不同密度杂种落叶松人工林土壤细菌群落对凋落物分解过程的响应

doi:10.11707/j.1001-7488.20131215

林业科学 ›› 2013, Vol. 49 ›› Issue (12): 101-107.doi: 10.11707/j.1001-7488.20131215

不同密度杂种落叶松人工林土壤细菌群落对凋落物分解过程的响应

林英华¹, 汪来发², 卢萍^1,2, 谭飞^1,3, 徐演鹏^1,3, 孙楠⁴

1. 中国林业科学研究院湿地研究所北京 100091;
2. 中国林业科学研究院森林生态环境与保护研究所国家林业局森林保护学重点实验室北京 100091;
3. 东北林业大学野生动物资源学院哈尔滨 150040;
4. 黑龙江省林业科学院哈尔滨 150081

收稿日期:2012-11-06 修回日期:2013-07-29 出版日期:2013-12-25 发布日期:2014-01-01
基金资助:
国家自然科学基金项目（31071904）；国家科技支撑计划项目（2012BAD24B06）。

Response of Soil Bacteria Community to Leaf Litter Decomposition of Larch (Larix olgensis×Larix kaempferi) Plantation with Different Forest Densities

Lin Yinghua¹, Wang Laifa², Lu Ping^1,2, Tan Fei^1,3, Xu Yanpeng^1,3, Sun Nan⁴

1. Institute of Wetland Research, CAF Beijing 100091;
2. Key Laboratory of Forest Protection of State Forestry Administration Research Institute of Forest Ecology, Environment and Protection, CAF Beijing 100091;
3. College of Wildlife Resource, Northeast University of Forestry Harbin 150040;
4. Heilongjiang Academy of Forestry Harbin 150081

Received:2012-11-06 Revised:2013-07-29 Online:2013-12-25 Published:2014-01-01

摘要/Abstract

摘要：

应用稀释平板法和16S RNA序列分析法对不同密度杂种落叶松长白落叶松又日本落叶松人工林2种孔径凋落袋，即上层网孔2 mm、下层网孔1 mm的异孔分解袋和网孔均为1/300 mm的同孔分解袋中凋落物分解过程中土壤细菌群落及多样性进行研究。结果表明：土壤细菌数量因凋落物分解而发生明显变化（F=10.97，P<0.01），其中以芽孢杆菌、假单胞菌、葡萄球菌为代表的纤维素、半纤维素分解菌数量最多。2种孔径凋落袋下菌落的变化不一致：同孔分解袋中菌落随造林密度加大而增多，优势现象明显；异孔分解袋中菌落数随造林密度增加而减少，优势现象不显著。土壤细菌的组成与多样性主要受采样时段影响。不同密度杂种落叶松林土壤细菌群落具有很高的相似性。造林密度对2种凋落袋分解过程中土壤细菌群落的多样性与复杂性影响不一致。典型相关分析表明：土壤细菌群落生存的环境条件较为相似，土壤K对土壤细菌分布影响最大，土壤C/N对土壤细菌分布影响最小。

关键词: 落叶松, 细菌群落多样性, 凋落物分解袋, 动态变化, 土壤性质, 典型相关分析

Abstract:

Soil bacteria community and their diversity were studied during the leaf litter decomposition of Larix olgensis× Larix kaempferi plantation with different densities by two mesh size of litterbags, that is, top layer of 2 mm mesh and bottom layer of 1 mm mesh (mixed mesh litterbag), as well as 1/300 mm mesh size(same mesh litterbag)of litterbags. The bacteria were investigated with the plate cultivation method and PCR-16S RNA sequence analysis. The result showed that the number of soil bacteria was changed significantly (F=10.97, P<0.01) during leaf litter decomposition. The bacteria participating in cellulose, hemicellulose decomposition, such as Bacillus, Pseudomonas, Staphylococcus, was the most dominant. The variety of bacteria community was not consistent between the two types of litterbags. The number of bacteria in the same mesh litterbag increased with the plantation density and some species were obviously dominated, while the number of bacteria in the mixed mesh litterbag decreased with the plantation density and there were no significant dominant species. The composition and diversity of soil bacteria community were mainly influenced by the sampling period. Soil bacteria communities were similar among the different densities of Larix olgensis×Larix kaempferi plantation. The plantation density had different effect on soil bacterial diversity and complexity during leaf litter decomposition of two types of litterbags. The canonical correlation analysis showed that the environmental conditions suitable to soil bacterial community were similar. Soil potassium had the most effect on distribution of soil bacteria, while the ratio of soil carbon to nitrogen had the least effect.

