, 2019, 55(8): 54-62.
[2]	夏国威, 孙晓梅, 陈东升, 张守攻. 日本落叶松冠层光合特性的空间变化[J]. 林业科学, 2019, 55(6): 13-21.
[3]	王怡霖, 王卫锋, 张芸香, 常淑君, 郭晋平. 碧玉杨叶形态结构与生理特性对干旱的响应[J]. 林业科学, 2019, 55(4): 42-50.
[4]	王好运, 吴峰, 朱小坤, 谢维斌. 叶型对马尾松幼苗生长及叶绿素荧光特征的影响[J]. 林业科学, 2019, 55(3): 183-192.
[5]	张韵,刘涛,张涛,谢乐添,黄坚钦,王正加,胡渊渊. 薄壳山核桃果实假果皮的光合特性[J]. 林业科学, 2019, 55(10): 10-18.
[6]	黄亚丽,张军,樊英利,刘易超,杨敏生. 遮荫对中华金叶榆和鑫叶栾叶片呈色及相关生理指标的影响[J]. 林业科学, 2019, 55(10): 171-180.
[7]	种培芳, 詹瑾, 贾向阳, 李毅. 模拟CO₂浓度升高及降雨变化对荒漠灌木红砂光合及生长的影响[J]. 林业科学, 2018, 54(9): 27-37.
[8]	张江涛, 杨淑红, 朱镝, 朱延林, 刘友全. 美洲黑杨2025及其2个芽变品种苗对持续干旱的生理响应及抗旱性评价[J]. 林业科学, 2018, 54(6): 33-43.
[9]	成敏敏, 陈柯伊, 朱雪玉, 王凯利, 周明兵, 杨海芸. 花叶矢竹复绿期光合特性及叶绿体结构[J]. 林业科学, 2018, 54(4): 1-10.
[10]	周艳威, 陈金慧, 鲁路, 成铁龙, 杨立明, 施季森. 杂交鹅掌楸体胚再生植株淹水胁迫下叶片超微结构及光合特性变化[J]. 林业科学, 2018, 54(3): 19-28.
[11]	李威, 杨德光, 牟尧, 杨自超, 王雪蓉, 刘彤彤, 李淑敏. 去遮荫后东北红豆杉幼苗和幼树光合特性对比[J]. 林业科学, 2018, 54(2): 179-185.
[12]	史文辉, 李国雷, 苏淑钗, 刘勇, 贾黎明, 尚治国. 子叶切除与苗圃施肥对栓皮栎容器苗造林效果的影响[J]. 林业科学, 2018, 54(1): 64-73.
[13]	黄绢, 陈存, 张伟溪, 丁昌俊, 苏晓华, 黄秦军. 干旱胁迫对转JERF36银中杨苗木叶片解剖结构及光合特性的影响[J]. 林业科学, 2017, 53(5): 8-15.
[14]	郭文霞, 赵志江, 郑娇, 李俊清. 土壤水分和氮素的交互作用对油松幼苗光合和生长的影响[J]. 林业科学, 2017, 53(4): 37-48.
[15]	徐沁怡, 王标, 赵建文, 吴建峰, 曹亦润, 杨先有, 夏国华, 王正加, 黄坚钦, 胡渊渊. 2种花粉授粉山核桃果皮光合特性的差异比较[J]. 林业科学, 2017, 53(1): 38-46.