舞毒蛾LdOA1基因克隆分析及对3种杀虫剂胁迫的响应

doi:10.11707/j.1001-7488.20140815

Abstract

Abstract:

The G protein coupled receptors (GPCRs), known as transmembrane receptors, play an important role in drug research and development by activating G protein involved in inside signal transduction pathways. In present study, the full length cDNA of GPCR (namely LdOA1) was isolated from Lymantria dispar transcriptome. The open reading frame (ORF) of LdOA1 was 453 bp encoding a protein of 150 amino acid residues with the molecular mass of 17.54 kDa and theoretical pI of 9.76. Phylogenetic analysis of GPCR proteins showed LdOA1 of L. dispar clustered into a group with Danaus plexippus. We further investigated the expression of LdOA1 in 3^rd instar L. dispar larvae under deltamethrin, carbaryl and omethoate stresses using real-time PCR. The results showed that LdOA1 in L. dispar was obviously inhibited by deltamethrin, carbaryl and omethoate.

Key words: Lymantria dispar, LdOA1, insecticide, gene expression

CLC Number:

S718.7

Cao Chuanwang, Sun Lili, Wen Rongrong, Dou Xiaojie, Wang Zhiying. Cloning and Analysis of LdOA1in Lymantria dispar and Its Response to the Stress of Three Kinds of Insecticides[J]. Scientia Silvae Sinicae, 2014, 50(8): 102-107.

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Cloning and Analysis of LdOA1in Lymantria dispar and Its Response to the Stress of Three Kinds of Insecticides

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