杨树抗虫分子育种与转基因生物安全评价研究进展

doi:10.11707/j.1001-7488.LYKX20240263

Abstract

Abstract:

Populus spp., as an economic forest, ecological protection forest and national reserve forest construction species, plays a pivotal role as a significant carbon sink in achieving the goal of “carbon peaking and carbon neutrality” due to their fast-growing and wide-adaptable characteristics. However, the productivity of poplar plantations has been seriously affected by foliage-feeding and wood-boring insect pests. Therefore, it is an urgent task to elucidate the molecular mechanism of insect resistance in poplar and cultivate new insect-resistant and high-yielding poplar varieties for the development of poplar plantations. In this paper, we firstly outline the progress of molecular mechanism of poplars in reducing biotic stress through forming leaf trichomes, fortifying physical defenses including increased wood density, and rapid responses of secondary metabolites such as intrinsic phenolic glycosides and volatiles, as well as protease inhibitors. Then, we summarize the main applications of poplar cross-breeding, transgenic breeding technology, and multi-omics analysis in the cultivation of new varieties of poplars with insect-resistance and in the mining of key resistant sites and genes. The biosafety evaluation methods and progress of insect-resistant transgenic poplars are analyzed. Finally, it is proposed that in the future, emphasis should be placed on analyzing the molecular mechanism of poplar resistance to trunk borers, and integrating traditional cross-breeding approach with modern breeding techniques such as transgenics, gene editing and whole genome selection around the breeding goals to realize polymer breeding for insect resistance, enhanced quality, and increased yield. Concurrently, it is essential to establish and improve the biosafety assessment protocols and regulations for transgenic and gene-edited poplars for laying a solid theoretical and practical foundation for the genetic improvement and molecular design breeding of insect-resistant poplars.

Key words: poplar, foliage-feeding pests, trunk borers, biosafety assessment, biotechnology breeding

CLC Number:

S792.11

Lei Zhang,Xinglu Zhou,Lijuan Wang,Jianjun Hu. Advance of Poplar Molecular Breeding with Insect Resistance and Transgenic Biosafety Assessment Research[J]. Scientia Silvae Sinicae, 2025, 61(2): 190-203.

Figures/Tables 3

Fig.1

Table 1

Information table of insect resistant transgenic poplar"

基因 Genes	转化体 Variety	靶标害虫 Target pest	参考文献 Reference	行政许可阶段 Administrative licensing stage
BtCry1Ac	欧洲黑杨 P. nigra	杨尺蠖、舞毒蛾 A. cinerarius, L. dispar	田颖川等, 1993	安全证书Safety certificate
BtCry1A	健杨94 P. × euramericana cv. ‘Robusta 94’	舞毒蛾、膜肩网蝽 L. dispar, Hegesidemus habrus	李淑梅等, 2008	环境释放和生产性试验Environmental release and production test
BtCry1A	中嘉8号 P. deltoides (I-63× I-69)	杨扇舟蛾 C. anachoreta	康薇等, 2009
BtCry1A	山新杨 P. davidiana × P. bolleana	美国白蛾、舞毒蛾 H. cunea, L. dispar	Wu et al., 2019b
BtCry1Ah	山新杨 P. davidiana × P. bolleana	美国白蛾、舞毒蛾 H. cunea, L. dispar	Ding et al., 2017
BtCry1Ah	南林895 P. deltoides × P. euramericana ‘Nanlin 895’	美国白蛾 H. cunea	Xu et al., 2019	中间试验Intermediate Experiment
BtCry3A	741杨 P. alba × (P. davidiana + P. simonii) × P. tomentosa	桑天牛 A. germari	甄志先等, 2007	环境释放和生产性试验Environmental release and production test
BtCry3A	84K杨 P. alba × P. glandulosa ‘84K’	光肩星天牛 A. glabripennis	张冰玉等, 2006
BtCry3Bb	山新杨 P. davidiana × P. bolleana	柳蓝叶甲 Plagiodera versicolora	Xu et al., 2020
BtCry9Aa3	山新杨 P. davidiana × P. bolleana	美国白蛾、舞毒蛾 H. cunea, L. dispar	Ding et al., 2017
PeWRKY31	烟草 Nicotiana tabacum	美国白蛾 H. cunea	Yu et al., 2021
PeWRKY41	84K杨 P. alba × P. glandulosa ‘84K’	美国白蛾 H. cunea	Yu et al., 2022
Cpti	毛白杨 P. tomentosa	美国白蛾 H. cunea	郝贵霞等, 2000
Cpti	新疆杨 P. alba var. pyramidalis	杨尺蠖 A. cinerarius	诸葛强等, 2003
Cpti	欧美杨107 P. × euramericana ‘Neva’	扁刺蛾 Thosea sinensis	赵强等, 2005
AaIT	小叶杨×美洲黑杨N106 P. deltoides × P. simonii	舞毒蛾 L. dispar	伍宁丰等, 2000
BtCry1Ac + API	741杨 P. alba × (P. davidiana + P. simonii) × P. tomentosa	杨扇舟蛾 C. anachoreta	郑均宝等, 2000	安全证书Safety certificate
BtCry1Ac + API	三倍体毛白杨 (P. tomentosa × P. bolleana) × P. tomentosa	舞毒蛾、杨扇舟蛾 L. dispar, C. anachoreta	杨敏生等, 2006	中间试验Intermediate experiment
BtCry1Ac + API	84K杨 P. alba × P. glandulosa ‘84K’	杨扇舟蛾 C. anachoreta	李科友等, 2007
BtCry1Ac + API	欧美杨107 P. euramericana ‘Neva’	美国白蛾 H. cunea	杨艳丽等, 2012
BtCry1Ac + Cpti	美洲黑杨小叶杨杂种NL-80106 P. deltoides × P. simonii ‘NL-80106’	舞毒蛾 L. dispar	饶红宇等, 2000
BtCry1Ac + BtCry3A	比利尼杨 P. × euramericana cv. 'Bellini'	柳蓝叶甲 P. versicolora	Dong et al., 2015	环境释放和生产性试验Environmental release and production test
Bt + Cpti	南林895 P. deltoides × P. euramericana ‘Nanlin 895’	杨小舟蛾 M. troglodyta	诸葛强等, 2006	中间试验Intermediate experiment
BtCry3A + Oc-i	84K杨 P. alba × P. glandulosa ‘84K’	光肩星天牛 A. glabripennis	张冰玉等, 2005
BtCry3A + BtCry1Ac	欧美杨107 P. × euramericana ‘Neva’	光肩星天牛 A. glabripennis	Ren et al., 2021	环境释放和生产性试验Environmental release and production test
BtCry3A + PI	欧洲黑杨 P. nigra	舞毒蛾 L. dispar	李明亮等, 2000
BtCry3Aa-P2	窄冠黑杨 P. deltoides ‘Lux’ × (P. deltoides ‘Shanhaiguan’ × P. simonii) ‘Zhaiguan’		山东农业大学	中间试验Intermediate experiment
BtCry3Aa-P2	窄冠黑杨 P. deltoides ‘Lux’ × (P. deltoides ‘Shanhaiguan’ × P. simonii) ‘Zhaiguan’		中国林业科学研究院	中间试验Intermediate experiment
Bt-C肽+蜘蛛杀虫肽	小黑杨 P. × Xiaohei	舞毒蛾、杨小舟蛾、杨扇舟蛾和分月扇舟蛾 L. dispar, M. troglodyte, C. anachoreta, Clostera anastomosis	常玉广等, 2004
Bt+蜘蛛杀虫肽	山新杨 P. davidiana × P. bolleana		左丽丽等, 2009
Bt+蜘蛛杀虫肽	欧美杨108 P × euramericana ‘Guariento’	舞毒蛾 L. dispar	遇文婧等, 2009
Chitinase-BmkIT	青杨 P. cathayana	美国白蛾 H. cunea	杨利艳等, 2008
BtCry1Ac + BtCry3A + API	741杨 P. alba × (P. davidiana + P. simonii) × P. tomentosa	柳蓝叶甲、美国白蛾 P. versicolora, H. cunea	王桂英等, 2012	中间试验Intermediate experiment
BtCry1Ac + BtCry3A + BADH	欧美杨107 P. × euramericana ‘Neva’	斜纹夜蛾 Spodoptera litura	任亚超, 2013	环境释放和生产性试验Environmental release and production test
BtCry1Ac + BtCry3A + NTHK1	欧美杨107 P. × euramericana ‘Neva’	斜纹夜蛾 S. litura	张旭, 2013	环境释放和生产性试验Environmental release and production test
BtCry3A + BtCry1Ac + mtlD + BADH	欧洲黑杨 P. nigra	美国白蛾、斜纹夜蛾 H. cunea, S. litura	Zhou et al., 2020b	中间试验Intermediate experiment

