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林业科学 ›› 2009, Vol. 12 ›› Issue (6): 162-165.doi: 10.11707/j.1001-7488.20090629

• 论文 • 上一篇    下一篇

荆条丛枝病植原体的分子检测及鉴定*

崔廷涛1 潘一展2 高 瑞1 邵云华1 李向东1时呈奎1 赵文军3 朱水芳3   

  1. (1.山东农业大学植物保护学院植物病理学系 泰安271018; 2.商丘职业技术学院 商丘476100; 3.中国检验检疫科学研究院动植物检疫研究所 北京100029)
  • 收稿日期:2008-05-12 修回日期:1900-01-01 出版日期:2009-06-25 发布日期:2009-06-25
  • 通讯作者: 李向东

Molecular Detection and Identification of the Phytoplasma Associated with Chaste Tree Witches’- Broom

Cui Tingtao1,Pan Yizhan2,Gao Rui1,Shao Yunhua1,Li Xiangdong1,Shi Chengkui1,Zhao Wenjun3,Zhu Shuifang3
  

  1. (1. Department of Plant Pathology, College of Plant Protection, Shandong Agricultural University Tai’an 271018; 2. Shangqiu Vocational and Technical College Shangqiu 476100; 3. Institute of Animal and Plant Quarantine, Chinese Academy of Inspection and Quarantine Beijing 100029)
  • Received:2008-05-12 Revised:1900-01-01 Online:2009-06-25 Published:2009-06-25

关键词: 荆条, 植原体, 丛枝病, 16S rRNA序列, 巢式PCR

Abstract:

Chaste tree (Vitex negundo var. heterophylla) witches’- broom was a new disease found in Mountain Taishan and the neighboring region. Phytoplasmal cells could be easily observed in the phloem sieve elements of diseased plants under electron microscope. The 16S rRNA gene of phytoplasma associated with this disease (CTWB) was amplified with nested-PCR with the universal primer pairs R16mF2/ R16mR1 and R16F2n/ R16R2 An amplified fragment of ca. 1.2 kb was sequenced and subjected to identity and phylogenetic analyses. Phytoplasma CTWB shared identities of more than 99.0% with members of Aster yellows group (16SrI) in 16S rRNA genes. It had the highest identity of 99.8% with phytoplasmas associated with Maize bushy stunt (subgroup 16SrI-B), and clustered into a common branch with phytoplasmas of the subgroup in the phylogenetic tree constructed with 16S rRNA genes. These results indicated that CTWB belonged to subgroup B of Candidatus Phytoplasma asteris (16SrI-B).

Key words: chaste tree, witches&rsquo, - broom, phytoplasma, 16S rRNA gene, nested-PCR