杨树PagABP1介导生长素信号影响植株生长

doi:10.11707/j.1001-7488.LYKX20250329

摘要/Abstract

摘要：

目的: 对84K杨PagABP基因家族进行全基因组鉴定和生物信息学分析，选择在顶芽、幼茎、形成层、韧皮部和木质部均高表达的PagABP1基因，探究该基因对杨树径向生长和高生长的调控作用，为阐明ABP1介导的生长素信号通路在茎生长中的分子机制提供参考。方法: 采用生物信息学相关方法，鉴定84K杨PagABP基因家族成员；利用qRT-PCR技术，分析PagABP1基因在顶芽、幼叶、幼茎、幼根、成熟茎、老根、根尖、韧皮部、维管形成层和木质部中的表达；分别将PagABP1启动子和编码区序列构建植物P_PagABP1::GUS启动子载体和过表达35S::PagABP1载体，应用农杆菌介导法创制84K杨转基因材料，探究PagABP1基因对杨树径向生长和高生长的影响。结果: 在84K杨基因组中鉴定到2个ABP基因家族成员，其中PagABP1基因在顶芽、幼茎、形成层、韧皮部和木质部中相对表达量较高；P_pagABP1::GUS转基因杨与P_pagDR5::GUS（生长素响应报告基因）转基因杨基因表达位置重合，主要表达于顶芽、幼茎、形成层、韧皮部与初生木质部，提示PagABP1参与生长素诱导影响径向生长和高生长；截顶土栽苗在萌芽数和生长速率上，PagABP1-OE株系均显著大于对照；分析60天的过表达PagABP1基因的转基因株系（#17、#26）发现，第12茎节间形成层宽度、初生木质部宽度、韧皮部宽度、株高和地径分别较对照提高50.2%、43.3%、1.58%、19.14%和19.29%。结论: PagABP1基因与生长素表达部位关联，主要在顶芽、幼茎、形成层、韧皮部和初生木质部中表达，影响杨树径向生长和高生长。

关键词: 84K杨, 生长素信号, PagABP1, 径向生长, 高生长

Abstract:

Objective: Through genome-wide identification and bioinformatics analysis of PagABP gene family of Populus alba × P. glandulosa, the gene PagABP1 that is highly expressed in young stems, cambium, xylem and phloem was selected to explore its effect on radial and height growth of this poplar. This study aims to provide a theoretical basis for elucidating how the auxin signaling pathway regulates secondary growth of stems by studying the regulatory role of this gene in the radial and height growth of poplars. Method: Bioinformatics and related software were used to identify the members of the PagABP gene family in 84K poplar. The real-time fluorescence quantitative PCR was used to analyze the expression of PagABP1 gene in terminal buds, young leaves, young stems, young roots, mature stems, old roots, root tips, phloem, vascular cambium and xylem. Plant P_PagABP1::GUS promoter vector and overexpressing 35S::PagABP1 vector were constructed by using the PagABP1 promoter and coding region sequences, respectively. Agrobacterium-mediated method was used to create 84K poplar transgenic materials that were used to identify the effects of PagABP1 gene on poplar radial and height growth. Result: Two ABP homologous genes were identified in 84K poplar genome, and the PagABP1 gene showed relatively higher expression levels in the apical bud, young stems, phloem, cambium, and xylem. The expression locations of P_PagABP1::GUS in transgenic poplar were overlapped with those of P_pagDR5::GUS (auxin response reporter gene), mainly in the terminal bud, young stem, cambium, phloem, and primary xylem, suggesting that PagABP1 is involved in auxin-mediated regulation of radial and height growth. The germination number and growth rate of PagABP1-OE lines were significantly higher than those of the control when the truncated seedlings were planted in soil. Analysis of transgenic lines (#17、#26) that overexpressed PagABP1 gene at 60 days showed that the width of the cambium, primary xylem, and phloem of 12th internode, plant height and basal diameter increased by 50.2%, 43.3%, 1.58%, 19.14% and 19.29%, respectively, compared to the control. Conclusion: PagABP1 gene is associated with auxin expression site, mainly expressed in terminal buds, young stem, phloem, cambium, and primary xylem, affecting radial and height growth. This study can lay a foundation for further revealing the molecular mechanism of PagABP1 gene involved in stem growth of 84K poplar.

Key words: Populus alba × P. glandulosa, auxin signaling, PagABP1, radial growth, height growth

中图分类号:

S718.46

王深,曾智新,乔静,王雨馨,郑瑞琦,王启,吴荟,张琦琦,焦阳,舒文波. 杨树PagABP1介导生长素信号影响植株生长[J]. 林业科学, 2026, 62(2): 126-133.

Shen Wang,Zhixin Zeng,Jing Qiao,Yuxin Wang,Ruiqi Zheng,Qi Wang,Hui Wu,Qiqi Zhang,Yang Jiao,Wenbo Shu. PagABP1 in Populus Mediates Auxin Signaling to Influence Plant Growth[J]. Scientia Silvae Sinicae, 2026, 62(2): 126-133.

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引物名称 Primer name	序列（5'–3'） Sequences（5'–3'）
PagABP1	AGCGGTGTGCTTGTGCTC
PagABP1	CAAACCTCCCCTTCCATA
PagUBQ	GTTGATTTTTGCTGGGAAGC
PagUBQ	GATCTTGGCCTTCACGTTGT

引物名称 Primer name	引物序列 Primer sequence（5'–3'）
ABP1-Pro-F	TTTAATGGAAACCCGTAGTCT
ABP1-Pro-R	AAATGAGCACAAGCACAC
PMV2-JD-F（菌落鉴定Colony identification）	TATGACCATGATTACGCCAAGC
PMV2-F（同源臂Homologous arm）	TGCATCCAACGCGTTGGGAGCTC
PMV2-R（同源臂Homologous arm）	GCCTTCGCCATTCTAGACTCGAG
ABP1-CDS-F	ATGAGGAGGATGAGCGGTGT
ABP1-CDS-R	TCAAAGTTCATCTTTTAGAGG
2301-JD-R（菌落鉴定Colony identification）	TATGACCATGATTACGAATTCGG
2301-F（同源臂Homologous arm）	AGCTTTCGCGAGCTCGGTACC
2301-R（同源臂Homologous arm）	TCTAGAGGATCCCCGGGTACC

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