基于RNA-Seq的油茶种子α-亚麻酸代谢途径及相关基因分析

doi:10.11707/j.1001-7488.20140810

Abstract

Abstract:

In an effort to better understand the factors that regulate α-linolenic acid metabolism in Camellia oleifera seeds, the transcriptome library and expression profile of C. oleifera seeds both in the fruit expanding period and at the peak stage of lipid biosynthesis have been constructed and the functional genes related to α-linolenic acid metabolism have been analyzed in detail. Study on transcriptome showed that there were one hundred and twelve non-redundant gene unigenes related to α-linolenic acid metabolism and ninety-four unigenes related to biosynthesis of unsaturated fatty acids of C. oleifera seeds, including twelve kinds of key enzyme genes regulating α-linolenic acid metabolism. The results of expression profile analysis showed that these genes had different expression patterns. These genes regulate the synthesis of α-linolenic acid and its conversion process to other unsaturated fatty acid at different developmental stage. The real-time quantitative RT-PCR analysis revealed relative expression regularity of the key enzyme genes such as LOX, AOS, ACOX, AOC, OPR, AACT, PLA2, HPL, DOX, MFP, FAD2, FAD3 in α-linolenic acid metabolism in fruit expanding period and the oil synthesis peak stage, which agreed with the results of expression profile analysis. The findings would provide a scientific basic for Camellia breeding of good quality with increasing content of α-linolenic acid.

Key words: Camellia oleifera, transcriptome, expression profile, α-linolenic acid metabolism, gene expression

CLC Number:

S718.46

Jiang Nan, Tan Xiaofeng, Zhang Lin, Zeng Yanling. Gene Analysis of α-Linolenic Acid Metabolism of Camellia oleifera Seeds Based on RNA-Seq[J]. Scientia Silvae Sinicae, 2014, 50(8): 68-75.

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Gene Analysis of α-Linolenic Acid Metabolism of Camellia oleifera Seeds Based on RNA-Seq

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