Table 1

Fig.2

References 0

	曹庆杰, 迟德富, 宇　佳, 等. 10~11年生杨树品系抗杨干象水平及其与树干物理特性的关系. 林业科学研究, 2016, 29 (2): 183- 190.
	Cao Q J, Chi D F, Yu J, et al. Relationship between resistance level of 10- and 11-year-old poplar strains to Cryptorrhynchus lapathi L. (Coleoptera: Curculionidae) and the physical properties of poplar trunk. Forest Research, 2016, 29 (2): 183- 190.
	常玉广, 刘桂丰, 姜　静, 等. 小黑杨抗虫基因的遗传转化. 东北林业大学学报, 2004, 32 (6): 30- 31.
	Chang Y G, Liu G F, Jiang J, et al. The genetic transformation of the genes resistant to insect for Populus xiaohei. Journal of Northeast Forestry University, 2004, 32 (6): 30- 31.
	陈盼飞, 任亚超, 张　军, 等. 8年生嫁接转基因杨树Bt毒蛋白的表达与运输. 林业科学, 2016, 52 (7): 46- 52.
	Chen P F, Ren Y C, Zhang J, et al. Expression and transportation of Bt toxic protein in 8-year-old grafted transgenic poplar. Scientia Silvae Sinicae, 2016, 52 (7): 46- 52.
	国家林业和草原局. 2023. 中国林业和草原统计年鉴. 北京: 中国林业出版社, 106–107.
	National Forestry and Grassland Administration. 2023. China forestry and grassland statistical yearbook. Beijing: China Forestry Publishing House, 106–107. ［in Chinese］
	郝贵霞, 朱　祯, 朱之悌. 转CpTI基因毛白杨的获得. 林业科学, 2000, 36 (S1): 116- 119.
	Hao G X, Zhu Z, Zhu Z D. Obtaining of cowpea proteinase inhibitor transgenic Populus tomentosa. Scientia Silvae Sinicae, 2000, 36 (S1): 116- 119.
	侯荣轩, 赵　烨, 田彦挺, 等. 实验田4年生转基因毛白杨嫁接苗和自根苗安全性研究. 林业科学研究, 2022, 35 (2): 56- 66.
	Hou R X, Zhao Y, Tian Y T, et al. Study on safety of grafted and self-rooted seedlings of 4-year-old transgenic Populus tomentosa in trial plots. Forest Research, 2022, 35 (2): 56- 66.
	胡建军, 杨敏生, 卢孟柱. 我国抗虫转基因杨树生态安全性研究进展. 生物多样性, 2010, 18 (4): 336- 345. doi: 10.3724/SP.J.1003.2010.336
	Hu J J, Yang M S, Lu M Z. Advances in biosafety studies on transgenic insect-resistant poplars in China. Biodiversity Science, 2010, 18 (4): 336- 345. doi: 10.3724/SP.J.1003.2010.336
	胡建军, 赵自成, 苏雪辉, 等. 杨树新品种‘中成1号’. 林业科学, 2014a, 50 (5): 159.
	Hu J J, Zhao Z C, Su X H, et al. A new poplar variety of Populus deltoides ‘Zhongcheng 1’. Scientia Silvae Sinicae, 2014a, 50 (5): 159.
	胡建军, 赵自成, 苏雪辉, 等. 杨树新品种‘中豫1号’. 林业科学, 2014b, 50 (7): 170.
	Hu J J, Zhao Z C, Su X H, et al. A new poplar variety of Populus deltoides ‘Zhongyu 1’. Scientia Silvae Sinicae, 2014b, 50 (7): 170.
	贾会霞, 孙　佩, 李建波, 等. 丹红杨×转BtCry1Ac欧洲黑杨杂交子代抗虫性及生长量测定. 分子植物育种, 2017, 15 (10): 4101- 4109.
	Jia H X, Sun P, Li J B, et al. Insect-resistance and growth measurement of hybrid progeny from Populus deltoides cl. ‘Danhong’ and transgenic P. nigra with BtCry1Ac gene. Molecular Plant Breeding, 2017, 15 (10): 4101- 4109.
	康　薇, 郑　进, 李　兵, 等. 转Bt基因杨树对杨扇舟蛾的抗性. 中国生物防治, 2009, 25 (2): 125- 128.
	Kang W, Zheng J, Li B, et al. Resistance of transgenic Bt poplar to Clostera anachorta. Chinese Journal of Biological Control, 2009, 25 (2): 125- 128.
	李会平, 黄大庄, 王志刚, 等. 杨树形态特征、组织结构与光肩星天牛危害的关系. 东北林业大学学报, 2004, 32 (6): 111- 112.
	Li H P, Huang D Z, Wang Z G, et al. Relationships between morphological characteristics and tissue structure of poplars and damage by Anophora glabripennis Motsch. Journal of Northeast Forestry University, 2004, 32 (6): 111- 112.
	李会平, 王志刚, 杨敏生, 等. 杨树单宁与酚类物质种类及含量与光肩星天牛危害之间关系的研究. 河北农业大学学报, 2003, 26 (1): 36- 39.
	Li H P, Wang Z G, Yang M S, et al. The relation between tannin and phenol constituents and resistance to Anoplophora glabripennis of various poplar tree species. Journal of Hebei Agricultural University, 2003, 26 (1): 36- 39.
	李科友, 樊军锋, 赵　忠, 等. 银腺杨转CryIAc和API双价抗虫基因的研究. 林业科学研究, 2007, 20 (5): 699- 704.
	Li K Y, Fan J F, Zhao Z, et al. Transformation of CryIAc and API two insect-resistant genes to poplar (Populus alba × P. glandulosa). Forest Research, 2007, 20 (5): 699- 704.
	李明亮, 张　辉, 胡建军, 等. 转Bt基因和蛋白酶抑制剂基因杨树抗虫性的研究. 林业科学, 2000, 36 (2): 93- 97.
	Li M L, Zhang H, Hu J J, et al. Study on insect-resistant transgenic poplar plants containing both Bt and PI gene. Scientia Silvae Sinicae, 2000, 36 (2): 93- 97.
	李淑梅, 张春玲, 胡建军, 等. 转基因抗虫杨树新品种‘健杨94’. 林业科学, 2008, 44 (7): 141.
	Li S M, Zhang C L, Hu J J, et al. A new insect resistant transgenic poplar variety Populus × euramericana cv. ‘Robusta 94'. Scientia Silvae Sinicae, 2008, 44 (7): 141.
	母连胜, 何　勇, 罗　岸, 等. 质体基因工程在植物育种中的应用研究进展. 河南农业科学, 2017, 46 (6): 1- 12.
	Mu L S, He Y, Luo A, et al. Progress on application of plastid genetic engineering in plant breeding. Journal of Henan Agricultural Sciences, 2017, 46 (6): 1- 12.
	饶红宇, 陈　英, 黄敏仁, 等. 杨树NL-80106转Bt基因植株的获得及抗虫性. 植物资源与环境学报, 2000, 9 (2): 1- 5.
	Rao H Y, Chen Y, Huang M R, et al. Genetic transformation of poplar NL-80106 transferred by Bt gene and its insect-resistance. Journal of Plant Resources and Environment, 2000, 9 (2): 1- 5.
	任敏霞, 李　探, 张子恒, 等. 转BtCry1Ac与API基因107杨对节肢动物群落多样性及稳定性的影响. 林业科学, 2022, 58 (4): 110- 118.
	Ren M X, Li T, Zhang Z H, et al. Effects of transgenic BtCry1Ac and APl gene in poplar 107 on diversity and stability of arthropod community. Scientia Silvae Sinicae, 2022, 58 (4): 110- 118.
