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林业科学 ›› 2018, Vol. 54 ›› Issue (1): 99-104.doi: 10.11707/j.1001-7488.20180111

• 论文与研究报告 • 上一篇    下一篇

基于拉曼光谱技术的竹材细胞壁化学组分分布

韦鹏练, 杨淑敏, 刘嵘, 安鑫, 费本华   

  1. 国际竹藤中心 北京 100102
  • 收稿日期:2016-05-10 修回日期:2016-08-17 出版日期:2018-01-25 发布日期:2018-03-01
  • 基金资助:
    国家自然科学基金面上项目"竹纤维细胞壁多层构造的理化表征"(31370563)。

Analysis of Chemical Constituents Distribution of Moso Bamboo Fiber Cell Wall Based on Raman Spectra

Wei Penglian, Yang Shumin, Liu Rong, An Xin, Fei Benhua   

  1. International Center for Bamboo and Rattan Beijing 100102
  • Received:2016-05-10 Revised:2016-08-17 Online:2018-01-25 Published:2018-03-01

摘要: [目的]研究竹纤维单细胞中重要化学组分的分布,揭示竹纤维细胞壁中化学组分的分布规律。[方法]以6年生毛竹为研究对象,取竹壁中部竹材,制成超薄切片,利用高分辨率激光共聚焦拉曼显微镜对纤维细胞进行原位光谱采集,通过光谱成像技术分析毛竹纤维单细胞中重要化学组分的分布。[结果]S型木质素和G型木质素广泛分布于细胞壁各壁层中,而H型木质素则主要分布在细胞外缘。羟基肉桂酸的分布与H型木质素类似,也主要存在于细胞外缘的壁层中。纤维素在细胞壁宽壁层中的分布相对稳定,且比窄壁层具有更高的分布密度;从细胞外围向细胞内部,木质素的分布密度总体呈下降趋势,在复合胞间层、细胞腔周围以及各壁层之间的交界处还具有相对较高的分布密度,形成局部聚集分布。[结论]竹纤维细胞壁径向方向具有不同的化学组成,纤维素、木质素以及羟基肉桂酸在不同壁层之间具有不同的分布密度。

关键词: 细胞壁, 毛竹, 纤维, 拉曼光谱, 化学成分

Abstract: [Objective] In order to reveal chemical constituents distribution rule of bamboo fiber cell wall, distribution of some important chemicals within single fiber cell was studied.[Method] Six-year-old moso bamboo (Phyllostachys edulis) was chosen and sampled into ultrathin section as research material. High-resolution confocal Raman microscopy was used to collect Raman spectrum of bamboo fiber in situ. The chemical constituents distribution of moso bamboo fiber cell wall was analyzed through Raman imaging technique.[Result] S unit and G unit of lignin widely distributed in all layers of wood cell wall, but H unit mainly distributed in layers that close to peripheral region. The distribution pattern of hydroxycinnamic acids was similar to that of H unit, i.e. mainly distributed in peripheral region of cell wall. Cellulose had a relatively even distribution in thick layers, and the concentration of cellulose was higher in thick layers than that of thin layers. Lignin concentration decreased from periphery to central part of a fiber cell, with relative higher value at compound middle lamella, lumen edge as well as interface between two layers which formed local accumulation at those regions.[Conclusion] The bamboo fiber cell wall has different chemical composition in radial direction, and cellulose, lignin and hydroxycinnamic acid have different distribution density within different cell wall layers.

Key words: cell wall, Phyllostachys edulis, fiber, Raman spectrum, chemical component

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