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林业科学 ›› 2017, Vol. 53 ›› Issue (9): 81-89.doi: 10.11707/j.1001-7488.20170910

• 论文与研究报告 • 上一篇    下一篇

松树蜂入侵的混交林中针叶树种内生真菌多样性

王立祥, 刘晓博, 任利利, 石娟, 骆有庆   

  1. 北京林业大学林木有害生物防治北京市重点实验室 北京 100083
  • 收稿日期:2016-07-14 修回日期:2016-11-04 出版日期:2017-09-25 发布日期:2017-10-24
  • 基金资助:
    林业公益性行业科技专项(201404401);林业公益性行业科研专项(201504304)。

Variety of Endophytic Fungi Associated with Conifers in Mixed Conifer Forests Invaded by Sirex noctilio

Wang Lixiang, Liu Xiaobo, Ren Lili, Shi Juan, Luo Youqing   

  1. Beijing Key Laboratory for Forest Pest Control of Beijing Forestry University Beijing 100083
  • Received:2016-07-14 Revised:2016-11-04 Online:2017-09-25 Published:2017-10-24

摘要: [目的]研究混交林内4种针叶树内生真菌的种类、数量和分布,以及与受松树蜂危害的樟子松优势内生真菌的差异,从微观角度揭示不同树种内生真菌的多样性与松树蜂危害的关系。[方法]以松树蜂入侵后混交林中樟子松、兴安落叶松、红松、红皮云杉4种针叶树健康木(未受松树蜂危害)的内生真菌为研究对象,采用组织分离法、形态学和分子生物学相结合的鉴定方法。[结果]从4种针叶树树干内共分离得到内生真菌22属35种,其中:樟子松9属13种,红松7属13种,兴安落叶松8属17种,红皮云杉15属20种。4种针叶树内生真菌的数量:兴安落叶松 > 红皮云杉 > 红松 > 樟子松;内生真菌的种类:红皮云杉 > 兴安落叶松>红松 > 樟子松。主干上部真菌检出率最多,基部最少;菌株数上部最大,中部最小。4种针叶树内生真菌多样性水平存在差异,其中红皮云杉的多样性指数(H'=3.342 5)、均匀度指数(J=1.097 9)和丰富度指数(R=3.374 4)均最高。不同树种间内生真菌的相似性水平均较低,4种内生真菌在4种针叶树内均分离到,占4种针叶树内生真菌种类总数的11.43%;20种内生真菌在1种针叶树内能分离到,占4种针叶树内生真菌种类总数的57.14%,说明不同针叶树内生真菌对宿主的专一性较高。[结论]4种针叶树的木质部内都会存在一定数量真菌,且真菌种类差异较大。受松树蜂危害的樟子松优势内生真菌与4种针叶树健康木的优势内生真菌差别很大。相比其他树种,樟子松内生真菌的种类和数量均最少,可能有利于松树蜂共生菌Amylostereum areolatum的入侵,这也可能是松树蜂在混交林中只危害樟子松的原因。

关键词: 松树蜂, 内生真菌, 樟子松, 红松, 红皮云杉, 兴安落叶松, 多样性

Abstract: [Objective]The host trees are damaged by Sirex noctilio and Amylostereum areolatum coordinately. But the endophytic of the host trees also have an impact on the growth of A. areolatum. In China, the invasion of S. noctilio in coniferous mixed forest only results in the injury of Pinus sylvestris var. mongolica. This study was to clarify the differences in species, quantity and distribution of the conifer-inhabiting fungi associated with four kinds of conifers in coniferous mixed forest, and determine the dominant fungus of P. sylvestris var. mongolica invaded by S. noctilio, to reveal the relation between the endophytic diversity and S. noctilio invasion.[Method]We used tissue isolation, morphology and molecular biology methods to study the endophytic fungi of P. sylvestris var. mongolica,Larix gmelinii,Pinus koraiensis,Picea asperata in mixed conifer forest invaded by S. noctilio.[Result]Totally, we had isolated 35 species of endophytic fungi belonging to 22 genera from our experimental material. The endophytic fungi isolated from P. sylvestris var. mongolica,L. gmelinii,P. koraiensis and P. asperata refer to 13 species in 9 genera, 13 species in 7 genera, 17 species in 8 genera and 20 species in 15 genera, respectively. Comparing the results referred to endophytic fungi from the 4 kinds of conifers exhibited a conclusion of total amount:L. gmelinii > P. asperata > P. koraiensis > P. sylvestris var. mongolica; and the diversity of endophytic fungi:P. asperata > L. gmelinii > P. koraiensis > P. sylvestris var. mongolica. As the isolation ratio of sample from different trunk height of healthy pines, the maximum was from the upper part of trunk, whereas the minimum was from the basal part of trunk. The maximum strain species was from the upper part of trunk, and the minimum was from the middle part of trunk. The diversity level of endophytic fungi varies among the four kinds of conifers. The diversity index (H'=3.342 5), evenness index (J=1.097 9) and richness index (R=3.374 4) of P. asperata were the highest among the four species. The similarity of endophytic fungi were low among the four species. Four species of endophytic fungi can be isolated from all the four kinds of conifers, accounting 11.43% of the total species; 20 kinds of fungi were isolated in one kind of conifer, accounting 57.14% of the total species; which illustrates the host-specificity of the conifer-inhabiting fungi.[Conclusion]A certain amount of fungi are present in four kinds of conifers and species vary among this four conifers. Dominant fungi of P. sylvestris var. mongolica invaded by the S. noctilio are different from which of the other kinds of conifers in coniferous mixed forest. Both of the amount and kinds of fungi of P. sylvestris var. mongolica are the least compared to the other species. This may be beneficial for the invasion of A. areolatum and could be the potential causes of S. noctilio injury only happens to P. sylvestris var. mongolica.

Key words: Sirex noctilio, endophytic fungi community, P.sylvestris var.mongolica, Pinus koraiensis, Picea koraiensis, Larix gmelinii, diversity

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