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林业科学 ›› 2014, Vol. 50 ›› Issue (4): 101-107.doi: 10.11707/j.1001-7488.20140415

• 论文与研究报告 • 上一篇    下一篇

无机金属盐对微波辅助酸预处理毛竹酶解的影响

李志强, 费本华, 江泽慧   

  1. 国际竹藤中心 北京 100102
  • 收稿日期:2013-06-24 修回日期:2013-10-10 出版日期:2014-04-25 发布日期:2014-05-06
  • 基金资助:

    国家“十二五”科技支撑计划项目(2012BAD54G01)。

Effect of Inorganic Salts on Enzymatic Saccharification of Moso Bamboo Pretreated by Microwave-Dilute Acid

Li Zhiqiang, Fei Benhua, Jiang Zehui   

  1. International Centre for Bamboo and Rattan Beijing 100102
  • Received:2013-06-24 Revised:2013-10-10 Online:2014-04-25 Published:2014-05-06
  • Contact: 江泽慧

摘要:

采用加入无机金属盐的稀酸溶液预处理毛竹,分析预处理后底物的化学组成、预处理后的废液组成、糖降解发酵抑制物的生成量等。结果表明:稀酸预处理主要以脱除半纤维素为主,而对木质素的脱除没有明显效果。当硫酸用量为2%(w/w)、预处理温度为180 ℃时,竹材中绝大部分半纤维素已被脱除,底物纤维素转化为葡萄糖的收率可达52.72%。加入硫酸铁催化后的稀酸预处理能提高底物的酶水解性能,可使纤维素转化为葡萄糖的收率提高到72.15%。硫酸钠、硫酸亚铁、硫酸铜和硼砂等催化后的稀酸预处理底物的酶水解性能均有不同程度的下降,其中以硫酸铜催化预处理底物的酶水解性能最差。铜离子在预处理中可以被竹材底物吸附,并且在酶水解过程中可以脱附进入水解液中。

关键词: 毛竹, 酸处理, 金属盐催化, 酶水解

Abstract:

Global warming and energy shortage are the main challenges and puzzles that government and the public around the world are facing. It is believed that one of the solutions is to use biomass as a potential renewable energy resource for producing liquid fuels such as bioethanol and biodiesel. Bamboo, with cellulose and hemicellulose as its main components, is a kind of fast growth and cheap renewable resource for bioethanol production. In the study, inorganic salts were studied as promoter in the dilute acid pretreatment of moso bamboo for the enzymatic saccharification. The chemical composition of the substrates and spent liquors, the enzymatic hydrolyability of substrates were analyzed. The results showed that dilute acid pretreatment (DA) enhances the digestibility of lignocellulose mainly by dissolving hemicellulose and partially prehydrolyzing cellulose. DA has no delignified. The cellulose-to-glucose conversion yield of 2% sulfuric acid(w/w)pretreated substrate was 52.72%. Sulfuric acid with Fe2(SO4)3 pretreated substrate had the higher cellulose-to-glucose conversion yield than sulfuric acid pretreatment. Other metal salts pretreatment efficiency was lower than sulfuric acid pretreatment. And the worst one was CuSO4. Otherwise, the copper could be absorbed by bamboo during pretreatment and desorption into the hydrolyzate after enzymatic hydrolysis.

Key words: moso bamboo, dilute acid pretreatment, metal salts catalysis, enzymatic hydrolysis

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