林业科学 ›› 2007, Vol. 43 ›› Issue (10): 150-153.doi: 10.11707/j.1001-7488.20071028
• 研究简报 • 上一篇
吴松权1 管清杰2 全雪丽1 傅伟杰1 吴基日1
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Wu Songquan1 Guan Qingjie2 Quan Xueli1 Fu Weijie1 Wu Jiri1
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关键词: 松茸, 假松茸, RAPD, SCAR
Abstract:
RAPD analysis was adopted to identify the Tricholoma matsutake and T. bakamatsutake. With the method of BSA, a polymorphic band of 870 bp linked to T. matsutake was amplified, and the SCAR marker was developed from the polymorphic band by cloning, sequencing and designing primers. The SCAR marker only presented in the individuals of T. matsutake, thus, it can quickly and accurately identify and T. bakamatsutake at molecular level.
Key words: Tricholoma matsutake, Tricholoma bakamatsutake, RAPD, SCAR
吴松权 管清杰 全雪丽 傅伟杰 吴基日. 松茸SCAR标记的建立[J]. 林业科学, 2007, 43(10): 150-153.
Wu Songquan Guan Qingjie Quan Xueli Fu Weijie Wu Jiri. Establishment of SCAR Marker Linked to Tricholoma matsutake[J]. Scientia Silvae Sinicae, 2007, 43(10): 150-153.
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