	任亚超. 2013. 多基因植物转化载体对烟草和欧美杨107杨的遗传转化. 保定: 河北农业大学.
	Ren Y C. 2013. Genetic transformation of tobacco and Populus × euramericana ‘Neva’ by multi-gene plant transformation vector. Baoding: Hebei Agricultural University. ［in Chinese］
	沈文静, 刘来盘, 方志翔, 等. 转基因杨树林下种植转基因棉花对转基因杨棉复合系统内节肢动物群落多样性的影响. 昆虫学报, 2021, 64 (10): 1187- 1195.
	Shen W J, Liu L P, Fang Z X, et al. Effects of planting transgenic cotton beneath transgenic poplar trees on the diversity of arthropod community in the transgenic poplar-cotton composite system. Acta Entomologica Sinica, 2021, 64 (10): 1187- 1195.
	田颖川, 李太元, 莽克强, 等. 抗虫转基因欧洲黑杨的培育. 生物工程学报, 1993, 9 (4): 291- 297, 395.
	Tian Y C, Li T Y, Mang K Q, et al. Insect tolerance of transgenic Populus nigra plants transformed with Bacillus thuringiensis toxin gene. Chinese Journal of Biotechnology, 1993, 9 (4): 291- 297, 395.
	王桂英, 杨敏生, 霍雪梅, 等. 741杨双Bt基因的遗传转化及转基因株系的抗虫性. 林业科学, 2012, 48 (9): 42- 49.
	Wang G Y, Yang M S, Huo X M, et al. Transformation of 741 poplar with double Bt genes and the insect-resistance of the transgenic plant. Scientia Silvae Sinicae, 2012, 48 (9): 42- 49.
	王连荣, 薛拥志, 张德健, 等. 2021. 转基因杨树嫁接苗中外源BtCry3A基因的表达及其产物运输. 分子植物育种, 19(9): 2906–2911.
	Wang L R, Xue Y Z, Zhang D J, et al . 2020. Expression and products transportation of BtCry3A gene in the grafted seedlings of transgenic poplar. Molecular Plant Breeding, 19(9): 2906–2911. ［in Chinese］
	王　璞, 郭同斌, 魏　辉, 等. 转Bt基因‘南林895’杨对美国白蛾和杨小舟蛾抗虫性分析. 分子植物育种, 2020, 18 (14): 4645- 4656.
	Wang P, Guo T B, Wei H, et al. Analysis of insect resistance of Bt-transgenic poplar (NL-895) to Hyphantria cunea and Micromelalopha troglodyte. Molecular Plant Breeding, 2020, 18 (14): 4645- 4656.
	王欣玉, 刘勇波. 2020. 转基因植物与近缘种之间基因流的研究进展. 应用生态学报, 31(9): 3207–3215.
	Wang X Y, Liu Y B. 2020. Research advances in gene flow between transgenic plants and their relatives. Chinese Journal of Applied Ecology, 31(9): 3207–3215. [in Chinese]
	王云霖. 我国人工林发展研究. 林业资源管理, 2019, 2 (1): 6- 11.
	Wang Y L. Review on China’s plantation development since the reform and opening up. Forest and Grassland Resources Research, 2019, 2 (1): 6- 11.
	伍宁丰, 孙　芹, 姚　斌, 等. 抗虫的转AaIT基因杨树的获得. 生物工程学报, 2000, 16 (2): 13- 17.
	Wu N F, Sun Q, Yao B, et al. Insect resistant transgenic poplar expressing AalT gene. Chinese Journal of Biotechnology, 2000, 16 (2): 13- 17.
	谢　兴. 2010. 杨树枝条酚酸的提取与分析及其对青杨天牛的影响. 哈尔滨: 东北林业大学.
	Xie X. 2010. Extraction and analysis of phenolic acids in poplar branches and their effect to Saperda populnea L. Harbin: Northeast Forestry University. ［in Chinese］
	杨利艳, 孙　毅, 谢莉琴. 转新型双价抗虫基因杨树对光肩星天牛及美国白蛾的抗性鉴定. 昆虫学报, 2008, 51 (8): 844- 848.
	Yang L Y, Sun Y, Xie L Q. Bioassays of resistance of transgenic poplar with novel binary insect-resistant genes to Anoplophora glabripennis (Coleoptera: Cerambycidae) and Hyphantria cunea (Lepidoptera: Arctiidae). Acta Entomologica Sinica, 2008, 51 (8): 844- 848.
	杨敏生, 李志兰, 王　颖, 等. 双抗虫基因对三倍体毛白杨的转化和抗虫性表达. 林业科学, 2006, 42 (9): 61- 68.
	Yang M S, Li Z L, Wang Y, et al. Transformation and expression of two insect-resistant genes to hybrid triploid of Chinese white poplar. Scientia Silvae Sinicae, 2006, 42 (9): 61- 68.
	杨艳丽, 刘兴菊, 刘桂林, 等. 双抗虫基因转化欧美杨107的研究. 北方园艺, 2012, (7): 120- 122.
	Yang Y L, Liu X J, Liu G L, et al. Study on two insect-resistant gene transformation of poplar 107. Northern Horticulture, 2012, (7): 120- 122.
	遇文婧, 邹传山, 王志英, 等. 欧美杨108号转蜘蛛杀虫肽与Bt毒蛋白嵌合基因的研究. 植物研究, 2009, 29 (5): 603- 606.
	Yu W J, Zou C S, Wang Z Y, et al. Populus nigra × Populus deltoids "108" transformed the chimeric genes of spider insecticidal and Bt. Bulletin of Botanical Research, 2009, 29 (5): 603- 606.
	张冰玉, 苏晓华, 李义良, 等. 转双价抗蛀干害虫基因杨树的获得及其抗虫性鉴定. 林业科学研究, 2005, 18 (3): 364- 368.
	(Zhang B Y, Su X H, Li Y L, et al. Transformation of poplar (Populus alba × P. glandulosa cv. ‘84K’) with binary insect resistant genes and analysis of insect resistance. Forest Research, 2005, 18 (3): 364- 368.
	张冰玉, 苏晓华, 李义良, 等. 转抗鞘翅目害虫基因银腺杨的获得及其抗虫性的初步研究. 北京林业大学学报, 2006, 28 (2): 102- 105.
	Zhang B Y, Su X H, Li Y L, et al. Production of Populus alba × P. glandulosa with a coleopterous insect resistant gene and analysis of insect resistance. Journal of Beijing Forestry University, 2006, 28 (2): 102- 105.
	张　阔, 魏建荣, 李　臻, 等. 杨树受机械损伤与光肩星天牛危害的防御性反应. 应用生态学报, 2021, 32 (11): 4139- 4146.
	Zhang K, Wei J R, Li Z, et al. Defensive responses of Populus deltoides cl. Beikang to mechanical injury and Anoplophora glabripennis infection. Chinese Journal of Applied Ecology, 2021, 32 (11): 4139- 4146.
	张　磊, 胡建军. 转BtCry1Ac欧洲黑杨的外源基因插入位点分析及特异性检测. 林业科学, 2020, 56 (10): 45- 52.
	Zhang L, Hu J J. An analysis of T-DNA insertion loci and detection of the locus-specific of transgenic Populus nigra lines with BtCry1Ac. Scientia Silvae Sinicae, 2020, 56 (10): 45- 52.