Key words: larch, soil bacteria community diversity, litterbag, dynamic, soil property, canonical correspondence analysis

中图分类号:

S718.8

林英华, 汪来发, 卢萍, 谭飞, 徐演鹏, 孙楠. 不同密度杂种落叶松人工林土壤细菌群落对凋落物分解过程的响应[J]. 林业科学, 2013, 49(12): 101-107.

Lin Yinghua, Wang Laifa, Lu Ping, Tan Fei, Xu Yanpeng, Sun Nan. Response of Soil Bacteria Community to Leaf Litter Decomposition of Larch (Larix olgensis×Larix kaempferi) Plantation with Different Forest Densities[J]. Scientia Silvae Sinicae, 2013, 49(12): 101-107.

参考文献

柴立元, 陈跃辉, 黄燕, 等. 2010. 三国吴简蚀斑可培养微生物的多样性. 中南大学学报:自然科学版, 41 (5): 1674-1679.
陈华癸, 李阜康, 陈文新, 等. 1981. 土壤微生物学. 上海: 上海科学技术出版社, 10-12.
陈声明, 林海萍, 张立钦. 2007. 微生物学导论. 北京: 高等教育出版社.
陈祥伟, 魏占才, 曹文英, 等. 1995. 张广才岭西坡人工针叶林小气候效应的研究. 东北林业大学学报, 23 (5): 29-36.
东秀珠, 蔡妙英. 2001. 常见细菌系统鉴定手册. 北京: 科学出版社.
郭继勋, 祝廷成. 1993. 羊草草原枯落叶分解的研究. 生态学报, 13 (3): 214-219.
林英华, 孙家宝, 郑桂华, 等. 2005. 帽儿山土壤动物在凋落叶分解过程中的动态和作用. 东北林业大学学报, 33(6): 33-36.
孙楠, 李亚洲, 吴瑶. 2012. 造林密度对杂种落叶松人工林凋落物及其分解速率的影响. 黑龙江生态工程职业学院学报, 25 (3): 17-18.
孙楠, 于秀兰, 张怡春, 等. 2009. 造林密度对杂种落叶松生长影响的初步分析. 吉林林业科技, 38(2): 1-4.
王树力, 孙悦, 沈海燕, 等. 2009. 不同密度杂种落叶松人工林的土壤微生物变化特征. 中国水土保持科学, 7 (3): 59-66.
Cao Y, Fu S, Zou X, et al. 2010. Soil microbial community composition under eucalyptus plantations of different age in subtropical China. European Journal of Soil Biology, 46: 128-135.
Chen S, Steinberger Y. 2012. Microbial functional diversity associated with plant litter decomposition along a climatic. Gradient Microbial Ecology, 64: 399-415.
Cornwell W K, Cornelissen J H C, Amatangelo K, et al. 2008. Plant species traits are the predominant control on litter decomposition rates within biomes worldwide. Ecology Letter, 11: 1065-1071.
Fraterrigo J M, Balser T C, Turner M G. 2006. Microbial community variation and its relationship with nitrogen mineralization in historically altered forests. Ecology, 87: 570-579.
Janssen P H, Yates P S, Grinton B E, et al. 2002. Improved culturability of soil bacteria and isolation in pure culture of novel members of the divisions Acidobacteria, Actinobacteria, Proteobacteria and Verrucomicrobia. Applied and Environmental Microbiology, 68: 2391-2396.
Kawanishi T, Shiraishi T, Okano Y, et al. 2011. New detection systems of bacteria using highly selective media designed by SMART: Selective medium-design algorithm restricted by two constraints. PLoS ONE, 6(1): e16512.
Lee D, Noh S, Kim C. 2000. Development of molecular biological methods to analyze bacterial species diversity in fresh water and soil ecosystems. Journal of Microbiology, 38(1): 11-17.
McCaig A E, Grayston S J, Prosser J I, et al. 2001. Impact of cultivation on characterisation of species composition of soil bacterial communities. FEMS Microbiology Ecology, 35: 37-48.
Melillo J M, Aber J D, Muratore F. 1982. Nitrogen and lignin control of hardwood leaf litter decomposition dynamics. Ecology, 63: 621-626.
Nannipieri P, Ascher J, Ceccherini M T, et al. 2003. Microbial diversity and soil functions. European Journal of Soil Science, 54: 655-670.
Schinner F. 1982. Soil microbial activities and litter decomposition related to altitude. Plant and Soil, 65: 87-94.
Seo J, Keum Y, Li Q X. 2009. Bacterial degradation of aromatic compounds. International Journal of Environmental Research and Public Health, 6: 278-309.

