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林业科学 ›› 2006, Vol. 42 ›› Issue (4): 27-32.doi: 10.11707/j.1001-7488.20060405

• 论文及研究报告 • 上一篇    下一篇

夏季遮荫改善大田牡丹叶片光合功能的研究

郑国生1 何秀丽2,3   

  1. 1.莱阳农学院生命科学学院,青岛266109;2.中国农业大学农学与生物技术学院,北京100094;3.山东农业大学生命科学学院泰安271018
  • 收稿日期:2004-10-08 修回日期:1900-01-01 出版日期:2006-04-25 发布日期:2006-04-25

Studies on the Photosynthetic Improvement in the Leaves of Field Tree Peony through Shading Treatment in Summer

Zheng Guosheng1,He Xiuli2,3   

  1. 1. Department of Life Science, Laiyang Agricultural College Qingdao 266109; 2. College of Agronomy and Biotechnology, China Agricultural University Beijing 100094; 3. College of Life Science, Shandong Agricultural University Tai'an 271018
  • Received:2004-10-08 Revised:1900-01-01 Online:2006-04-25 Published:2006-04-25

摘要:

利用气体交换和叶绿素荧光分析技术,研究大田牡丹叶片在自然光照和夏季遮荫处理下光合作用日变化。结果表明:自然光下夏季牡丹叶片的净光合速率(Pn)明显低于春季,2个生长季节Pn日变化差异明显,即春季的Pn日变化呈“单峰型”曲线,夏季的Pn日变化为“双峰型”曲线,有明显的光合“午休”;羧化效率(CE)变化趋势与Pn相似;表观量子效率(AQY)中午降低,下午逐渐回升;光系统Ⅱ(PSⅡ)最大光化学效率Fv/Fm和最大荧光Fm日变化为倒“单峰型”曲线,中午明显降低;与夏季自然光下相比,夏季遮荫处理(遮光50%)的牡丹叶片Pn、AQY、CE升高;气孔限制值(Ls)和叶温(tl)降低;Fv/FmFm中午下降幅度显著减小。这些结果说明:夏季晴天中午高温、强光下,牡丹叶片PSⅡ反应中心发生了可逆失活,PSⅡ功能下调,光合作用的光抑制明显发生,遮荫可减轻光抑制,改善光合功能以增加光合产物积累。

关键词: 牡丹, 光合作用, 光抑制, 叶绿素荧光, 遮荫

Abstract:

Diurnal variation of photosynthesis under natural conditions and shading treatment in summer in the leaves of field Tree Peony(Paeonia suffruticosa) was studied using the technology of gas exchange and analyzing chlorophyll fluorescence. The results indicated that the curve of diurnal variation of net photosynthetic rate (Pn) was demonstrated single peak in spring,two peaks in clear days in summer,with midday depression of photosynthesis. The apparent quantum yield (AQY) and the carboxylation efficiency (CE) displayed significant midday decline. The maximal PSⅡ efficiency (Fv/Fm) and maximal fluorescence (Fm) demonstrated reverse single peaks, which decreased obviously in the midday. Through shading treatment in summer, the curve of diurnal variation of Pn was demonstrated single peak. Photosynthetic active radiation (PAR) and limit of stomatal (Ls) decreased, but stomatal conductance (Gs) and the internal leaf CO2 pressure (CI) increased compared with those in sun-light. And the decreased amplitude of Fv/Fm and Fm declined obviously. Shading treatment hardly affected the Fo. These results showed that PSⅡ reaction center exhibited a dynamic change on revisable inactivation; PSⅡ function exhibited obviously down-regulation and photoinhibition under strong midday light in summer. Shading treatment in summer can improve photosynthetic function and accelerate products by abating photoinhibition.

Key words: Tree Peony(Paeonia suffruticosa), photosynthesis, photoinhibition, chlorophyll fluorescence parameters, shading treatment