In this study, five typical vegetation types were selected along the elevational gradient in Shennongjia Nature Reserve, including shrubs, coniferous forest, mixed forest between coniferous and deciduous broad-leaved forest, deciduous broad-leaved forest, and evergreen broad-leaved forest. The content of soil organic carbon (SOC), water dissolved organic carbon (WDOC), labile organic carbon (LOC),and microbial biomass carbon (MBC) and their allocation proportions to SOC were analyzed. Furthermore, the Pearson relationship was analyzed between soil active organic carbon and plant diversity or soil physiochemical properties. The results showed that the soil organic carbon in the surface layer increased with the elevation increased, except for the deciduous broad-leaved forest. Distribution characteristics of MBC and LOC content along elevation gradient were obvious,but WDOC content was independent of the elevation gradient. The contents of the three kinds of active organic carbon in different elevational gradients were in the order of: LOC >MBC >WDOC. The allocation ratios of MBC, LOC and WDOC to SOC did not significantly alter with elevational gradient. Pearson relationship showed that SOC and MBC had a significant negative correlation with the diversity of trees and shrubs (P<0.05), while LOC and WDOC content had no significant correlation with plant diversity. In addition, SOC and MBC were significantly positively correlated with the content of soil moisture, total nitrogen, available nitrogen, total phosphorus and total sulfur (P<0.01). LOC had a significant and positive correlation with the content of soil moisture, total nitrogen, available nitrogen and total sulfur (P<0.01). There was a significant correlation between LOC and total phosphorus (P<0.05). The results revealed that these factors had a dominating impact on SOC, LOC and MBC. WDOC content had no obvious correlation with soil factors.