[1]	丁令智, 满秀玲, 肖瑞晗, 蔡体久. 寒温带森林根际土壤微生物量碳氮含量生长季内动态变化[J]. 林业科学, 2019, 55(7): 178-186.
[2]	夏国威, 孙晓梅, 陈东升, 张守攻. 日本落叶松冠层光合特性的空间变化[J]. 林业科学, 2019, 55(6): 13-21.
[3]	吕振刚, 李文博, 黄选瑞, 张志东. 气候变化情景下河北省3个优势树种适宜分布区预测[J]. 林业科学, 2019, 55(3): 13-21.
[4]	Shahzad Muhammad, 韩斐斐, 姜立春. 不同抽样方法对兴安落叶松立木材积方程预测精度的影响[J]. 林业科学, 2018, 54(8): 99-105.
[5]	王丽群, 张志强, 李格, 马丰伟, 陈立欣. 北京边缘地区人居林结构、动态变化及生态效益初步分析——以牛栏山-马坡镇为例[J]. 林业科学, 2018, 54(8): 142-152.
[6]	宋跃, 李淑娟, 张含国, 白晓明, 毕显禹, 董实伟, 董昊. 落叶松胚性愈伤组织悬浮培养体系的优化[J]. 林业科学, 2018, 54(7): 146-154.
[7]	李梁, 张建军, 陈宝强, 冯换成, 张守红, 孙若修, 高思远, 张海博, 印家齐. 晋西黄土区长期封禁小流域植被群落动态变化[J]. 林业科学, 2018, 54(2): 1-9.
[8]	马岩岩, 姜立春. 异速生长模型的误差结构和误差函数[J]. 林业科学, 2018, 54(2): 90-97.
[9]	王滋, 周贤武, 武国芳, 钟永, 任海青, 赵荣军. 国产落叶松平行弦轻型木桁架静力承载性能[J]. 林业科学, 2018, 54(2): 137-144.
[10]	冯宜明, 李毅, 曹秀文, 刘锦乾, 齐瑞, 赵阳, 陈学龙. 甘肃南部不同密度云杉人工幼林的林分结构特征及土壤理化性质[J]. 林业科学, 2018, 54(10): 20-30.
[11]	姜礅, 薛羿, 徐智文, 王嘉冰, 孟昭军, 严善春. 喷施茉莉酸诱导长白落叶松抗性对舞毒蛾生长发育的影响[J]. 林业科学, 2018, 54(1): 162-167.
[12]	王立祥, 刘晓博, 任利利, 石娟, 骆有庆. 松树蜂入侵的混交林中针叶树种内生真菌多样性[J]. 林业科学, 2017, 53(9): 81-89.
[13]	王艳兵, 王彦辉, 熊伟, 姚依强, 张桐, 李振华. 六盘山半干旱区华北落叶松树干液流速率及主要影响因子的坡位差异[J]. 林业科学, 2017, 53(6): 10-20.
[14]	顾伟, 马玲, 孙虎, 王利东, 张子龙. 不同土壤环境下落叶松人工林昆虫群落结构及动态的差异[J]. 林业科学, 2017, 53(5): 97-106.
[15]	高慧淋, 董利虎, 李凤日. 基于混合效应的人工落叶松树冠轮廓模型[J]. 林业科学, 2017, 53(3): 84-93.

不同密度杂种落叶松人工林土壤细菌群落对凋落物分解过程的响应

Response of Soil Bacteria Community to Leaf Litter Decomposition of Larch (Larix olgensis×Larix kaempferi) Plantation with Different Forest Densities

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

作者中心

期刊获奖

引证指标

联系方式