	张　娜, 刘志伟, 刘德广. 植物蛋白酶抑制剂的抗虫性研究进展. 植物保护, 2022, 48 (6): 238- 247, 277.
	Zhang N, Liu Z W, Liu D G. Advances in plant protease inhibitors against insects. Plant Protection, 2022, 48 (6): 238- 247, 277.
	张　旭. 2013. 抗虫耐盐多基因对烟草和107杨的遗传转化及表达研究. 保定: 河北农业大学.
	Zhang X. 2013. Genetic transformation of the insect-resistant and salt-tolerant polygenes in tobacco and Populus × euramericana ‘Neva’ and the expression analysis. Baoding: Hebei Agricultural University. ［in Chinese］
	赵　强, 赵志文, 张廷婷, 等. 豇豆胰蛋白酶抑制剂(CpTI)基因转化欧美杨的研究. 生物技术通报, 2005, (4): 54- 58.
	Zhao Q, Zhao Z W, Zhang T T, et al. Transformation of Populus euramericana with CpTI gene. Biotechnology Bulletin, 2005, (4): 54- 58.
	甄志先, 李　静, 梁海永, 等. 转BtCry3A基因杨树毒蛋白表达及对桑天牛抗性的研究. 蚕业科学, 2007, 33 (4): 538- 542. doi: 10.3969/j.issn.0257-4799.2007.04.004
	Zhen Z X, Li J, Liang H Y, et al. Expressions of BtCry3A gene in transgenic polar and its resistance against Apriona germari. Acta Sericologica Sinica, 2007, 33 (4): 538- 542. doi: 10.3969/j.issn.0257-4799.2007.04.004
	郑均宝, 梁海永, 高宝嘉, 等. 转双抗虫基因741毛白杨的选择及抗虫性. 林业科学, 2000, 36 (2): 13- 19,129.
	Zheng J B, Liang H Y, Gao B J, et al. Selection and insect resistance of transgenic hybrid poplar 741 carrying two insect/resistant genes. Scientia Silvae Sinicae, 2000, 36 (2): 13- 19,129.
	周培军, 李玲玲, 李红岩, 等. 转Bt基因‘南林895’杨时空表达及生物安全分析. 分子植物育种, 2022, 20 (5): 1568- 1580.
	Zhou P J, Li L L, Li H Y, et al. Spatio-temporal expression and biosafety analysis of transgenic 'Nanlin-895' poplar with Bt. Molecular Plant Breeding, 2022, 20 (5): 1568- 1580.
	诸葛强, 房　丹, 李秀芬, 等. 美洲黑杨杂种优良无性系转抗虫基因(Bt和CpTⅠ)的研究. 分子植物育种, 2006, 4 (6): 819- 824.
	Zhuge Q, Fang D, Li X F, et al. Transformation of Populus × euramericana cv. ‘Nanlin895’using Bt and CpTI insect-resistant genes. Molecular Plant Breeding, 2006, 4 (6): 819- 824.
	诸葛强, 王婕琛, 陈　英, 等. 豇豆胰蛋白酶抑制剂(CpTI)抗虫转基因新疆杨的获得. 分子植物育种, 2003, 1 (4): 491- 496.
	Zhuge Q, Wang J C, Chen Y, et al. Transgenic plantlets of Populus alba var. pyramidalis by transformation with CpTl gene. Molecular Plant Breeding, 2003, 1 (4): 491- 496.
	左丽丽, 王志英, 梁　臣, 等. 山新杨转Bt+蜘蛛杀虫肽基因的分析. 东北林业大学学报, 2009, 37 (7): 112- 114.
	Zuo L L, Wang Z Y, Liang C, et al. Transformation of Populus davidiana × P. bollena with Bt+spider toxin gene. Journal of Northeast Forestry University, 2009, 37 (7): 112- 114.
	Barker H L, Riehl J F, Bernhardsson C, et al. Linking plant genes to insect communities: identifying the genetic bases of plant traits and community composition. Molecular Ecology, 2019, 28 (19): 4404- 4421. doi: 10.1111/mec.15158
	Barrios-San Martín J, Quiroz A, Verdugo J A, et al. Host selection and probing behavior of the poplar aphid Chaitophorus leucomelas (Sternorrhyncha: Aphididae) on two poplar hybrids with contrasting susceptibility to aphids. Journal of Economic Entomology, 2014, 107 (1): 268- 276. doi: 10.1603/EC13282
	Beaulieu J, Nadeau S, Ding C, et al. Genomic selection for resistance to spruce budworm in white spruce and relationships with growth and wood quality traits. Evolutionary Applications, 2020, 13 (10): 2704- 2722. doi: 10.1111/eva.13076
	Bewg W P, Harding S A, Engle N L, et al. Multiplex knockout of trichome-regulating MYB duplicates in hybrid poplar using a single gRNA. Plant Physiology, 2022, 189 (2): 516- 526. doi: 10.1093/plphys/kiac128
	Boeckler G A, Gershenzon J, Unsicker S B 2011. Phenolic glycosides of the Salicaceae and their role as anti-herbivore defenses. Phytochemistry, 72(13): 1497–1509.
	Caccamo M. New precision-breeding law unlocks gene editing in England. Nature Biotechnology, 2023, 41 (6): 752- 753. doi: 10.1038/s41587-023-01795-8
	Carletti G, Carra A, Allegro G, et al. QTLs for woolly poplar aphid (Phloeomyzus passerinii L.) resistance detected in an inter-specific Populus deltoides × P. nigra mapping population. PLoS ONE, 2016, 11 (3): e0152569. doi: 10.1371/journal.pone.0152569
	Chen Q, Liang X, Wu C L, et al. Overexpression of leucoanthocyanidin reductase or anthocyanidin reductase elevates tannins content and confers cassava resistance to two-spotted spider mite. Frontiers in Plant Science, 2022, 13, 994866. doi: 10.3389/fpls.2022.994866
	Chen X H, Dong Y, Huang Y L, et al. Whole-genome resequencing using next-generation and Nanopore sequencing for molecular characterization of T-DNA integration in transgenic poplar 741. BMC Genomics, 2021, 22 (1): 329. doi: 10.1186/s12864-021-07625-y
	Chen Y, Su D, Li J, et al. Overexpression of bHLH95, a basic helix-loop-helix transcription factor family member, impacts trichome formation via regulating gibberellin biosynthesis in tomato. Journal of Experimental Botany, 2020, 71 (12): 3450- 3462. doi: 10.1093/jxb/eraa114
	DeWoody J, Viger M, Lakatos F, et al. Insight into the genetic components of community genetics: QTL mapping of insect association in a fast-growing forest tree. PLoS ONE, 2013, 8 (11): e79925. doi: 10.1371/journal.pone.0079925
