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林业科学 ›› 2008, Vol. 44 ›› Issue (9): 152-156.doi: 10.11707/j.1001-7488.20080927

• 论文 • 上一篇    下一篇

土霉素对豫杂一号泡桐丛枝病幼苗形态和DNA甲基化水平的影响

黎明 翟晓巧 范国强 张变莉 刘飞   

  1. 河南农业大学泡桐研究所,郑州450002
  • 收稿日期:2006-09-29 修回日期:1900-01-01 出版日期:2008-09-25 发布日期:2008-09-25
  • 通讯作者: 范国强

Effect of Oxytetracycline on the Morphology of Seedling with Witches' Broom and DNA Methylation Level of Paulownia tomentosa×P. fortunei

Li Ming,Zhai Xiaoqiao,Fan Guoqiang,Zhang Bianli,Liu Fe   

  1. Institute of Paulownia,Henan Agricultutral University Zhengzhou 450002
  • Received:2006-09-29 Revised:1900-01-01 Online:2008-09-25 Published:2008-09-25

关键词: 豫杂一号泡桐, 丛枝病, 幼苗形态, 土霉素, DNA甲基化

Abstract: The effects of oxytetracycline on the morphology of the seedling with witches' broom and global DNA methylation level of Paulownia tomentosa × Paulownia fortunei were investigated.The results indicated that the infected seedlings by phytoplasma recovered to normal morphology of the healthy seedlings after being treated with more than 20 mg L-1 oxytetracycline,and the pathogen could not be detected with PCR in the seedlings.The global DNA methylation level of diseased seedlings was lower than that of healthy one.The oxytetracycline treatment was able to increase the DNA methylation level,and the methylation level of the diseased seedlings increased with the dose of applied oxytetracycline.However the increased methylation of infected seedlings with oxytetracycline treatment could still not reach to level of healthy seedling(CK),even the seedlings treated with lethal oxytetracycline concentration.

Key words: Paulownia tomentosa×P.fortunei, witches' broom plantlet morphology, oxytetracycline, DNA methylation