林业科学 ›› 2019, Vol. 55 ›› Issue (12): 21-31.doi: 10.11707/j.1001-7488.20191203
陈凌艳1,谢德金2,荣俊冬2,赖金莉1,林雪玲1,郑郁善1,*
收稿日期:
2018-08-19
出版日期:
2019-12-25
发布日期:
2020-01-02
通讯作者:
郑郁善
基金资助:
Lingyan Chen1,Dejin Xie2,Jundong Rong2,Jinli Lai1,Xueling Lin1,Yushan Zheng1,*
Received:
2018-08-19
Online:
2019-12-25
Published:
2020-01-02
Contact:
Yushan Zheng
Supported by:
摘要:
目的: 研究光合色素含量差异对花叶唐竹4种叶色表型光合特性的影响,为花叶竹类的栽培和选育提供参考。方法: 以花叶唐竹2年生分株苗为研究对象,测定全绿、绿底白纹、白底绿纹、全白4种表型叶片的光合色素含量、光合生理参数及叶绿素荧光参数,分别观察绿色叶肉细胞和白色叶肉细胞的超微结构。结果: 4种表型间叶片光合色素含量差异显著,且随着叶片绿色面积比例的下降而呈现显著下降趋势;花叶唐竹绿色叶肉细胞的细胞器排列紧凑且十分完整,叶绿体发育健全,膜系统完善;在大多数白色叶肉细胞中,没有发现完整的叶绿体,而是发育不良的叶绿体和白色体;4种表型叶片的叶绿素含量与其净光合速率、气孔导度、蒸腾速率及水分利用率呈正相关,与胞间二氧化碳浓度呈负相关;全白叶片由于缺乏PSⅡ光反应中心,原初光能转化能力低下,且无法进行光抑制的一系列自我保护反应。结论: 花叶唐竹白色叶肉细胞中叶绿体发育缺陷导致其叶绿素合成受阻,进而影响光合能力,使其净光合速率全为负值,且其自我保护机制较差,强光下易被灼伤,故需要予以适当遮荫。
中图分类号:
陈凌艳,谢德金,荣俊冬,赖金莉,林雪玲,郑郁善. 光合色素含量差异对花叶唐竹不同叶色表型光合特性的影响[J]. 林业科学, 2019, 55(12): 21-31.
Lingyan Chen,Dejin Xie,Jundong Rong,Jinli Lai,Xueling Lin,Yushan Zheng. Effects of Photosynthetic Pigment Content on Photosynthetic Characteristics of Different Leaf Color Phenotypes of Sinobambusa tootsik f. luteoloalbostriata[J]. Scientia Silvae Sinicae, 2019, 55(12): 21-31.
表1
花叶唐竹不同叶片表型光合色素含量①"
表型Phenotypes | Chla/(mg·g-1 FW) | Chlb/(mg·g-1 FW) | Chl(a+b)/(mg·g-1 FW) | Car/(mg·g-1 FW) |
全绿叶片Green leaf(GL) | 2.877±0.001a(100) | 1.906±0.026a(100) | 4.783±0.027a(100) | 0.422±0.010a(100) |
绿底白纹Green striped leaf(GSL) | 2.334±0.032b(81.13) | 0.835±0.029b(43.81) | 3.169±0.060b(66.26) | 0.396±0.002b(93.84) |
白底绿纹White striped leaf(WSL) | 1.281±0.021c(44.52) | 0.417±0.010c(21.88) | 1.698±0.031c(35.50) | 0.233±0.002c(55.21) |
全白叶片White leaf(WL) | 0.042±0.004d(1.46) | 0.014±0.001d(0.73) | 0.056±0.005d(1.17) | 0.042±0.004d(9.95) |
表2
花叶唐竹不同叶色的光合参数"
表型 Phenotype | 表观量子效率 AQY | 最大净光合速率 Pn-max/ (μmol CO2·m-2s-1) | 光饱和点 Light saturation point/(μmol·m-2s-1) | 光补偿点 Light compensation point/(μmol·m-2s-1) | 暗呼吸速率 Rd/(μmol CO2·m-2s-1) | 校准系数 Adjusted factor (R2) |
全绿叶片(GL) | 0.07±0.003b(100) | 8.05±0.144b(100) | 603.66±24.682b(100) | 7.63±1.142a(100) | 0.57±0.093a(100) | 0.999±0.001a |
绿底白纹(GSL) | 0.08±0.011b(105.41) | 5.67±0.092b(70.43) | 566.59±29.591b(93.86) | 9.77±3.363a(128.05) | 0.74±0.318a(129.82) | 0.997±0.002a |
白底绿纹(WSL) | 0.02±0.002a(24.32) | 1.00±0.118a(12.42) | 431.86±70.416a(71.54) | 27.75±3.250b(363.70) | 0.54±0.048a (94.74) | 0.993±0.032a |
全白叶片(WL) | —① | — | — | — | — | 0.327±0.045b |
表3
叶绿素含量与净光合速率、气孔导度、胞间CO2、蒸腾速率及水分利用率之间的相关性分析①"
Chl(a+b) Chlorophyll (a+b) | 净光合速率 (Pn) | 气孔导度 (Gs) | 胞间CO2浓度 (Ci) | 蒸腾速率 (Tr) | 水分利用率 (WUE) | |
Chl(a+b) | 1 | |||||
净光合速率(Pn) | 0.827** | 1 | ||||
气孔导度(Gs) | 0.876** | 0.935** | 1 | |||
胞间CO2浓度(Ci) | -0.859** | -0.917** | -0.828** | 1 | ||
蒸腾速率(Tr) | 0.857** | 0.943** | 0.978** | -0.824** | 1 | |
水分利用率(WUE) | 0.755** | 0.832** | 0.698** | -0.927** | 0.677** | 1 |
表4
花叶唐竹各叶色表型叶绿素荧光参数"
表型 Phenotype | 最小荧光值 Fo | PSⅡ原初光能转化效率 Fv/Fm | PSⅡ光化学反应量子效率 ΦPSⅡ | 非光化学猝灭值 qN | 光化学猝灭值 qP |
全绿叶片(GL) | 260.67±2.848c(100) | 0.82±0.006d(100) | 0.70±0.017d(100) | 0.25±0.060ab(100) | 0.90±0.037a(100) |
绿底白纹(GSL) | 246.67±4.410c(94.63) | 0.73±0.006c(89.02) | 0.62±0.009c(88.57) | 0.30±0.030ab(118) | 0.96±0.014a(106.67) |
白底绿纹(WSL) | 211.00±4.583b(80.95) | 0.61±0.007b(74.39) | 0.54±0.021b(77.14) | 0.37±0.085b(146.80) | 0.91±0.039a(101.11) |
全白叶片(WL) | 156.67±5.457a(60.10) | 0.04±0.004a(4.88) | 0.10±0.001a(14.29) | 0.10±0.005a(38.00) | 0.78±0.061b(86.67) |
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