无机金属盐对微波辅助酸预处理毛竹酶解的影响

doi:10.11707/j.1001-7488.20140415

摘要/Abstract

摘要：

采用加入无机金属盐的稀酸溶液预处理毛竹，分析预处理后底物的化学组成、预处理后的废液组成、糖降解发酵抑制物的生成量等。结果表明：稀酸预处理主要以脱除半纤维素为主，而对木质素的脱除没有明显效果。当硫酸用量为2%（w/w）、预处理温度为180 ℃时，竹材中绝大部分半纤维素已被脱除，底物纤维素转化为葡萄糖的收率可达52.72%。加入硫酸铁催化后的稀酸预处理能提高底物的酶水解性能，可使纤维素转化为葡萄糖的收率提高到72.15%。硫酸钠、硫酸亚铁、硫酸铜和硼砂等催化后的稀酸预处理底物的酶水解性能均有不同程度的下降，其中以硫酸铜催化预处理底物的酶水解性能最差。铜离子在预处理中可以被竹材底物吸附，并且在酶水解过程中可以脱附进入水解液中。

关键词: 毛竹, 酸处理, 金属盐催化, 酶水解

Abstract:

Global warming and energy shortage are the main challenges and puzzles that government and the public around the world are facing. It is believed that one of the solutions is to use biomass as a potential renewable energy resource for producing liquid fuels such as bioethanol and biodiesel. Bamboo, with cellulose and hemicellulose as its main components, is a kind of fast growth and cheap renewable resource for bioethanol production. In the study, inorganic salts were studied as promoter in the dilute acid pretreatment of moso bamboo for the enzymatic saccharification. The chemical composition of the substrates and spent liquors, the enzymatic hydrolyability of substrates were analyzed. The results showed that dilute acid pretreatment (DA) enhances the digestibility of lignocellulose mainly by dissolving hemicellulose and partially prehydrolyzing cellulose. DA has no delignified. The cellulose-to-glucose conversion yield of 2% sulfuric acid(w/w)pretreated substrate was 52.72%. Sulfuric acid with Fe₂(SO₄)₃ pretreated substrate had the higher cellulose-to-glucose conversion yield than sulfuric acid pretreatment. Other metal salts pretreatment efficiency was lower than sulfuric acid pretreatment. And the worst one was CuSO₄. Otherwise, the copper could be absorbed by bamboo during pretreatment and desorption into the hydrolyzate after enzymatic hydrolysis.

Key words: moso bamboo, dilute acid pretreatment, metal salts catalysis, enzymatic hydrolysis

中图分类号:

S785

李志强, 费本华, 江泽慧. 无机金属盐对微波辅助酸预处理毛竹酶解的影响[J]. 林业科学, 2014, 50(4): 101-107.

Li Zhiqiang, Fei Benhua, Jiang Zehui. Effect of Inorganic Salts on Enzymatic Saccharification of Moso Bamboo Pretreated by Microwave-Dilute Acid[J]. Scientia Silvae Sinicae, 2014, 50(4): 101-107.

参考文献

曹金珍. 2006. 国外木材防腐技术和研究现状. 林业科学, 42(7): 120-126.
(Cao J Z. 2006. A review on wood preservation technologies and research. Scientia Silvae Sinicae, 42(7): 120-126. [in Chinese] )
冯国芳, 谢涛, 符向辉, 等. 2008. 酸处理竹笋壳纤维糖化工艺的优化. 湖南工程学院学报, 18(3): 84-86.
(Fen G F, Xie T, Fu X H, et al. 2008. Optimization on saccharification of bamboo shoots shell fibrin (BSSF) by acidic cellulase. Journal of Hunan Institute of Engineering(Natural Science Edition), 18(3): 84-86. [in Chinese] )
国家林业局. 2012. 2012中国林业发展报告. http://www.forestry.gov.cn/portal/main/s/304/content-570738.html.
江泽慧. 2006. 发挥资源优势强化科技创新大才促进林业生物质能源加快发展. 生物质化学工程, (S1): 1-6.
李志强, 江泽慧, 费本华. 2012. 竹材制取生物乙醇原料预处理技术研究进展. 化工进展, 31(3): 533-540.
(Li Z Q, Jiang Z H, Fei B H. 2012. Pretreatment technology of bamboo for bioethanol production:A review. Chemical Industry and Engineering Progress, 31(3): 533-540. [in Chinese] )
岳国君, 武国庆, 郝小明. 2007. 我国燃料乙醇生产技术的现状与展望. 化学进展, 19(7/8): 1084-1090.
(Yue G J, Wu G Q, Hao X M. 2007. The status quo and prospects of fuel ethanol process technology in China. Progress in Chemistry, 19(7/8): 1084-1090. [in Chinese] )
中国纤维素网. 2011. 木质纤维素制备纤维素乙醇是一种趋势.[2011-01-22]. http://www.zgxws.com/news/content-454.htm.
Ballesteros I, Ballesteros M, Manzanares P, et al. 2008. Dilute sulfuric acid pretreatment of cardoon for ethanol production. Biochemical Engineering Journal, 42(1): 84-91.
Davis M W. 1998. A rapid modified method for compositional carbohydrate analysis of lignocellulosics by high pH anion-exchange chromatography with pulsed amperometric detection (HPAEC/PAD). Journal of Wood Chemistry and Technology, 18(2): 235-252.
De Menezes T J B, Dos Santos C L M, Azzini A. 1983. Saccharification of bamboo carbohydrates for the production of ethanol. Biotechnology and Bioengineering, 25(4): 1071-1082.
Dence C W. 1992. The determination of lignin//Dence C W. Methods in lignin chemistry. Springer-Verlag, Berlin, 33-61.
Hendriks A T, Zeeman G. 2009. Pretreatments to enhance the digestibility of lignocellulosic biomass. Bioresource Technology, 100(1): 10-18.
Laluce C, Abud C L, Greenhalf W, et al. 1993. Thermotolerance behavior in sugar cane syrup fermentations of wild type yeast strains selected under pressures of temperature, high sugar and added ethanol. Biotechnology Letters, 15(6): 609-614.
Larsson S, Palmqvist E, Hahn-Hägerdal B, et al. 1999. The generation of fermentation inhibitors during dilute acid hydrolysis of softwood. Enzyme Microb Technol, 24(3/4): 151-159.
Leenakul W, Tippayawong N. 2010. Dilute acid pretreatment of bamboo for fermentable sugar production. Journal of Sustainable Energy & Environment, 1(3): 117-120.
Li Z Q, Li X W, Zhang Y, et al. 2009. Fixation property of copper triazole wood preservatives. Chinese Forestroy Science and Technology, 8(1): 15-23.
Liu Z L, Slininger P J, Dien B S, et al. 2004. Adaptive response of yeasts to furfural and 5-hydroxymethylfurfural and new chemical evidence for HMF conversion to 2, 5-bis-hydroxymethylfuran. Journal of Industrial Microbiology and Biotechnology, 31(8): 345-352.
Nguyen Q A, Tucker M P, Keller F A, et al. 1999. Dilute acid hydrolysis of softwoods. Applied Biochemistry and Biotechnology, 77(1/3): 133-142.
Shuai L, Yang Q, Zhu J Y, et al. 2010. Comparative study of SPORL and dilute-acid pretreatments of spruce for cellulosic ethanol production. Bioresource Technology, 101(9): 3106-3114.
Sluiter A, Hames B, Ruiz R, et al. 2008. Laboratory analytical procedure (LAP): determination of structural carbohydrates and lignin in biomass. Technical Report NREL/TP-510-42618. http://www.nrel.gov/biomass/analytical_procedures.html.

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