欢迎访问林业科学,今天是

林业科学 ›› 2006, Vol. 42 ›› Issue (4): 19-26.doi: 10.11707/j.1001-7488.20060404

• 论文及研究报告 • 上一篇    下一篇

白杨派杂种无性系及其亲本光合和生长对盐胁迫的反应

杨敏生1 李艳华2 梁海永1 王进茂1   

  1. 1.河北农业大学,保定071000;2.河北省环境科学研究院,石家庄050051
  • 收稿日期:2004-08-03 修回日期:1900-01-01 出版日期:2006-04-25 发布日期:2006-04-25

Comparison of Growth and Photosynthesis in Response to Salt Stress in Populus Hybridization Clones and Their Parent Species

Yang Minsheng1,Li Yanhua2,Liang Haiyong1,Wang Jinmao1   

  1. 1. Agricultural University of Hebei Baoding 071000; 2. Hebei Provincial Academy of Environmental Sciences Shijiazhuang 050051
  • Received:2004-08-03 Revised:1900-01-01 Online:2006-04-25 Published:2006-04-25

摘要:

以白杨派单交和双交杂种无性系1年生扦插苗为材料,分析不同盐胁迫条件下无性系的生长及光合系统的反应,并对无性系间的差异进行比较。研究表明:盐胁迫对叶片生长、苗高生长及苗木生物量积累均产生不同程度的影响。随着盐浓度升高,各项生长指标迅速下降,但下降的幅度不同,苗木生物量积累受影响最大。茎叶生长对盐胁迫的敏感性大于根系生长,从而导致根/(茎+叶)生物量随盐胁迫的发展而升高。盐胁迫下4个无性系的净光合速率(Pn)日变化基本上都呈单峰曲线,但随胁迫加强,Pn值迅速降低。盐胁迫下,叶位从幼到老,Pn表现为单峰型变化,中部功能叶Pn值最大,嫩叶和老叶较小,盐胁迫对无性系嫩叶Pn几乎无影响,却显著降低中部功能叶和老叶的Pn值,且随着胁迫时间延长,对叶片Pn的影响加强。盐胁迫降低各无性系的叶绿素含量,且使叶绿素荧光诱导动力学曲线发生明显变化,其参数Pn(荧光非光化学猝灭系数)值随盐浓度增加而上升,而qP(荧光光化学猝灭系数)、Fv/Fo(PSⅡ潜在活性)值随盐浓度增加而下降。盐胁迫对双交杂种无性系B430影响最小,单交无性系毛新杨和亲本新疆杨次之,亲本毛白杨对盐胁迫反应最敏感。

关键词: 白杨派杂种无性系, 盐胁迫, 生长, 净光合速率, 荧光动力学

Abstract:

The growth and photosynthetic system of the clones under different salt stress conditions and the differences among the clones were investigated using one-year-old seedlings of single poplar hybridization clones (Populus tomentosa×P.alba var. pyramidalis), double cross poplar hybridization clones [(P. tomentosa×P.alba var. pyramidalis)×(P. alba×P. tremula)] and their parent species (P. tomentosa and P.alba var. pyramidalis). The results indicated that there was genotypic difference in leaf growth, height and dry biomass in response to salt stress. With increasing salinity, the growth factors decreased rapidly, but differently in extent. The dry biomass was affected most under salt stress. The parts above ground of the plant were more susceptible to salt stress than that below the ground, leading to a higher root/stem ratio with increasing salt. The diurnal change of photosynthetic rates (Pn) in the four clones generally presented a single peak curve, but the Pn value decreased quickly with increasing salt stress. Under the salt stress, the Pn showed a single peak curve from the young leaves to the aged leaves. The Pn of young leaves was hardly influenced by salt stress but the Pn of older leaves decreased significantly. The influence of salt stress on Pn of leaves was even stronger with time. It was also found that the chlorophyll content of different clones declined and the curve of chlorophyll fluorescence induction kinetics changed obviously under the salt stress, and the parameter qN(coefficient of fluorescence non-photochemical quenching) increased, but qP (coefficient of fluorescence photochemical quenching) and Fv/Fo (PSⅡ photochemical activity) decreased with increasing salt stress. According to the above analysis, the double cross hybrid clones of B430 was resistant, P.alba var. pyramidalis and P. tomentosa×P.alba var. pyramidalis were moderate resistant, P. tomentosa was the most susceptible to salt stress.

Key words: poplar hybridization clones, salt stress, growth, photosynthetic rate, chlorophyll fluorescence induction kinetics