	Ding L P, Chen Y J, Wei X L, et al. Laboratory evaluation of transgenic Populus davidiana × Populus bolleana expressing Cry1Ac + SCK, Cry1Ah3, and Cry9Aa3 genes against Gypsy moth and fall webworm. PLoS ONE, 2017, 12 (6): e0178754. doi: 10.1371/journal.pone.0178754
	Dong Y, Du S S, Zhang J, et al. Differential expression of dual Bt genes in transgene poplar Juba (Populus deltoides cv. 'Juba') transformed by two different transformation vectors. Canadian Journal of Forest Research, 2015, 45 (1): 60- 67. doi: 10.1139/cjfr-2014-0335
	Eberl F, Fabisch T, Luck K, et al. Poplar protease inhibitor expression differs in an herbivore specific manner. BMC Plant Biology, 2021, 21 (1): 170. doi: 10.1186/s12870-021-02936-4
	Eberl F, Hammerbacher A, Gershenzon J, et al. Leaf rust infection reduces herbivore-induced volatile emission in black poplar and attracts a generalist herbivore. New Phytologist, 2018, 220 (3): 760- 772. doi: 10.1111/nph.14565
	Escobar-Bravo R, Alba J M, Pons C, et al. A jasmonate-inducible defense trait transferred from wild into cultivated tomato establishes increased whitefly resistance and reduced viral disease incidence. Frontiers in Plant Science, 2016, 7, 1732.
	Fabisch T, Gershenzon J, Unsicker S B. Specificity of herbivore defense responses in a woody plant, black poplar (Populus nigra). Journal of Chemical Ecology, 2019, 45 (2): 162- 177. doi: 10.1007/s10886-019-01050-y
	Fan D, Ran L Y, Hu J, et al. miR319a/TCP module and DELLA protein regulate trichome initiation synergistically and improve insect defenses in Populus tomentosa. New Phytologist, 2020, 227 (3): 867- 883. doi: 10.1111/nph.16585
	Fellenberg C, Corea O, Yan L H, et al. Discovery of salicyl benzoate UDP-glycosyltransferase, a central enzyme in poplar salicinoid phenolic glycoside biosynthesis. Plant Journal, 2020, 102 (1): 99- 115. doi: 10.1111/tpj.14615
	Fladung M, Hoenicka H, Raj Ahuja M. Genomic stability and long-term transgene expression in poplar. Transgenic Research, 2013, 22 (6): 1167- 1178. doi: 10.1007/s11248-013-9719-2
	Gaur R K, de Abreu I N, Albrectsen B R. Compensatory phenolic induction dynamics in aspen after aphid infestation. Scientific Reports, 2022, 12 (1): 9582. doi: 10.1038/s41598-022-13225-x
	Gordon H, Fellenberg C, Lackus N D, et al. CRISPR/Cas9 disruption of UGT71L1 in poplar connects salicinoid and salicylic acid metabolism and alters growth and morphology. Plant Cell, 2022, 34 (8): 2925- 2947. doi: 10.1093/plcell/koac135
	Han G L, Li Y X, Yang Z R, et al. Molecular mechanisms of plant trichome development. Frontiers in Plant Science, 2022, 13, 910228. doi: 10.3389/fpls.2022.910228
	Hoffman N E. 2021. Revisions to USDA biotechnology regulations: the SECURE rule. Proceedings of the National Academy of Sciences of the United States of America, 118(22): e2004841118.
	Hu J J, Zhang J, Chen X L, et al. An empirical assessment of transgene flow from a Bt transgenic poplar plantation. PLoS ONE, 2017, 12 (1): e0170201. doi: 10.1371/journal.pone.0170201
	Huang Y L, Zhen Z X, Zhe C, et al. Growth and arthropod community characteristics of transgenic poplar 741 in an experimental forest. Industrial Crops and Products, 2021, 162 (4): 113284.
	Irmisch S, McCormick A C, Günther J, et al. Herbivore-induced poplar cytochrome P450 enzymes of the CYP71 family convert aldoximes to nitriles which repel a generalist caterpillar. Plant Journal, 2014, 80 (6): 1095- 1107. doi: 10.1111/tpj.12711
	Irmisch S, McCormick A C, Boeckler G A, et al. Two herbivore-induced cytochrome P450 enzymes CYP79D6 and CYP79D7 catalyze the formation of volatile aldoximes involved in poplar defense. Plant Cell, 2013a, 25 (11): 4737- 4754. doi: 10.1105/tpc.113.118265
	Irmisch S, Unsicker S B, Gershenzon J, et al. Identification and characterization of CYP79D6v4, a cytochrome P450 enzyme producing aldoximes in black poplar (Populus nigra). Plant Signaling & Behavior, 2013b, 8 (12): e27640. doi: 10.4161/psb.27640
	Kulasekaran S, Cerezo-Medina S, Harflett C, et al. A willow UDP-glycosyltransferase involved in salicinoid biosynthesis. Journal of Experimental Botany, 2021, 72 (5): 1634- 1648. doi: 10.1093/jxb/eraa562
	Lenz P R N, Nadeau S, Mottet M J, et al. Multi-trait genomic selection for weevil resistance, growth, and wood quality in Norway spruce. Evolutionary Applications, 2020, 13 (1): 76- 94. doi: 10.1111/eva.12823
	Li J X, Wang X X, Jiang R, et al. Phytohormone-based regulation of trichome development. Frontiers in Plant Science, 2021, 12, 734776. doi: 10.3389/fpls.2021.734776
	Lu L, Zhang Y, He Q Z H, et al. MTA, an RNA m⁶A methyltransferase, enhances drought tolerance by regulating the development of trichomes and roots in poplar. International Journal of Molecular Sciences, 2020, 21 (7): E2462. doi: 10.3390/ijms21072462
