荆条丛枝病植原体的分子检测及鉴定*

doi:10.11707/j.1001-7488.20090629

林业科学 ›› 2009, Vol. 12 ›› Issue (6): 162-165.doi: 10.11707/j.1001-7488.20090629

荆条丛枝病植原体的分子检测及鉴定*

崔廷涛¹潘一展² 高瑞¹ 邵云华¹李向东¹时呈奎¹赵文军³ 朱水芳³

（1.山东农业大学植物保护学院植物病理学系泰安271018； 2.商丘职业技术学院商丘476100； 3.中国检验检疫科学研究院动植物检疫研究所北京100029）

收稿日期:2008-05-12 修回日期:1900-01-01 出版日期:2009-06-25 发布日期:2009-06-25
通讯作者: 李向东

Molecular Detection and Identification of the Phytoplasma Associated with Chaste Tree Witches’- Broom

Cui Tingtao¹,Pan Yizhan²,Gao Rui¹,Shao Yunhua¹,Li Xiangdong¹,Shi Chengkui¹,Zhao Wenjun³,Zhu Shuifang³

(1. Department of Plant Pathology, College of Plant Protection, Shandong Agricultural University Tai’an 271018; 2. Shangqiu Vocational and Technical College Shangqiu 476100; 3. Institute of Animal and Plant Quarantine, Chinese Academy of Inspection and Quarantine Beijing 100029)

Received:2008-05-12 Revised:1900-01-01 Online:2009-06-25 Published:2009-06-25

摘要/Abstract

关键词: 荆条, 植原体, 丛枝病, 16S rRNA序列, 巢式PCR

Abstract:

Chaste tree (Vitex negundo var. heterophylla) witches’- broom was a new disease found in Mountain Taishan and the neighboring region. Phytoplasmal cells could be easily observed in the phloem sieve elements of diseased plants under electron microscope. The 16S rRNA gene of phytoplasma associated with this disease (CTWB) was amplified with nested-PCR with the universal primer pairs R16mF2/ R16mR1 and R16F2n/ R16R2 An amplified fragment of ca. 1.2 kb was sequenced and subjected to identity and phylogenetic analyses. Phytoplasma CTWB shared identities of more than 99.0% with members of Aster yellows group (16SrI) in 16S rRNA genes. It had the highest identity of 99.8% with phytoplasmas associated with Maize bushy stunt (subgroup 16SrI-B), and clustered into a common branch with phytoplasmas of the subgroup in the phylogenetic tree constructed with 16S rRNA genes. These results indicated that CTWB belonged to subgroup B of Candidatus Phytoplasma asteris (16SrI-B).

Key words: chaste tree, witches&rsquo, - broom, phytoplasma, 16S rRNA gene, nested-PCR

崔廷涛潘一展高瑞邵云华李向东时呈奎赵文军朱水芳. 荆条丛枝病植原体的分子检测及鉴定*[J]. 林业科学, 2009, 12(6): 162-165.

Cui Tingtao;Pan Yizhan;Gao Rui;Shao Yunhua;Li Xiangdong;Shi Chengkui;Zhao Wenjun;Zhu Shuifang . Molecular Detection and Identification of the Phytoplasma Associated with Chaste Tree Witches’- Broom[J]. Scientia Silvae Sinicae, 2009, 12(6): 162-165.

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荆条丛枝病植原体的分子检测及鉴定*

Molecular Detection and Identification of the Phytoplasma Associated with Chaste Tree Witches’- Broom

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