北京市区古枣树单株种源抗枣疯病测定与抗病品种(系)筛选

doi:10.11707/j.1001-7488.20180814

林业科学 ›› 2018, Vol. 54 ›› Issue (8): 124-132.doi: 10.11707/j.1001-7488.20180814

北京市区古枣树单株种源抗枣疯病测定与抗病品种(系)筛选

王合¹, 任争光², 潘彦平¹, 冯术快³, 林彩丽⁴, 常恩忠⁵, 于少帅⁴, 田国忠⁴

1. 北京市林业保护站北京 100029;
2. 北京农学院植物科学技术学院北京 102206;
3. 北京市昌平区林业植保站北京 102200;
4. 中国林业科学研究院森林生态环境与保护研究所国家林业局森林保护学重点实验室北京 100091;
5. 北京市怀柔区林木病虫防治检疫站北京 101400

收稿日期:2017-06-12 修回日期:2018-02-12 出版日期:2018-08-25 发布日期:2018-08-18
基金资助:
国家自然科学基金项目（31370644）；林业科学技术推广项目（枣疯病综合防治技术在北京地区试验推广）（京园绿推[2011]03号）。

Determination of Individual Jujube Trees against Jujube Witches'-Broom Disease and Screening of Resistant Varieties from the Ancient Individual Jujube Trees Growing in Beijing

Wang He¹, Ren Zhengguang², Pan Yanping¹, Feng Shukuai³, Lin Caili⁴, Chang Enzhong⁵, Yu Shaoshuai⁴, Tian Guozhong⁴

1. Beijing Forestry Protection Station Beijing 100029;
2. College of Plant Science and Technology, Beijing University of Agriculture Beijing 102206;
3. Forestry and Plant Protection Station of Changping District of Beijing Beijing 102200;
4. Key Laboratory of Forest Protection of State Forestry Administration Research Institute of Forest Ecology, Environment and Protection, CAF Beijing 100091;
5. Forestry Protection and Quarantine Station of Huairou District of Beijing Beijing 101400

Received:2017-06-12 Revised:2018-02-12 Online:2018-08-25 Published:2018-08-18

摘要/Abstract

摘要： [目的]调查北京枣树资源，了解城区古枣树对枣疯病的遗传抗性状况，为抗枣疯病品种的开发与利用提供基础数据。[方法]通过采集健康的古枣树单株接穗，利用嫁接传病的方法在北京疯枣园内开展抗枣疯病能力鉴定和抗病品系选育工作。[结果]自2012年始，在怀柔区经过对嫁接到发病砧木上的接穗的发病情况跟踪调查、数据统计分析和病原PCR检测，初步从147种接穗中筛选出具有一定抗病能力的接穗45种，其表现为嫁接接穗症状轻或无、病菌扩展与增殖慢、平均病情指数低等。2013年在昌平区的复筛试验中，进一步依据月度病情指数调查数据，从上述45种接穗中确定一批具有抗枣疯病能力稳定的品系。最后结合2014年的调查结果优先推选出7种正常生长结果、园艺学性状良好，具有利用价值前景的枣树品系。[结论]研究结果初步明确北京市古枣树中具有一定抗枣疯病能力的资源，筛选出具有一定抗病能力的古枣树株系，为今后抗枣疯病的枣树品种开发利用和病害防控提供重要资源和依据。

关键词: 古枣树, 枣疯病, 植原体, 抗病品种, 嫁接传病, 病原检测

Abstract: [Objective]This study aimed to understand the genetic resistance of jujube trees to jujube witches'-broom by investigating the resources of jujube trees in Beijing, which would provide basic data for the development and utilization of Jujube Witches' disease varieties.[Method]In this study, scions were collected from the healthy individual ancient jujube trees, and grafted onto the diseased jujube trees in a diseased jujube orchards to test the resistances to the jujube witches'-broom disease and screen the resistant varieties by the grafting inoculation method. [Result]As a result, 45 resistant scions with a certain resistance to the disease were screened out from 147 tested scions in Huairou district by tracking investigation since 2012, data statistical analysis, and PCR detection, and those resistant grafted scions displayed no or light symptoms, the spreading and propagation of phytoplasmas were slow, and the average disease index was low. A rescreening test was conducted in Changping district in 2013, a batch of strains with stable ability to resist jujube disease were determined from the above 45 scions according to the data of the monthly disease index survey. Combined with the survey results in 2014, 7 varieties derived from different scions were selected. Those varieties displayed normal growth and bearing fruits, and had good horticultural characteristics and potential utilization value. [Conclusion]This study has preliminarily clarified that the ancient jujube trees in Beijing have certain ability to resist Jujube Witches'-broom, and some disease-resistant cultivars have been screened, which lays a solid foundation for development and utilization of these resistant jujube varieties as well as for prevention and control of the disease.

Key words: ancient jujube, jujube witches'-broom disease, phytoplasma, resistant varieties, graft inoculation, pathogen detection

中图分类号:

S763.14

王合, 任争光, 潘彦平, 冯术快, 林彩丽, 常恩忠, 于少帅, 田国忠. 北京市区古枣树单株种源抗枣疯病测定与抗病品种(系)筛选[J]. 林业科学, 2018, 54(8): 124-132.

Wang He, Ren Zhengguang, Pan Yanping, Feng Shukuai, Lin Caili, Chang Enzhong, Yu Shaoshuai, Tian Guozhong. Determination of Individual Jujube Trees against Jujube Witches'-Broom Disease and Screening of Resistant Varieties from the Ancient Individual Jujube Trees Growing in Beijing[J]. Scientia Silvae Sinicae, 2018, 54(8): 124-132.

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北京市区古枣树单株种源抗枣疯病测定与抗病品种(系)筛选

Determination of Individual Jujube Trees against Jujube Witches'-Broom Disease and Screening of Resistant Varieties from the Ancient Individual Jujube Trees Growing in Beijing

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