	Major I T, Constabel C P 2008. Functional analysis of the Kunitz trypsin inhibitor family in poplar reveals biochemical diversity and multiplicity in defense against herbivores. Plant Physiology, 146(3): 888–903.
	McCormick A C, Boeckler G A, Köllner T G, et al. The timing of herbivore-induced volatile emission in black poplar (Populus nigra) and the influence of herbivore age and identity affect the value of individual volatiles as cues for herbivore enemies. BMC Plant Biology, 2014, 14, 304. doi: 10.1186/s12870-014-0304-5
	McCormick A C, Unsicker S B, Gershenzon J. The specificity of herbivore-induced plant volatiles in attracting herbivore enemies. Trends in Plant Science, 2012, 17 (5): 303- 310. doi: 10.1016/j.tplants.2012.03.012
	McKenna D D, Scully E D, Pauchet Y, et al. Genome of the Asian longhorned beetle (Anoplophora glabripennis), a globally significant invasive species, reveals key functional and evolutionary innovations at the beetle–plant interface. Genome Biology, 2016, 17 (1): 227. doi: 10.1186/s13059-016-1088-8
	Meshkova V, Zhupinska K, Borysenko O, et al. Possible factors of poplar susceptibility to large poplar borer infestation. Forests, 2024, 15 (5): 882. doi: 10.3390/f15050882
	Mhoswa L, O’Neill M M, Mphahlele M M, et al. A genome-wide association study for resistance to the insect pest Leptocybe invasa in Eucalyptus grandis reveals genomic regions and positional candidate defense genes. Plant and Cell Physiology, 2020, 61 (7): 1285- 1296. doi: 10.1093/pcp/pcaa057
	Mithöfer A, Boland W. Plant defense against herbivores: chemical aspects. Annual Review of Plant Biology, 2012, 63, 431- 450. doi: 10.1146/annurev-arplant-042110-103854
	Müller N A, Kersten B, Fladung M, et al. RNA-seq of eight different poplar clones reveals conserved up-regulation of gene expression in response to insect herbivory. BMC Genomics, 2019, 20 (1): 673. doi: 10.1186/s12864-019-6048-8
	Nvsvrot T, Xia W X, Xiao Z A, et al. Combining QTL mapping with genome resequencing identifies an indel in an R gene that is associated with variation in leaf rust disease resistance in poplar. Phytopathology, 2020, 110 (4): 900- 906. doi: 10.1094/PHYTO-10-19-0402-R
	Okumura S, Sawada M, Park Y W, et al. Transformation of poplar (Populus alba) plastids and expression of foreign proteins in tree chloroplasts. Transgenic Research, 2006, 15 (5): 637- 646. doi: 10.1007/s11248-006-9009-3
	Philippe R N, Ralph S G, Külheim C, et al. Poplar defense against insects: genome analysis, full-length cDNA cloning, and transcriptome and protein analysis of the poplar Kunitz-type protease inhibitor family. New Phytologist, 2009, 184 (4): 865- 884. doi: 10.1111/j.1469-8137.2009.03028.x
	Plett J M, Wilkins O, Campbell M M, et al. Endogenous overexpression of Populus MYB186 increases trichome density, improves insect pest resistance, and impacts plant growth. Plant Journal, 2010, 64 (3): 419- 432. doi: 10.1111/j.1365-313X.2010.04343.x
	Qi T C, Song S S, Ren Q C, et al. The Jasmonate-ZIM-domain proteins interact with the WD-Repeat/bHLH/MYB complexes to regulate Jasmonate-mediated anthocyanin accumulation and trichome initiation in Arabidopsis thaliana. Plant Cell, 2011, 23 (5): 1795- 1814. doi: 10.1105/tpc.111.083261
	Ren Y C, Huang Y L, Zhang J, et al. Application of polygene polymerization for insect-resistant poplar breeding. Forestry Research, 2022, 2, 3.
	Ren Y C, Zhou X L, Dong Y, et al. Exogenous gene expression and insect resistance in dual Bt toxin Populus × euramericana 'Neva' transgenic plants. Frontiers in Plant Science, 2021, 12, 660226. doi: 10.3389/fpls.2021.660226
	Rieger M A, Lamond M, Preston C, et al. Pollen-mediated movement of herbicide resistance between commercial canola fields. Science, 2002, 296 (5577): 2386- 2388. doi: 10.1126/science.1071682
	Sepúlveda S L, Neale D B, Holliday J A, et al. GWAS on the attack by aspen borer Saperda calcarata on black cottonwood trees reveals a response mechanism involving secondary metabolism and independence of tree architecture. Forests, 2023, 14 (6): 1129. doi: 10.3390/f14061129
	Simon S J, Tschaplinski T J, LeBoldus J M, et al. Host plant genetic control of associated fungal and insect species in a Populus hybrid cross. Ecology and Evolution, 2020, 10 (11): 5119- 5134. doi: 10.1002/ece3.6266
	Tan W R, Han Q, Li Y, et al. A HAT1-DELLA signaling module regulates trichome initiation and leaf growth by achieving gibberellin homeostasis. New Phytologist, 2021, 231 (3): 1220- 1235. doi: 10.1111/nph.17422
	Unsicker S B, Gershenzon J, Köllner T G. Beetle feeding induces a different volatile emission pattern from black poplar foliage than caterpillar herbivory. Plant Signaling & Behavior, 2015, 10 (3): e987522. doi: 10.4161/15592324.2014.987522
