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林业科学 ›› 2010, Vol. 46 ›› Issue (7): 84-91.doi: 10.11707/j.1001-7488.20100713

• 论文 • 上一篇    下一篇

月季切花品种间失水胁迫耐性与抗氧化酶和内肽酶之间的关联

谢利娟1,张华1,崔士超2   

  1. 1.深圳职业技术学院应用化学与生物技术学院 深圳 518055;2.云南农业大学园林园艺学院 昆明 650201
  • 收稿日期:2010-03-20 修回日期:2010-04-21 出版日期:2010-07-25 发布日期:2010-07-25

Involvement of Antioxidant Enzymes and Endoprotease in Tolerance to Water Deficit Stress in Cut Roses

Xie Lijuan1;Zhang Hua1;Cui Shichao2   

  1. 1.School of Applied Chemistry and Biotecnology,Shenzhen Polytechnic Shenzhen 518055;2.College of Horticulture and Landscape,Yunnan Agricultural University Kunming 650201
  • Received:2010-03-20 Revised:2010-04-21 Online:2010-07-25 Published:2010-07-25

摘要:

以切花月季品种‘Sunny leonidas’和‘Sun stone’为试材,探讨失水胁迫加快不同品种衰老进程与失水胁迫耐性差异、抗氧化酶和内肽酶的诱导种类以及活性的内在联系。结果表明: 24 h失水胁迫处理分别缩短‘Sunny leonidas’和‘Sun stone’瓶插寿命16.4%和36.9%,增加2者花瓣和叶片MDA含量,提高了SOD、CAT和POD酶活性,诱导并提高金属蛋白酶、巯基蛋白酶和丝氨酸蛋白酶3种内肽酶活性,叶片耐失水胁迫能力强于花朵。失水处理后的瓶插期间,‘Sunny leonidas’恢复能力强于‘Sun stone’,表现为前者花瓣和叶片的MDA含量低于后者,‘Sunny leonidas’花瓣中SOD,CAT和POD活性高峰(瓶插第4天)晚于‘Sun stone’(瓶插第3天),内肽酶活性在第5天显著增加,也晚于‘Sun stone’(瓶插第4天),2品种在失水胁迫后的瓶插期间诱导的种类主要为丝氨酸蛋白酶,‘Sunny leonidas’增加较为平缓,高峰出现在第5天,晚于‘Sun stone’。结果表明: ‘Sunny leonidas’较‘Sun stone’有较强的失水胁迫耐性可能与花瓣中SOD,CAT和POD活性高峰以及内肽酶活性的迅速增加相关联,2品种失水胁迫的差异与丝氨酸蛋白酶的活性相关联,SOD等抗氧化酶活性高峰和内肽酶活性显著升高与切花衰老有一定关联。

关键词: 月季, 切花, 水分胁迫, MDA, 抗氧化酶, 内肽酶

Abstract:

In this work, we investigated roles of endoproteases (EP) and main anti-oxidant enzymes in water deficit stress (WDS)-induced senescence in cut roses. Our results showed that 24 h WDS shortened vase life by 16.4% and 36.9% in ‘Sunny leonidas’ and ‘Sun stone’, respectively. WDS increased MDA content and the activity of anti-oxidant enzymes, including SOD (superoxide dismutase), catalase (CAT) and peroxidase (POD) in leaves and petals of both cultivars. WDS also enhanced activities of metallo proteinase, thiol proteinase and serine proteinase. When water available (WA), morphological appearance of ‘Sunny leonidas’ was restored better than ‘Sun stone’. Compared to the latter, ‘Sunny leonidas’ had lower MDA content of leaves and petals and delayed increase in activities of all three anti-oxidant enzymes. Serine proteinase was a major EP during WA in both cultivars, however its activity increased more rapidly and sharply in ‘Sun stone’ than in ‘Sunny leonidas’. It was suggested that anti-oxidant enzymes and EP were involved in the response of cut roses to WDS, and serine proteinase might be the major EP functioned in WDS.

Key words: rose, cut flower, water deficit stress, anti-oxidant enzymes, endopeptidase