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林业科学 ›› 2009, Vol. 12 ›› Issue (2): 147-151.doi: 10.11707/j.1001-7488.20090225

• 论文 • 上一篇    下一篇

泡桐丛枝植原体抗原膜蛋白基因序列分析与蛋白结构预测

岳红妮 吴云锋 史英姿   

  1. (西北农林科技大学植物保护学院 陕西省农业分子生物学重点实验室 杨凌712100)
  • 收稿日期:2008-01-06 修回日期:1900-01-01 出版日期:2009-02-25 发布日期:2009-02-25

Sequence Analysis and Structure Prediction of Antigenetic Membrane Protein Gene from Paulownia Witches' Broom Phytoplasma

Yue Hongni,Wu Yunfeng,Shi Yingzi   

  1. (Shaanxi Key Laboratory of Molecular Biology for Agriculture College of Plant Protection,Northwest A&F University Yangling 712100)
  • Received:2008-01-06 Revised:1900-01-01 Online:2009-02-25 Published:2009-02-25

关键词: 关键词:泡桐丛枝植原体, 抗原膜蛋白基因, 同源性, 蛋白结构

Abstract:

Abstract: Paulownia witches' broom disease which was caused by the paulownia witches' broom phytoplasma (PaWB) is one of the severest diseases in paulownia production. Antigenic membrane protein ( Amp ) gene was cloned from Paulownia plants infected with Paulownia witches' broom phytoplasma in Shaanxi Province. The gene was 696 bp in length, encoding a predicted protein of 231 amino acids. Homology analysis of sequence from PaWB and other 8 phytoplasmas of GenBank available showed that amp gene of PaWB was 100% identical to PaWB-Japan, and closely related to those of onion yellow ( AY ) and aster yellow ( OY ) in the same 16Sr Ⅰgroup with nucleotide acids sequences homology rate of 97%; However it had a lower nucleotide acids sequences homology rate of 37% with western X disease in 16Sr Ⅲ group. Prediction of protein structure showed that molecular weight of Amp was 24.7 ku and isoelectric point was 9.935. The Amp protein possessed acentral hydrophilic region and two hydrophobic transmembrane regions. There were several potential cleavage sites of signal peptide, the strongest cleavage site was at the end of Amp and there was no potential cleavage sites in the middle Amp. The information suggested that the protein should be of good antigenicity.

Key words: Key words: paulownia witches' broom phytoplasma, antigenic membrane protein, homology, structure of protein