钾胁迫条件下Ca2+信号抑制剂对外生菌根真菌分泌H+的影响

doi:10.11707/j.1001-7488.20131214

林业科学 ›› 2013, Vol. 49 ›› Issue (12): 97-100.doi: 10.11707/j.1001-7488.20131214

钾胁迫条件下Ca²⁺信号抑制剂对外生菌根真菌分泌H⁺的影响

郭涛^1,2, 戴景程¹, 朱敏¹

1. 西南大学资源环境学院重庆 400716;
2. 国家紫色土土壤肥力与肥料效益监测基地重庆 400716

收稿日期:2012-11-01 修回日期:2013-10-05 出版日期:2013-12-25 发布日期:2014-01-01

Effects of Ca²⁺ Signal Inhibitors on H⁺ Effluxes by Ectomycorrhizal Fungi under Potassium Stress

Guo Tao^1,2, Dai Jingcheng¹, Zhu Min¹

1. College of Resources and Environment, Southwest University Chongqing 400716;
2. National Monitoring Base for Purple Soil Fertility and Fertilizer Efficiency Chongqing 400716

Received:2012-11-01 Revised:2013-10-05 Online:2013-12-25 Published:2014-01-01

摘要/Abstract

摘要：

为了研究钾胁迫条件下外生菌根真菌分泌H⁺的调控机制，以从强酸性森林土壤中分离获得的菌株褐环乳牛肝菌（Sl01）、牛肝菌（Bo08）以及土生空团菌（CgSIV）为材料，采用液体培养方法，研究低钾胁迫下外生菌根真菌的生长与H⁺分泌的关系，以及Ca²⁺信号抑制剂对H⁺分泌的影响。结果表明：在正常供钾条件下，3种菌株的生长与H⁺的分泌未受到Ca²⁺信号抑制剂的显著影响；而在低钾条件下，Ca²⁺信号抑制剂三氟拉嗪（TFP）和钙离子螯合剂EGTA都显著减少菌株Bo08和Sl01的H⁺分泌，即在此试验条件下，Bo15和Sl08的H⁺分泌均受到Ca²⁺信号转导的调控，说明在低钾胁迫条件下，Ca²⁺信号是介导外生菌根真菌分泌H⁺的信号因子。

关键词: 钾, 钙调蛋白抑制剂, 外生菌根真菌

Abstract:

In this study, Suillus luteus (Sl01) and Boletus sp. (Bo08), isolated from the acidic forest soil, and Cenococcum geophilum (CgSIV) were cultured in liquid media with various potassium concentrations and Ca²⁺ signal inhibitors to investigate mechanism of H⁺ effluxes by ectomycorrhizal fungi (ECMF) under low potassium stress. The results showed that in normal potassium concentration, H⁺ effluxes by ectomycorrhizal fungi of the three isolates were not inhibited by trifluoperazine (TFP) and ethylene glycol tetraacetic acid (EGTA). In low potassium concentration, the H⁺ efflux by Bo08 and Sl01 was decreased significantly in the presence of TFP and EGTA. The results suggested that Ca²⁺would be a signal factor that could regulate H⁺ efflux by Bo08 and Sl01 in this experiment conditions of low potassium stress on the fungi.

Key words: potassium, Ca²⁺ signal inhibitors, ECMF

中图分类号:

S718.81

郭涛, 戴景程, 朱敏. 钾胁迫条件下Ca²⁺信号抑制剂对外生菌根真菌分泌H⁺的影响[J]. 林业科学, 2013, 49(12): 97-100.

Guo Tao, Dai Jingcheng, Zhu Min. Effects of Ca²⁺ Signal Inhibitors on H⁺ Effluxes by Ectomycorrhizal Fungi under Potassium Stress[J]. Scientia Silvae Sinicae, 2013, 49(12): 97-100.

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钾胁迫条件下Ca²⁺信号抑制剂对外生菌根真菌分泌H⁺的影响

Effects of Ca²⁺ Signal Inhibitors on H⁺ Effluxes by Ectomycorrhizal Fungi under Potassium Stress

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