	Wang G Y, Dong Y, Liu X J, et al. The current status and development of insect-resistant genetically engineered poplar in China. Frontiers in Plant Science, 2018, 9, 1408. doi: 10.3389/fpls.2018.01408
	Wang L J, Qu L J, Hu J J, et al. Metabolomics reveals constitutive metabolites that contribute resistance to fall webworm (Hyphantria cunea) in Populus deltoides. Environmental and Experimental Botany, 2017, 136 (4): 31- 40.
	Wang S J, Liu J X, Dong Y, et al. Dynamic monitoring of the impact of insect-resistant transgenic poplar field stands on arthropod communities. Forest Ecology and Management, 2022, 505 (2): 11992.
	Wang Y, Yang Y, Wang F, et al. Growth adaptability and foreign gene stability of TaLEA transgenic Populus simonii × nigra. Annals of Forest Science, 2021, 78, 42. doi: 10.1007/s13595-021-01038-3
	Wang Y B, Zhang W X, Ding C J, et al. Endophytic communities of transgenic poplar were determined by the environment and niche rather than by transgenic events. Frontiers in Microbiology, 2019, 10, 588. doi: 10.3389/fmicb.2019.00588
	Wei H, Xu C, Movahedi A, et al. Characterization and function of 3-hydroxy-3-methylglutaryl-CoA reductase in Populus trichocarpa: overexpression of PtHMGR enhances terpenoids in transgenic poplar. Frontiers in Plant Science, 2019, 10, 1476. doi: 10.3389/fpls.2019.01476
	Wu N N, Zhang S F, Li X W, et al. Fall webworm genomes yield insights into rapid adaptation of invasive species. Nature Ecology & Evolution, 2019a, 3 (1): 105- 115.
	Wu Y Y, Xu L T, Chang L, et al. Bacillus thuringiensis cry1C expression from the plastid genome of poplar leads to high mortality of leaf-eating caterpillars. Tree Physiology, 2019b, 39 (9): 1525- 1532. doi: 10.1093/treephys/tpz073
	Xu C, Wei H, Wang L K, et al. Optimization of the cry1Ah1 sequence enhances the hyper-resistance of transgenic poplars to Hyphantria cunea. Frontiers in Plant Science, 2019, 10, 335. doi: 10.3389/fpls.2019.00335
	Xu S J, Zhang Y Q, Li S C, et al. Plastid-expressed Bacillus thuringiensis (Bt) cry3Bb confers high mortality to a leaf eating beetle in poplar. Plant Cell Reports, 2020, 39 (3): 317- 323. doi: 10.1007/s00299-019-02492-0
	Yang S D, Wang G, Niu M H, et al. Impacts of AlaAT3 transgenic poplar on rhizosphere soil chemical properties, enzyme activity, bacterial community, and metabolites under two nitrogen conditions. GM Crops & Food, 2024, 15 (1): 1- 15.
	Yoshida Y, Sano R, Wada T, et al. Jasmonic acid control of GLABRA3 links inducible defense and trichome patterning in Arabidopsis. Development, 2009, 136 (6): 1039- 1048. doi: 10.1242/dev.030585
	Yu X Y, Lu B, Dong Y, et al. Cloning and functional identification of PeWRKY41 from Populus × euramericana. Industrial Crops and Products, 2022, 175 (1): 114279.
	Yu X T, Pan Y, Dong Y, et al. Cloning and overexpression of PeWRKY31 from Populus × euramericana enhances salt and biological tolerance in transgenic Nicotiana. BMC Plant Biology, 2021, 21 (1): 80. doi: 10.1186/s12870-021-02856-3
	Zhang B Y, Chen M, Zhang X F, et al. Expression of Bt-Cry3A in transgenic Populus alba × P. glandulosa and its effects on target and non-target pests and the arthropod community. Transgenic Research, 2011, 20 (3): 523- 532. doi: 10.1007/s11248-010-9434-1
	Zhang Z J, Huang Y L, Dong Y, et al. Effect of T-DNA integration on growth of transgenic Populus × euramericana cv. Neva underlying field stands. International Journal of Molecular Sciences, 2023, 24 (16): 12952. doi: 10.3390/ijms241612952
	Zhou C, Zhang Q, Chen Y, et al. Balancing selection and wild gene pool contribute to resistance in global rice germplasm against planthopper. Journal of Integrative Plant Biology, 2021, 63 (10): 1695- 1711. doi: 10.1111/jipb.13157
	Zhou F W, Wu H T, Chen Y N, et al. 2023. Function and molecular mechanism of a poplar placenta limited MIXTA gene in regulating differentiation of plant epidermal cells. International Journal of Biological Macromolecules, 242(Pt 2): 124743.
	Zhou H L, Wang X Y, Mo Y, et al. Genetic analysis and fine mapping of the gall midge resistance gene Gm5 in rice (Oryza sativa L.). Theoretical and Applied Genetics, 2020a, 133 (6): 2021- 2033. doi: 10.1007/s00122-020-03575-3
	Zhou X L, Dong Y, Zhang Q, et al. Expression of multiple exogenous insect resistance and salt tolerance genes in Populus nigra L. Frontiers in Plant Science, 2020b, 11, 1123. doi: 10.3389/fpls.2020.01123
	Zhou X L, Ren Y C, Wang S J, et al. T-DNA integration and its effect on gene expression in dual Bt gene transgenic Populus x euramericana cv. Neva. Industrial Crops and Products, 2022, 178, 114636. doi: 10.1016/j.indcrop.2022.114636
	Zuo L H, Yang R L, Zhen Z X, et al. A 5-year field study showed no apparent effect of the Bt transgenic 741 poplar on the arthropod community and soil bacterial diversity. Scientific Reports, 2018, 8 (1): 1956. doi: 10.1038/s41598-018-20322-3

