银杏、杉木、杨树叶中磷脂酰甘油分子种的高效液相色谱分析

摘要/Abstract

摘要：

用高效液相色谱分析方法,在碳十八柱上,以乙腈-异丙醇为流动相,分析了银杏、杉木、杨树叶中磷脂酰甘油的分子种组成。分析采用磷脂酰甘油经磷脂酶C水解后,用3, 5-二硝基苯甲酰氯衍生,再进行高效液相色谱分离的方法。分子种根据文献的相对保留值、标样的实验保留值、分析样HPLC峰的脂肪酸组成分析而确定。分析结果表明,三种林木叶中磷脂酰甘油均主要由八个分子种组成,在八个分子种的相对含量上,树种间存在明显差异。

关键词: 银杏, 杉木, 杨树, 磷脂酰甘油, 分子种, 高效液相色谱

Abstract:

Molecular species of phosphatidylglycerol in leaves of Ginkgo biloba, Chinese fir and Poplar were analyzed by HPLC on a C₁₈ column with acetonitoile-2-propanol (80 : 20, v / v) as mobile phase. 8 molecular species of PG were found in these 3 trees, they are (18:3/16:1〈3t〉)PG. (18:3/16:0)PG. (18:2/16:1〈3t〉)PG. (18:2/16:0)PG. (18:1/16:1〈3t〉PG, (16:0 /16:1〈3t〉PG, (18:1/16:0)PG, (16:0/16:0)PG, The relative amounts of these 8 molecular species in 3 bees are significantly different: (mol%) Ginkgo biloba, 30.5, 12.6, 1 7.9, 22.4, 0.2, 5.1, 4.0, 5.3, Chinese fir, 6.4, 16.1, 1 1.5, 33.2, 0.3, 3.0, 19.8, 7.3, Poplar, 10.1, 10.6, 10.6, 14.1, 8.4, 22.3, 12.5, 11.3.

Key words: Ginkgo biloba, Chinese fir, Poplar, Phosphatiylglycerol, Molecular species, High performance liquid chromatography (HPLC)

包宏,沈漫,夏民洲,胡兹苓. 银杏、杉木、杨树叶中磷脂酰甘油分子种的高效液相色谱分析[J]. 林业科学, 1997, 33(5): 447-453.

Hong Bao,Man Shen,Minzhou Xia,Ziling Hu. HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) ANALYSIS OF MOLECULAR SPECIES OF PHOSPHATIDYLGLYCEROL FROM LEAVES OF GINKGO BILOBA, CHINESE FIR AND POPLAR[J]. Scientia Silvae Sinicae, 1997, 33(5): 447-453.

图/表 4

参考文献 16

1	胡兹苓, 包宏, 郭会玲. 杨树膜脂脂肪酸和磷脂的组成与抗寒性的关系. 林业科学, 1993. (6): 543- 546.
2	包宏, 关世英, 胡兹苓, 等. 零下低温对杂交杨树皮层膜脂组成的影响. 植物资源与环境, 1994. 3 (4): 39- 43.
3	关世英, 苏维埃. 与磷脂酰甘油有关的植物抗冷机理研究进展. 植物生理学通讯, 1995. 31 (3): 167- 173.
4	苏维埃, 王文英, 李锦树. 植物类脂及其脂肪酸的分析技术. 植物生理学通讯, 1980. (3): 54- 60.
5	包宏, 李梅, 胡兹苓, 等. 杉木针叶磷脂酰甘油脂肪酸组成分析. 色谱, 1997. 15 (2): 125.
6	Kasamo K , Kagita F , Yamanishi , et al. Low temperature-induced changes in the thermotoopic properties and fatty acid composition of the plasma membrane and tonoplast of cultured nice cells. Plant cell Physiol., 1992. 33 (5): 609.
7	Roughan P , Holland R , Slack C . The role of chloroplasts and microsomal fractions in polar-lipid synthesis from acetate by cell-free preparations from spinach leaves. Biochem. J., 1980. 17, 188.
8	Tasaka Yasushi , Nishida Ikwo , Higashi Shoichi , et al. Fatty acid. composition of phosphatidylglycerols in relation to chilling sensitivity of woody plants. Plant Cell Physiol., 1990. 31 (4): 545- 550.
9	Frentzen M, Heinz E, Mekeon A, Properties of the plastidial acyl-(Acyl-Carrier -Protein): glycerol-3-phosphate acyltransferase from the chilling-sensitive plant squash. Plant Cell Physiol., 1987, 28(7): 1195
10	Murata N , Sato N , Takahashi N , et al. Composition and positional distributions of fatty acids in phospholipids from leaves of chilling-sensitive and chilling-resistant plants. Plant Cell Physiol., 1982. 23 (6): 1071.
11	Murata N , Ishizaki O , Higashi S , et al. Genetically engineered alteration in the chilling sensitivity of plants. Nature, 1992. 356, 710- 713.
12	Makoto K , Hitoshi. T. , Hiroshi N. , et al. A sensitive method for quantitative analysis of phospholipid molecular species by high performance Liquid chromatographs. J. Biochem., 1985. 98, 327- 331.
13	Jiro S , Katumasa N , Noboru S . Molecular species of phosphatidylglycerol from leaves of various citrus species. Agric. Biol. Chem., 1990. 54 (11): 2777- 2782.
14	Jiro S , Kazuhiko Y , Noboru S . Molecular species of phospho lipids of lilium iongiflorum pollen. Agnic. Biol. chem., 1990. 54 (1): 191- 195.
15	Hu Ziling , Fazil O , Bao Hong , et al. Composition and positional distibution of fatty acids in leaf phospholipids. Archives des Sciences, 1996. 49 (1): 11- 20.
16	Murata N . Molecular species composition of phosphatidylglycerols from chilling-sensitive and chilling resistant plants. Plant Cell Physiol., 1983. 24 (1): 81- 86.

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