管状分子分化离体培养系统的建立及影响因素

doi:10.11707/j.1001-7488.20140821

Abstract

Abstract:

Tracheary elements (TEs) are main component in the xylem that are highly specialized for transporting water and solutes up the plant during plant growth and development, and have complex morphological characteristics. The mature plant cells can be converted into TEs by processes of differentiation or transdifferentiation in an appropriate vitro system. Recently constructed of vitro culture systems for Zinnia, Arabidopsis, Populus and other species have been facilitating our observation of the morphological changes during TEs differentiation and understanding the factors that influence the differentiation process. Previous researches indicated that TEs differentiation processes vary with plant cells, and many factors such as phytohormone, xylogen, Ca²⁺, osmotic pressure, initial cell population density, pH and so on, play great roles in regulating TEs differentiation. At present, the Zinnia vitro culture system is the relatively mature system for observing TEs differentiation while Populus system is the most promising system for woody plant. These model systems provide an excellent platform for study of TEs differentiation at single cell level, and greatly advance our understanding of TEs differentiation process and its control mechanism at the molecular level for ultimate goals of wood improvement.

Key words: tracheary element, in vitro culture system, cell differentiation, influencing factors

CLC Number:

S718.43

Du Yuliang, Liu Caihong, Chen Ye, Yin Zengfang. Establishment and Influencing Factors of in vitro Culture System for Tracheary Elements Differentiation[J]. Scientia Silvae Sinicae, 2014, 50(8): 146-153.

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Establishment and Influencing Factors of in vitro Culture System for Tracheary Elements Differentiation

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