[1]	Lingyu Yang,Wenguang Shi,Zhibin Luo. Characteristics of Ectomycorrhizal Fungi Paxillus involutus Promoting Nitrogen Uptake and Utilization of Its Host Populus tremula × Populus alba [J]. Scientia Silvae Sinicae, 2024, 60(9): 69-79.
[2]	Xiaolin Qiu,Shumin Wang,Lu Yu,Yuchen Yang,Dianguang Xiong,Chengming Tian. Functional of SNARE Protein CcNyv1 in Cytospora chrysosperma [J]. Scientia Silvae Sinicae, 2024, 60(9): 90-98.
[3]	Yingqi He,Lufei Wang,Yamei Zhang,Yanglun Yu,Wenji Yu. Effect of Compression Ratios on the Surface Hardness of Poplar Wood Scrimber [J]. Scientia Silvae Sinicae, 2024, 60(9): 141-149.
[4]	Aoyu Wang,Youzheng Guo,Tan Deng,Yang Liu,Nan Di,Jie Duan,Ximeng Li,Benye Xi. Comparison of Several Methods for Evaluating Plant Water Regulation Strategies [J]. Scientia Silvae Sinicae, 2024, 60(8): 109-119.
[5]	Kong Yue,Xiang Li,Xinlei Shi,Xuekai Jiao,Peng Wu,Zhongfeng Zhang,Guoliang Dong,Yuanjin Fang. Effects of Thermal Pretreatment on Lateral Performance of Poplar Cross-Laminated Timber Shear Walls [J]. Scientia Silvae Sinicae, 2024, 60(7): 117-128.
[6]	Lei Xu,Xiaoyun Wu,Jiang Lü,Yun Shi,Mengxun Zhu,Hang Xu,Zhiqiang Zhang. Impacts of Diffuse Radiation Fraction on Energy Partitioning in a Poplar Plantation in the North China Plain [J]. Scientia Silvae Sinicae, 2024, 60(3): 100-110.
[7]	Meihong Liu,Qiming Yan,Longbo Zi,Yafang Lei,Li Yan. Physical and Mechanical Properties of Furfuryl Alcohol-Epoxidized Vegetable Oils Composite Modified Poplar Wood [J]. Scientia Silvae Sinicae, 2024, 60(11): 149-159.
[8]	Jiaming Wan,Jiang Lü,Yun Shi,Hang Xu,Zhiqiang Zhang. Effects of Diffuse Radiation on the Gross Primary Productivity of a Poplar Plantation [J]. Scientia Silvae Sinicae, 2023, 59(5): 1-10.
[9]	Lu Han,Han Zhao,Wei Wang,Wenhui Liu,Zaimin Jiang,Jing Cai. Hydraulic Vulnerability Segmentation and Its Correlation with Growth in Hybrid Poplar [J]. Scientia Silvae Sinicae, 2023, 59(3): 94-103.
[10]	Weifeng Wang,Yuqi Zhao,Miaoqin Gao,Yuzheng Zong,Xingyu Hao. Leaf Photosynthesis and Carbon and Nitrogen Distribution of Populus×popularis‘35-44’ Young Cuttings in Response to Elevated CO₂ Concentration and Temperature [J]. Scientia Silvae Sinicae, 2023, 59(2): 40-47.
[11]	Ruirui Zhao,Yong Liu,Kai Wang. Effects of Biochar and Manure on Wood Decomposition and Soil Enzyme Activities Related Soil Nutrient Cycling in a triploid Populus tomentosa Plantation [J]. Scientia Silvae Sinicae, 2023, 59(11): 1-11.
[12]	Wei Wang,Han Zhao,Xin Huang,Zhuoliang Hou,Zaimin Jiang,Jing Cai. Relationship Between Leaf Hydraulic and Economic Traits and Biomass of Poplar Clones [J]. Scientia Silvae Sinicae, 2023, 59(10): 89-98.
[13]	Minxia Ren,Tan Li,Ziheng Zhang,Yuexia Zeng,Lifeng Wang,Minsheng Yang,Junxia Liu. Effects of Transgenic BtCry1Ac and API gene in Poplar 107 on Diversity and Stability of Arthropod Community [J]. Scientia Silvae Sinicae, 2022, 58(4): 110-118.
[14]	Youjing Zhang,Yueyang Li,Han Zhao,Yuwan Cheng,Wei Wang,Zaimin Jiang,Jing Cai. Relationship between Hydraulic Efficiency and Gas Exchange and Growth of Six Poplar Clones [J]. Scientia Silvae Sinicae, 2022, 58(11): 118-126.
[15]	Weixi Zhang,Yanbo Wang,Changjun Ding,Wenxu Zhu,Xiaohua Su. Detection of Horizontal Transfer of the Exogenous Gene in Adult Trees of Transgenic Populus alba × P. berolinensis in a Field Trial and Successive Years of Monitoring of Soil Microorganism [J]. Scientia Silvae Sinicae, 2022, 58(1): 52-61.

Advance of Poplar Molecular Breeding with Insect Resistance and Transgenic Biosafety Assessment Research

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 3

References 0

Related Articles 15

Recommended Articles

Metrics

Comments