关键词: 猪屎豆丛枝病, 植原体, 分子鉴定, 序列测定

Abstract:

Crotalaria witches’-broom phytoplasma (CrWB) collected from Hainan Province, was classified and identified by sequencing techniques and Virtual RFLP. Two different type universal primer pairs of phytoplasma were used to amplify a 1.8 kb fragment of 16S rDNA and a 1.3 kb fragment of rp gene. The PCRamplified products were cloned and sequenced. The results of sequencing and homologuous comparison with other phytoplasmas showed that Crotalaria witches’-broom phytoplasma shared 99.9 % similarity with peanut witches’broom phytoplasma in 16S rRNA gene and 99.9% in rp gene. Crotalaria witches'-broom phytoplasma is sorted into Peanut witches'-broom group(16SrII), 16SrIIA, according to the results of Virtual RFLP of 16S rDNA, phylogenetic analysis of 16S rRNA and rp gene, which is related to Ca. Phytoplasma australasiae.

Key words: Crotalaria witches&rsquo, broom phytoplasma, phytoplasma, molecular identification, sequencing